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pCMV5 Flag-Smad1 (4SP/AP)

Plasmid

#14043

Depositor

Joan Massague

Publication

Kretzschmar et al Genes Dev. 1997 Apr 15. 11(8):984-95.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Smad1 (4SP/AP) (SMAD1 Human)

Tags

Flag

Expression

Mammalian

Mutation

Four Serine to Alanine mutations in the linker re…

Available since

May 10, 2007

Availability

Academic Institutions and Nonprofits only
pCMV human IRS-1 CAAX

Plasmid

#11238

Depositor

Ronald Kahn

Publication

Kriauciunas et al Mol Cell Biol. 2000 Sep . 20(18):6849-59

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

IRS-1 (IRS1 Human)

Tags

CAAX

Expression

Mammalian

Mutation

C-terminal prenylation motif makes IRS-1 localize…

Available since

May 25, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
SIVl

Plasmid

#81090

Purpose

Single Integration Vector LEU - Cloning vector for single integration into the LEU2 locus of S.cerevisiae containing two MCS.

Depositor

Serge Pelet

Publication

Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

Type

Empty backbone

Use

Cre/Lox

Expression

Yeast

Available since

Nov. 16, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMS050

Plasmid

#91826

Purpose

Codon-optimized mScarlet-I donor for the SapTrap cloning system

Depositor

Bob Goldstein

Publication

Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

Insert

mScarlet-I-C1

Use

CRISPR

Expression

Worm

Available since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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GFPZFN2-B2H

Plasmid

#26792

Depositor

Linzhao Cheng

Publication

Zou et al Cell Stem Cell. 2009 Jul 2. 5(1):97-110.

Insert

GFP-ZFN2

Expression

Mammalian

Available since

Nov. 29, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pEN_hUmiRc2

Plasmid

#25747

Purpose

Entry vector for cloning miR30-based shRNA driven by human U6 promoter.

Depositor

Iain Fraser

Publication

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Type

Empty backbone

Use

Gateway Cloning

Available since

July 13, 2010

Availability

Academic Institutions and Nonprofits only

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GST-TAZ

Plasmid

#19024

Depositor

Michael Yaffe

Publication

Park et al J Biol Chem. 2004 Apr 23. 279(17):17384-

Insert

TAZ (Wwtr1 Mouse)

Tags

GST

Expression

Bacterial

Available since

Aug. 25, 2008

Availability

Academic Institutions and Nonprofits only
pCI-neo-RL-ZEB1 200bmutx5

Plasmid

#35537

Depositor

Greg Goodall

Publication

Gregory et al Nat Cell Biol. 2008 May;10(5):593-601. E

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

3'UTR of ZEB1 (ZEB1 Human, Budding Yeast)

Use

Luciferase

Expression

Mammalian

Mutation

5 mutations in miR200b seed regions of 3'UTR…

Available since

May 10, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pOT_7-lenti-EFS-SS1-BBz-2A-puro

Plasmid

#217990

Purpose

Expresses anti-Mesothelin CAR (with 4-1BB signaling domain) linked to puromycin resistance via P2A for lentiviral delivery.

Depositor

Neville Sanjana

Publication

Xue et al bioRxiv [Preprint]. 2024 Nov 27:2024.11.

Insert

SS1-BBz CAR

Use

Lentiviral

Available since

Nov. 27, 2024

Availability

Academic Institutions and Nonprofits only

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α‐eGFP

Plasmid

#160214

Purpose

Retroviral construct to express an eGFP-tagged version of the α subunit of AP2

Depositor

Sandra Schmid

Publication

Mino et al Traffic. 2020 Sep;21(9):603-616. doi: 10

Insert

α‐eGFP (Ap2a2, Ap2a1 Mouse)

Use

Retroviral

Tags

eGFP at Hinge region

Mutation

Brain-specific Insert

Available since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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GRB2-V5

Plasmid

#139631

Purpose

V5-His-tagged gene expression

Depositor

Markku Varjosalo

Publication

Yadav et al iScience. 2020 Feb 21;23(2):100871. doi:

