We narrowed to 18,395 results for: Tec

1. pJT331 Lenti pEF-dCas9-3XFLAG-Q+N+Z+F-T2A-tagBFP-P2A-Blast

   Plasmid

   #195538

   Purpose

   dCas9 effector fusion for manipulating transcription

   Depositor

   Lacra Bintu, Michael Bassik

   Article

   Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

   Insert

   Lenti pEF-dCas9-3XFLAG-Q+N+Z+F-T2A-tagBFP-P2A-Blast
   

   Use

   CRISPR and Lentiviral

   Tags

   3XFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   pEF1a

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pJT360 AAV-Rapamycin Inducible-noActivator Domain -Citrine

   Plasmid

   #202058

   Purpose

   Inducible AAV with reporter gene and cloning site to add activator domains

   Depositor

   Lacra Bintu, Michael Bassik

   Article

   Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

   Insert

   AAV-Rapamycin Inducible-noActivator Domain -Citrine
   

   Use

   AAV; Aav with reporter

   Tags

   Expression

   Mammalian

   Mutation

   ITRs intact

   Promoter

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pmCherry2-GNB1-T2A-mCherry2-GNG2-IRES-GNAI1-mEYFP(Q69K)

   Plasmid

   #190755

   Purpose

   Expression of trimeric G protein with mCherry2 (GNB1 and GNG2) or mEYFP(Q69K) (GNAI1) tags.

   Depositor

   Dave Piston

   Article

   Foust et al bioRxiv 2021.11.03.467169

   Inserts

   GNB1 (GNB1 Human)
   GNG2 (GNG2 Human)
   GNAI1 (GNAI1 Human)
   

   Use

   Tags

   mCherry2 and mEYFP(Q69K)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLV-EF1A-WWE-HA-nLuc-FLAG-T2A-WWE-myc-cLuc-FLAG-IRES-Puro

   Plasmid

   #187611

   Purpose

   Split luciferase construct with nLuc fused to C-terminus of WWE domain, linked by T2A to cLuc fused to C-terminus of WWE domain, linked by IRES to puromycin resistance cassette

   Depositor

   Robert Sobol

   Article

   Koczor et al Cancers (Basel). 2022 Jul 28;14(15). pii

   Inserts

   WWE domain with C-terminal nLuc
   WWE domain with C-terminal cLuc
   

   Use

   Lentiviral

   Tags

   FLAG, HA, Split luciferase (cLuc), Split lucifera…

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available since

   Sept. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLV-EF1A-WWE(R163A)-HA-nLuc-FLAG-T2A-WWE-myc-cLuc-FLAG-IRES-Puro

   Plasmid

   #187612

   Purpose

   Split luciferase construct with nLuc fused to C-terminus of WWE domain with Arg163Ala mutation, linked by T2A to cLuc fused to C-terminus of WWE domain, linked by IRES to puromycin resistance cassette

   Depositor

   Robert Sobol

   Article

   Koczor et al Cancers (Basel). 2022 Jul 28;14(15). pii

   Inserts

   WWE domain (R163A) with C-terminal nLuc
   WWE domain with C-terminal cLuc
   

   Use

   Lentiviral

   Tags

   FLAG, HA, Split luciferase (cLuc), Split lucifera…

   Expression

   Mammalian

   Mutation

   Arg163Ala

   Promoter

   EF1A

   Available since

   Sept. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pv6_MECP2_MBD-R133C

   Plasmid

   #179409

   Purpose

   Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of MECP2_MBD(R133C)

   Depositor

   Tuncay Baubec

   Article

   Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

   Insert

   MECP2_MBD(R133C) (Mecp2 Mouse)
   

   Use

   Tags

   AviTag and EGFP

   Expression

   Mammalian

   Mutation

   MBD R133C

   Promoter

   CAGGS

   Available since

   Sept. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pv6_MECP2_MBD-T158M

   Plasmid

   #179410

   Purpose

   Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of MECP2_MBD(T158M)

   Depositor

   Tuncay Baubec

   Article

   Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

   Insert

   MECP2_MBD(T158M) (Mecp2 Mouse)
   

