We narrowed to 6,558 results for: sas;

1. DG SaCas9 GFP V3

   Plasmid

   #226961

   Purpose

   CBh-SaCas9-2A-GFP, and 2X hU6-sgRNA (Sa) with BbsI golden gate cloning backbone for dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.

   Depositor

   Paul Thomas

   Article

   Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. DG SaCas9 puro V3

   Plasmid

   #226960

   Purpose

   CBh-SaCas9-2A-Puro, and 2X hU6-sgRNA (Sa) with BbsI golden gate cloning backbone for dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.

   Depositor

   Paul Thomas

   Article

   Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEBTetDBl-CLIP-CPAP

   Plasmid

   #136865

   Purpose

   Mammalian expression of the centrosomal protein CENPJ N-terminally fused to CLIP-tag

   Depositor

   Kai Johnsson

   Article

   Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

   Insert

   CLIP-CENPJ (CENPJ Human, Synthetic)
   

   Tags

   FLAG-tag / His-tag

   Expression

   Mammalian

   Promoter

   CMV-TetO2

   Available Since

   March 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEBTetD-SNAP-CPAP

   Plasmid

   #136816

   Purpose

   Mammalian expression of the centrosomal protein CENPJ N-terminally fused to SNAP-tag

   Depositor

   Kai Johnsson

   Article

   Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

   Insert

   SNAP-CENPJ (CENPJ Human, Synthetic)
   

   Tags

   FLAG-tag / His-tag

   Expression

   Mammalian

   Promoter

   CMV-TetO2

   Available Since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pONSY-Lifeact:mCherry

   Plasmid

   #111876

   Purpose

   This plasmid expresses mCherry fused to Lifeact peptide. It labels actin bundles and filopodia in Capsaspora. Codons have been optimised according to their usages in Capsaspora and C. fragrantissima.

   Depositor

   Inaki Ruiz-Trillo

   Article

   Parra-Acero et al Development. 2018 May 11. pii: dev.16210

   Insert

   Lifeact fused to mCherry
   

   Use

   Capsaspora owczarzaki

   Tags

   mCherry

   Promoter

   Elongation Factor 1 alpha (EF1a) from Capsaspora

   Available Since

   June 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJG01

   Plasmid

   #213505

   Purpose

   expresses TdTomato in Capsaspora owczarzaki

   Depositor

   Joseph Gerdt

   Insert

   TdTomato
   

   Promoter

   EF1

   Available Since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJP118

   Plasmid

   #176494

   Purpose

   Expresses Lifeact-mScarlet under control of the Capsaspora EF1 promoter and HygR under control of the Capsaspora actin promoter

   Depositor

   Duojia Pan

   Article

   Phillips et al Elife. 2022 Jun 6;11. pii: 77598. doi: 1

   Insert

   Lifeact peptide
   

   Use

   Capsaspora owczarzaki expression

   Available Since

   June 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pONSY

   Plasmid

   #111873

   Purpose

   "Capsaspora owczarzaki" expression vector (backbone) modified from the pCR2.1. It bears the promoter and terminator regions from the endogenous Elongation Factor 1 alpha (EF1a) gene (CAOG_07807).

   Depositor

   Inaki Ruiz-Trillo

   Article

   Parra-Acero et al Development. 2018 May 11. pii: dev.16210

   Type

   Empty backbone

   Use

   Capsaspora owczarzaki

   Promoter

   Elongation Factor 1 alpha (EF1a) from Capsaspora

   Available Since

   July 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. ER-NFAST

   Plasmid

   #233596

   Purpose

   Expression of NFAST on the ER membrane

   Depositor

   Riccardo Filadi

   Article

   Garcia Casas et al Nat Commun. 2024 Nov 12;15(1):9775. doi:

   Insert

   NFAST
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pONSY-mCherry

    Plasmid

    #111874

    Purpose

    This plasmid expresses mCherry fluorescent protein under endogenous EF1a promoter. It can be used to transfect "Capsaspora owczarzaki" cells and visualize them by fluorescence microscopy.