Insert

GRB2 (GRB2 Human)

Tags

3xV5-6xHis

Expression

Mammalian

Promoter

CMV

Available since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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CAPTURE2_pLVX-EF1a-NBio-dCas9-IRES-zsGreen1

Plasmid

#138419

Purpose

CAPTURE2.0 vector containing NBio-dCas9, IRES and zsGreen1

Depositor

Jian Xu

Publication

Liu et al Genome Biol. 2020 Mar 5;21(1):59. doi: 1

Insert

dCas9

Use

Lentiviral

Tags

BioTAP-tag

Promoter

EF1a

Available since

March 13, 2020

Availability

Academic Institutions and Nonprofits only

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AICSDP-28:TUBA1B-mTagRFP-T

Plasmid

#101785

Purpose

Homology arms and mTagRFPT-linker sequence for N-terminus (second exon) tagging of human TUBA1B

Depositor

Allen Institute for Cell Science

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TUBA1B Homology Arms with mTagRFPT-linker (TUBA1B Human)

Use

CRISPR; Donor template

Tags

mTagRFPT-linker

Expression

Mammalian

Mutation

homology arms contain point mutations to disrupt …

Available since

Oct. 31, 2017

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pAAV-Syn-FLEX-rc[CoChR-GFP]

Plasmid

#62724

Purpose

AAV-mediated expression of CoChR-GFP under the Syn promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.

Depositor

Edward Boyden

Publication

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

CoChR-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Promoter

human synapsin promoter

Available since

April 30, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pBabe-lincRNA-RoR

Plasmid

#45763

Depositor

George Daley

Publication

Loewer et al Nat Genet. 2010 Dec;42(12):1113-7. Epub

Insert

lincRNA-RoR cDNA (LINC-ROR Human)

Use

Retroviral

Expression

Mammalian

Available since

Aug. 12, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pFRT/FLAG/HA-DEST LIN28B

Plasmid

#43798

Depositor

Thomas Tuschl

Publication

Hafner et al RNA. 2013 May;19(5):613-26. doi: 10.1261

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

LIN28B (LIN28B Human)

Tags

FLAG-HA

Expression

Mammalian

Promoter

CMV

Available since

April 18, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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LB1353 (Bcl-2 promoter, no IgH enh)

Plasmid

#15393

Depositor

Linda Boxer

Publication

Duan et al Oncogene. 2007 Apr 19. 26(18):2635-41.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

B-cell lymphoma/leukemia-2 (BCL2 Human)

Use

Luciferase

Tags

Luciferase

Expression

Bacterial and Mammalian

Mutation

Bcl-2 with full promoter and no IgH enhancers MHS…

Available since

Aug. 1, 2007

Availability

Academic Institutions and Nonprofits only
YFP-Rac1(L61)

Plasmid

#11401

Depositor

Joel Swanson

Publication

Hoppe et al Mol Biol Cell 2004 Aug;15(8):3509-19. Ep

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ras-related C3 botulinum toxin substrate 1 (RAC1 Human)

Tags

YFP

Expression

Mammalian

Mutation

Obtained with Glutamine 61 mutated to Leucine.

Available since

Feb. 21, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pEBG-bZIPa

Plasmid

#12436

Depositor

Dong-Er Zhang

Publication

Zhang et al Mol Cell Biol. 1996 Mar . 16(3):1231-40.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

bZIP of C/EBPa (CEBPA Human)

Tags

GST

Expression

Mammalian

Mutation

pEBG vector is from Bruce Mayer, may be different…

Available since

Jan. 4, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pTREX GPR68_NB2_2

Plasmid

#127691

Purpose

Plasmid contains a nanobody that directly interacts with the cytoplasmic surface of GPR68.

Depositor

Justin English, Bryan Roth

Publication

English et al Cell. 2019 Jul 2. pii: S0092-8674(19)306

Insert

GPR68_NB2_2

Expression

Mammalian

Available since

July 2, 2019

Availability

Academic Institutions and Nonprofits only

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