   Use

   Tags

   AviTag and EGFP

   Expression

   Mammalian

   Mutation

   MBD T158M

   Promoter

   CAGGS

   Available since

   Aug. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pIG-717pUC19-tNGFR-P2A-CD3E

   Plasmid

   #186111

   Purpose

   Knockin of truncated NGFR to target gene

   Depositor

   Alexander Marson

   Article

   Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

   Insert

   CD3E (CD3E Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   WT with tNGFR sequence

   Promoter

   Available since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pIG-745pUC19-tNGFR-P2A-CD4

   Plasmid

   #186115

   Purpose

   Knockin of truncated NGFR to target gene

   Depositor

   Alexander Marson

   Article

   Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

   Insert

   CD4 (CD4 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   WT with tNGFR sequence

   Promoter

   Available since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pIG-744pUC19-tNGFR-P2A-CD2

    Plasmid

    #186118

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    CD2 (CD2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    WT with tNGFR sequence

    Promoter

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pIG-716pUC19-tNGFR-P2A-CD3G

    Plasmid

    #186090

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    CD3G (CD3G Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    WT with tNGFR sequence

    Promoter

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pIG-718pUC19-tNGFR-P2A-CD3D

    Plasmid

    #186100

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    CD3D (CD3D Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    WT with tNGFR sequence

    Promoter

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pTR-348 pUC19-tNGFR-P2A-ICOS N

    Plasmid

    #186066

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    ICOS (ICOS Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    WT with tNGFR sequence

    Promoter

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTR-375 tNGFR-P2A-ITGB7

    Plasmid

    #186069

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    ITGB7 (ITGB7 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    WT with tNGFR sequence

    Promoter

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTR-377 tNGFR-P2A-CD5

    Plasmid

    #186071

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    CD5 (CD5 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    WT with tNGFR sequence

    Promoter

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTR-379 tNGFR-P2A-CD48

    Plasmid

    #186073

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    CD48 (CD48 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    WT with tNGFR sequence

    Promoter

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTR-452 tNGFR-P2A-Lck

    Plasmid

    #186080

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    LCK (LCK Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    WT with tNGFR sequence

    Promoter

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTR-342 pUC19-tNGFR-P2A-CD28 N

    Plasmid

    #186056

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    CD28 (CD28 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    WT with tNGFR sequence

    Promoter

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTR-449 tNGFR-P2A-CCR2

    Plasmid

    #186057

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    CCR2 (CCR2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    WT with tNGFR sequence

    Promoter

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. I282L

    Plasmid

    #184308

    Purpose

    To study dynamics of G-actin using NMR spectroscopy.

    Depositor

    Michael Rosen

    Article

    Ali et al Nat Struct Mol Biol. 2022 Apr;29(4):320-

    Insert

    Act5C (Act5C Fly)
    

    Use

    Tags

    B-Thymosin followedby hexahistidine tag

    Expression

    Yeast

    Mutation

    I282L

    Promoter

    AOX1

    Available since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. I175L

    Plasmid

    #184322

    Purpose

    To study dynamics of G-actin using NMR spectroscopy.

    Depositor

    Michael Rosen

    Article

    Ali et al Nat Struct Mol Biol. 2022 Apr;29(4):320-

    Insert

    Act5C (Act5C Fly)
    

    Use

    Tags

    Hexahistidine followed by B-thymosin

    Expression

    Yeast

    Mutation

    I175L

    Promoter

    AOX1

    Available since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. K118N

    Plasmid

    #184324

    Purpose

    To study dynamics of G-actin using NMR spectroscopy.

    Depositor

    Michael Rosen

    Article

    Ali et al Nat Struct Mol Biol. 2022 Apr;29(4):320-

    Insert

    Act5C (Act5C Fly)
    

    Use

    Tags

    Hexahistidine followed by B-thymosin

    Expression

    Yeast

    Mutation

    K118N

    Promoter

    AOX1

    Available since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. I212L

    Plasmid

    #184304

    Purpose

    To study dynamics of G-actin using NMR spectroscopy.

    Depositor

    Michael Rosen

    Article

    Ali et al Nat Struct Mol Biol. 2022 Apr;29(4):320-

    Insert

    Act5C (Act5C Fly)
    

    Use

    Tags

    B-Thymosin followedby hexahistidine tag

    Expression

    Yeast

    Mutation

    I212L

    Promoter

    AOX1

    Available since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. I327L

    Plasmid

    #184311

    Purpose

    To study dynamics of G-actin using NMR spectroscopy.