    Depositor

    Inaki Ruiz-Trillo

    Article

    Parra-Acero et al Development. 2018 May 11. pii: dev.16210

    Insert

    mCherry
    

    Use

    Capsaspora owczarzaki

    Tags

    mCherry

    Promoter

    Elongation Factor 1 alpha (EF1a) from Capsaspora

    Available Since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pONSY-Venus

    Plasmid

    #111875

    Purpose

    This plasmid expresses Venus fluorescent protein under endogenous EF1a promoter. It can be used to transfect "Capsaspora owczarzaki" cells and visualize them by fluorescence microscopy.

    Depositor

    Inaki Ruiz-Trillo

    Article

    Parra-Acero et al Development. 2018 May 11. pii: dev.16210

    Insert

    Venus
    

    Use

    Capsaspora owczarzaki

    Tags

    Venus

    Promoter

    Elongation Factor 1 alpha (EF1a) from Capsaspora

    Available Since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. ER-frNFAST

    Plasmid

    #233599

    Purpose

    Expression of frNFAST on the ER membrane

    Depositor

    Riccardo Filadi

    Article

    Garcia Casas et al Nat Commun. 2024 Nov 12;15(1):9775. doi:

    Insert

    frNFAST
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. PM-frNFAST

    Plasmid

    #233602

    Purpose

    Expression of frNFAST on the PM facing the cytosol

    Depositor

    Riccardo Filadi

    Article

    Garcia Casas et al Nat Commun. 2024 Nov 12;15(1):9775. doi:

    Insert

    frNFAST
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. PM-RspA-CFAST

    Plasmid

    #233601

    Purpose

    Expression of RspA-CFAST on the PM facing the cytosol

    Depositor

    Riccardo Filadi

    Article

    Garcia Casas et al Nat Commun. 2024 Nov 12;15(1):9775. doi:

    Insert

    RspA-CFAST
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. OMM-long-CFAST10

    Plasmid

    #233598

    Purpose

    Expression of CFAST10 on the OMM membrane after a long linker

    Depositor

    Riccardo Filadi

    Article

    Garcia Casas et al Nat Commun. 2024 Nov 12;15(1):9775. doi:

    Insert

    CFAST10
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. OMM-short-CFAST10

    Plasmid

    #233597

    Purpose

    Expression of CFAST10 on the OMM membrane after a short linker

    Depositor

    Riccardo Filadi

    Article

    Garcia Casas et al Nat Commun. 2024 Nov 12;15(1):9775. doi:

    Insert

    CFAST10
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. ER-RspA-CFAST

    Plasmid

    #233607

    Purpose

    Expression of RspA-CFAST on the ER membrane

    Depositor

    Riccardo Filadi

    Article

    Garcia Casas et al Nat Commun. 2024 Nov 12;15(1):9775. doi:

    Insert

    RspA-CFAST
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. OMM-short-RspA-NFAST

    Plasmid

    #233603

    Purpose

    Expression of RspA-NFAST on the outer mitochondrial membrane

    Depositor

    Riccardo Filadi

    Article

    Garcia Casas et al Nat Commun. 2024 Nov 12;15(1):9775. doi:

    Insert

    RspA-NFAST
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. ER-RspA-NFAST

    Plasmid

    #233595

    Purpose

    Expression of RspA-NFAST on the ER membrane

    Depositor

    Riccardo Filadi

    Article

    Garcia Casas et al Nat Commun. 2024 Nov 12;15(1):9775. doi:

    Insert

    RspA-NFAST
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. ER-long-RspA-NFAST

    Plasmid

    #233600

    Purpose

    Expression of RspA-NFAST on the ER membrane after a 55 aminoacid linker

    Depositor

    Riccardo Filadi

    Article

    Garcia Casas et al Nat Commun. 2024 Nov 12;15(1):9775. doi:

    Insert

    RspA-NFAST
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge