We narrowed to 317 results for: 10B

1. pAS_4xEcoLeuT(CUA)_EF1_AnapRS

   Plasmid

   #174894

   Purpose

   AnapRS expression for amber suppression in mammalian cells; transient or piggy bac mediated integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

   Insert

   AnapRS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   L38F, M40G, L41P, Y499V, Y500L, Y527A, H537E, L53…

   Promoter

   EF1

   Available since

   Nov. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. eGFP-KIF5A-Rigor

   Plasmid

   #172202

   Purpose

   Expresses KIF5A G235A rigor mutant tagged with eGFP in mammalian cells

   Depositor

   Juan Bonifacino

   Article

   Farias et al Cell Rep. 2015 Nov 10;13(6):1221-1232. d

   Insert

   KIF5A (Kif5a Mouse)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Changed Glycine 235 to Alanine

   Promoter

   CMV

   Available since

   July 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDEST47-ARL8A

   Plasmid

   #67466

   Purpose

   Mammalian expression of hArl8 with stop codon (native expression)

   Depositor

   Richard Kahn

   Article

   Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

   Insert

   ARL8A (ARL8A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pDEST40-ARL8A-V5-His6

   Plasmid

   #67445

   Purpose

   Mammalian expression of hArl8 with C-term V5 and His6 tag

   Depositor

   Richard Kahn

   Article

   Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

   Insert

   ARL8A (ARL8A Human)
   

   Use

   Tags

   His and V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pACYC-mTET1-N

   Plasmid

   #81056

   Purpose

   bacterial expression of N-terminus of murine TET1

   Depositor

   Primo Schaer

   Article

   Weber et al Nat Commun. 2016 Mar 2;7:10806. doi: 10.

   Insert

   TET1 (Tet1 Mouse)
   

   Use

   Tags

   6HIS

   Expression

   Bacterial

   Mutation

   N-terminus, amino acids 301-1366

   Promoter

   T7

   Available since

   Aug. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDONR221-ARL8A-no stop

   Plasmid

   #67333

   Purpose

   hArl8a without stop codon in Gateway Entry vector

   Depositor

   Richard Kahn

   Article

   Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

   Insert

   ARL8A (ARL8A Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   None

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

7. CLIPf-KIF5A(1-914; Δ505-610)-SNAPf-HisTag-FLAG

   Plasmid

   #200793

   Purpose

   Mammalian expression of CLIPf-KIF5A(1-914; Δ505-610)-SNAPf-HisTag-FLAG

   Depositor

   Alison Twelvetrees

   Article

   Smith et al bioRxiv 2024.12.20.629623

   Insert

   KIF5A (Kif5a Mouse)
   

   Use

   Tags

   CLIPf and SNAPf, HisTag, FLAG

   Expression

   Mammalian

   Mutation

   1-914; Δ505-610

   Promoter

   CMV & T7

   Available since

   Dec. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAS_4xKBstTyrT(CUA)_EF1_sfGFP 102TAG 150TAA

   Plasmid

   #174895

   Purpose

   amber and ochre dual suppression reporter sfGFP 102TAG 150 TAA expression, with BstTyr(CUA) amber suppressor tRNA cassette

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

   Insert

   sfGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   102TAG 150TAA in sfGFP

   Promoter

   EF1

   Available since

   Nov. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET-DEST42-ARL8A-V5-His6

   Plasmid

   #67424

   Purpose

   Expression of hArl8 with C-term V5 and His6 tag in E. coli

   Depositor

   Richard Kahn

   Article

   Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

   Insert

   ARL8A (ARL8A Human)
   

   Use

   Tags

   His and V5

   Expression

   Bacterial

   Mutation

   Promoter

   T7/lacO

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pPB-Ins-U6p-sgRNAentry-EF1Ap-TetOn3G-IRES-Neo

    Plasmid

    #183411

    Purpose

    piggyBAC-based sgRNA entry (Esp3I) and Tet-On 3G transactivator expression vector. Insert protospacer and transfect with CRISPRa (183409) or CRISPRi (183410) plasmid for inducible epigenome editing.

    Depositor

    Azim Surani

    Article

    Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d

    Insert

    Tet-on 3G
    

    Use

    CRISPR and Synthetic Biology; Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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