    Depositor

    Michael Rosen

    Article

    Ali et al Nat Struct Mol Biol. 2022 Apr;29(4):320-

    Insert

    Act5C (Act5C Fly)
    

    Use

    Tags

    B-Thymosin followedby hexahistidine tag

    Expression

    Yeast

    Mutation

    I327L

    Promoter

    AOX1

    Available since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. I309L

    Plasmid

    #184317

    Purpose

    To study dynamics of G-actin using NMR spectroscopy.

    Depositor

    Michael Rosen

    Article

    Ali et al Nat Struct Mol Biol. 2022 Apr;29(4):320-

    Insert

    Act5C (Act5C Fly)
    

    Use

    Tags

    Hexahistidine followed by B-thymosin

    Expression

    Yeast

    Mutation

    I309L

    Promoter

    AOX1

    Available since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. I330L

    Plasmid

    #184320

    Purpose

    To study dynamics of G-actin using NMR spectroscopy.

    Depositor

    Michael Rosen

    Article

    Ali et al Nat Struct Mol Biol. 2022 Apr;29(4):320-

    Insert

    Act5C (Act5C Fly)
    

    Use

    Tags

    Hexahistidine followed by B-thymosin

    Expression

    Yeast

    Mutation

    I330L

    Promoter

    AOX1

    Available since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. I151L

    Plasmid

    #184300

    Purpose

    To study dynamics of G-actin using NMR spectroscopy.

    Depositor

    Michael Rosen

    Article

    Ali et al Nat Struct Mol Biol. 2022 Apr;29(4):320-

    Insert

    Act5C (Act5C Fly)
    

    Use

    Tags

    B-Thymosin followedby hexahistidine tag

    Expression

    Yeast

    Mutation

    I151L

    Promoter

    AOX1

    Available since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. I369L

    Plasmid

    #184318

    Purpose

    To study dynamics of G-actin using NMR spectroscopy.

    Depositor

    Michael Rosen

    Article

    Ali et al Nat Struct Mol Biol. 2022 Apr;29(4):320-

    Insert

    Act5C (Act5C Fly)
    

    Use

    Tags

    Hexahistidine followed by B-thymosin

    Expression

    Yeast

    Mutation

    I369L

    Promoter

    AOX1

    Available since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. I274L

    Plasmid

    #184307

    Purpose

    To study dynamics of G-actin using NMR spectroscopy.

    Depositor

    Michael Rosen

    Article

    Ali et al Nat Struct Mol Biol. 2022 Apr;29(4):320-

    Insert

    Act5C (Act5C Fly)
    

    Use

    Tags

    B-Thymosin followedby hexahistidine tag

    Expression

    Yeast

    Mutation

    I274L

    Promoter

    AOX1

    Available since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. I122L

    Plasmid

    #184299

    Purpose

    To study dynamics of G-actin using NMR spectroscopy.

    Depositor

    Michael Rosen

    Article

    Ali et al Nat Struct Mol Biol. 2022 Apr;29(4):320-

    Insert

    Act5C (Act5C Fly)
    

    Use

    Tags

    B-Thymosin followedby hexahistidine tag

    Expression

    Yeast

    Mutation

    I122L

    Promoter

    AOX1

    Available since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. I85L

    Plasmid

    #184298

    Purpose

    To study dynamics of G-actin using NMR spectroscopy.

    Depositor

    Michael Rosen

    Article

    Ali et al Nat Struct Mol Biol. 2022 Apr;29(4):320-

    Insert

    Act5C (Act5C Fly)
    

    Use

    Tags

    B-Thymosin followedby hexahistidine tag

    Expression

    Yeast

    Mutation

    I85L

    Promoter

    AOX1

    Available since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. I75V

    Plasmid

    #184315

    Purpose

    To study dynamics of G-actin using NMR spectroscopy.

    Depositor

    Michael Rosen

    Article

    Ali et al Nat Struct Mol Biol. 2022 Apr;29(4):320-

    Insert

    Act5C (Act5C Fly)
    

    Use

    Tags

    B-Thymosin followedby hexahistidine tag

    Expression

    Yeast

    Mutation

    I75V

    Promoter

    AOX1

    Available since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. I345L

    Plasmid

    #184323

    Purpose

    To study dynamics of G-actin using NMR spectroscopy.

    Depositor

    Michael Rosen

    Article

    Ali et al Nat Struct Mol Biol. 2022 Apr;29(4):320-

    Insert

    Act5C (Act5C Fly)
    

    Use

    Tags

    Hexahistidine followed by B-thymosin

    Expression

    Yeast

    Mutation

    I345L

    Promoter

    AOX1

    Available since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. I165L

    Plasmid

    #184301

    Purpose

    To study dynamics of G-actin using NMR spectroscopy.

    Depositor

    Michael Rosen

    Article

    Ali et al Nat Struct Mol Biol. 2022 Apr;29(4):320-

    Insert

    Act5C (Act5C Fly)
    

    Use

    Tags

    B-Thymosin followedby hexahistidine tag

    Expression

    Yeast

    Mutation

    I165L

    Promoter

    AOX1

    Available since

    May 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. I329L

    Plasmid

    #184312

    Purpose

    To study dynamics of G-actin using NMR spectroscopy.

    Depositor

    Michael Rosen

    Article

    Ali et al Nat Struct Mol Biol. 2022 Apr;29(4):320-

    Insert

    Act5C (Act5C Fly)
    

    Use

    Tags

    B-Thymosin followedby hexahistidine tag

    Expression

    Yeast

    Mutation

    I329L

    Promoter

    AOX1

    Available since

    May 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. I341L

    Plasmid

    #184313

    Purpose

    To study dynamics of G-actin using NMR spectroscopy.

    Depositor

    Michael Rosen

    Article

    Ali et al Nat Struct Mol Biol. 2022 Apr;29(4):320-

    Insert

    Act5C (Act5C Fly)
    

    Use

    Tags

    B-Thymosin followedby hexahistidine tag

    Expression

    Yeast

    Mutation

    I341L

    Promoter

    AOX1

    Available since

    May 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. I34L

    Plasmid

    #184296

    Purpose

    To study dynamics of G-actin using NMR spectroscopy.

    Depositor

    Michael Rosen

    Article

    Ali et al Nat Struct Mol Biol. 2022 Apr;29(4):320-

    Insert

    Act5C (Act5C Fly)
    

    Use

    Tags

    B-Thymosin followedby hexahistidine tag

    Expression

    Yeast

    Mutation

    I34L

    Promoter

    AOX1

    Available since

    May 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. I208L

    Plasmid

    #184303

    Purpose

    To study dynamics of G-actin using NMR spectroscopy.

    Depositor

    Michael Rosen

    Article

    Ali et al Nat Struct Mol Biol. 2022 Apr;29(4):320-

    Insert

    Act5C (Act5C Fly)
    

    Use

    Tags

    B-Thymosin followedby hexahistidine tag

    Expression

    Yeast

    Mutation

    I208L

    Promoter

    AOX1

    Available since

    May 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. I250L

    Plasmid

    #184306

    Purpose

    To study dynamics of G-actin using NMR spectroscopy.

    Depositor

    Michael Rosen

    Article

    Ali et al Nat Struct Mol Biol. 2022 Apr;29(4):320-

    Insert

    Act5C (Act5C Fly)
    

    Use

    Tags

    B-Thymosin followedby hexahistidine tag

    Expression

    Yeast

    Mutation

    I250L

    Promoter

    AOX1

    Available since

    May 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. I248L

    Plasmid

    #184305

    Purpose

    To study dynamics of G-actin using NMR spectroscopy.

    Depositor

    Michael Rosen

    Article

    Ali et al Nat Struct Mol Biol. 2022 Apr;29(4):320-

    Insert

    Act5C (Act5C Fly)
    

    Use

    Tags

    B-Thymosin followedby hexahistidine tag

    Expression

    Yeast

    Mutation

    I248L

    Promoter

    AOX1

    Available since

    May 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. I64L

    Plasmid

    #184321

    Purpose

    To study dynamics of G-actin using NMR spectroscopy.

    Depositor

    Michael Rosen

    Article

    Ali et al Nat Struct Mol Biol. 2022 Apr;29(4):320-

    Insert

    Act5C (Act5C Fly)
    

    Use

    Tags

    Hexahistidine followed by B-thymosin

    Expression

    Yeast

    Mutation

    I64L

    Promoter

    AOX1

    Available since

    May 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. I192L

    Plasmid

    #184302

    Purpose

    To study dynamics of G-actin using NMR spectroscopy.

    Depositor

    Michael Rosen

    Article

    Ali et al Nat Struct Mol Biol. 2022 Apr;29(4):320-

    Insert

    Act5C (Act5C Fly)
    

    Use

    Tags

    B-Thymosin followedby hexahistidine tag

    Expression

    Yeast

    Mutation

    I192L

    Promoter

    AOX1

    Available since

    May 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. I71L

    Plasmid

    #184297

    Purpose

    To study dynamics of G-actin using NMR spectroscopy.

    Depositor

    Michael Rosen

    Article

    Ali et al Nat Struct Mol Biol. 2022 Apr;29(4):320-

    Insert

    Act5C (Act5C Fly)
    

    Use

    Tags

    B-Thymosin followedby hexahistidine tag

    Expression

    Yeast

    Mutation

    I71L

    Promoter

    AOX1

    Available since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pDONR221-ZNF385A

    Plasmid

    #101615

    Purpose

    Donor Vector containing ZNF385A transcription factor, part of the Human TFome Collection

    Depositor

    George Church

    Article

    Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

    Insert

    ZNF385A (ZNF385A Human)
    

    Use

    Gateway shuttling vector

    Tags

    Expression

    Mutation

    Last nucleotide of stop codon removed to allow fo…

    Promoter

    Available since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pDONR221-FOXD4L3

    Plasmid

    #101491

    Purpose

    Donor Vector containing FOXD4L3 transcription factor, part of the Human TFome Collection

    Depositor

    George Church

    Article

    Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

    Insert

    FOXD4L3 (FOXD4L3 Human)
    

    Use

    Gateway shuttling vector

    Tags

    Expression

    Mutation

    Last nucleotide of stop codon removed to allow fo…

    Promoter

    Available since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. SITS-MmCav1-APEX2

    Plasmid

    #170526

    Purpose

    Vector expression of MmCav1-APEX2 in Leishmania cell free system. Parton lab clone IYW

    Depositor

    Rob Parton

    Article

    Rae et al Elife. 2021 Apr 27;10. pii: 64630. doi:

    Insert

    Caveolin1 (Cav1 Mouse)
    

    Use

    Leishmania cell free expression clone

    Tags

    APEX2 and Lifeact

    Expression

    Mutation

    Promoter

    Available since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pDONR221-ZNF197

    Plasmid

    #101547

    Purpose

    Donor Vector containing ZNF197 transcription factor, part of the Human TFome Collection

    Depositor

    George Church

    Article

    Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

    Insert

    ZNF197 (ZNF197 Human)
    

    Use

    Gateway shuttling vector

    Tags

    Expression

    Mutation

    Last nucleotide of stop codon removed to allow fo…

    Promoter

    Available since

    June 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pDONR223-ZNF302

    Plasmid

    #88279

    Purpose

    Donor Vector containing ZNF302 transcription factor, part of the Human TFome Collection

    Depositor

    Marc Vidal

    Article

    Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

    Insert

    ZNF302 (ZNF302 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Last nucleotide of stop codon removed to allow fo…

    Promoter

    Available since

    June 3, 2021

    Availability

    Academic Institutions and Nonprofits only

49. pDONR223-ZNF322

    Plasmid

    #88327

    Purpose

    Donor Vector containing ZNF322 transcription factor, part of the Human TFome Collection

    Depositor

    Marc Vidal

    Article

    Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

    Insert

    ZNF322 (ZNF322A Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Last nucleotide of stop codon removed to allow fo…

    Promoter

    Available since

    June 3, 2021

    Availability

    Academic Institutions and Nonprofits only

50. DHC1-mIAA7 Neo

    Plasmid

    #140543

    Purpose

    DHC1 tagging with mIAA7

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    DHC1 (DYNC1H1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge