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We narrowed to 29,083 results for: Tat

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Showing: 841 - 880 of 29083 results

  4. pcDNA3.1-IARS2-H112A/P115A

    Plasmid
    #232338
    Purpose
    Expresses human IARS2 with putative isoleucine binding deficiency mutations (H112A and P115A) in mammalian cells, also carrying synonymous mutations described in Addgene #232340
    Depositor
    Quanfu Ma
    Insert
    IARS2 (IARS2 Human)
    Expression
    Mammalian
    Mutation
    Putative isoleucine binding deficiency mutation H…
    Promoter
    CMV
    Available Since
    June 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    232338-plasmid-map-sequence-id-474537

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  5. pcDNA3.1-IARS2-K664A/K667A

    Plasmid
    #232339
    Purpose
    Expresses human IARS2 with putative tRNA binding deficiency mutations (K664A and K667A) in mammalian cells, also carrying synonymous mutations described in Addgene #232340
    Depositor
    Quanfu Ma
    Insert
    IARS2 (IARS2 Human)
    Expression
    Mammalian
    Mutation
    Putative tRNA binding deficiency mutation K664A A…
    Promoter
    CMV
    Available Since
    June 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    232339-plasmid-map-sequence-id-474533

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  6. pCEP4-CH111-QES.c13.L544Y-754*

    Plasmid
    #123264
    Purpose
    Mammalian expression plasmid for Env from the CH111 HIV-1 isolate; C-terminal truncation; QES mutant for enhanced presentation of quaternary epitopes
    Depositor
    Erik Procko
    Article
    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.
    Insert
    HIV-1 (AD8) Env
    Tags
    CD5 leader peptide
    Expression
    Mammalian
    Mutation
    Codon-optimized synthetic gene; Premature stop co…
    Promoter
    CMV
    Available Since
    April 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    123264-plasmid-map-sequence-id-235227

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  7. pCEP4-AD8-QES.i08.c04-754*

    Plasmid
    #123258
    Purpose
    Mammalian expression plasmid for Env from the AD8 HIV-1 isolate; C-terminal truncation; QES mutant for enhanced presentation of quaternary epitopes
    Depositor
    Erik Procko
    Article
    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.
    Insert
    HIV-1 (AD8) Env
    Tags
    CD5 leader peptide
    Expression
    Mammalian
    Mutation
    Codon-optimized synthetic gene; Premature stop co…
    Promoter
    CMV
    Available Since
    April 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    123258-plasmid-map-sequence-id-235223

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  8. pCEP4-SHIV327C-HuA10-QES.i09.c07

    Plasmid
    #123269
    Purpose
    Mammalian expression plasmid for Env from the SHIV327C-Hu A10 strain; QES mutant for enhanced presentation of quaternary epitopes
    Depositor
    Erik Procko
    Article
    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.
    Insert
    SHIV327C-Hu A10 Env
    Tags
    CD5 leader peptide
    Expression
    Mammalian
    Mutation
    Codon-optimized synthetic gene; Mutations V208M, …
    Promoter
    CMV
    Available Since
    March 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    123269-plasmid-map-sequence-id-235232

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  10. pET30a-His6-ABP-LOXCAT

    Plasmid
    #139971
    Purpose
    Fusion of A. viridans lactate oxidase (LOX) and E. coli catalase (CAT) that converts lactate into pyruvate without producing H2O2. Contains a albumin binding peptide (ABP).
    Depositor
    Vamsi Mootha
    Article
    Patgiri et al Nat Biotechnol. 2020 Jan 13. pii: 10.103
    Insert
    A fusion of lactate oxidase from A. viridans and catalase from E. coli
    Tags
    His6
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Sept. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    139971-plasmid-map-sequence-id-311161

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  11. pET30a-His6-ABP-LOXCATmut

    Plasmid
    #139972
    Purpose
    A control for His6-ABP-LOXCAT where LOX is catalytically dead (mutations H265A and R268A)
    Depositor
    Vamsi Mootha
    Article
    Patgiri et al Nat Biotechnol. 2020 Jan 13. pii: 10.103
    Insert
    A fusion of lactate oxidase from A. viridans and catalase from E. coli
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Mutation in H265A and R268A in LOX (used original…
    Promoter
    T7
    Available Since
    Sept. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    139972-plasmid-map-sequence-id-311162

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  12. TFORF3173

    Plasmid
    #144649
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    STAT6 (STAT6 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    144649-plasmid-map-sequence-id-348625

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  13. TFORF3172

    Plasmid
    #144648
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    STAT6 (STAT6 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    144648-plasmid-map-sequence-id-348623

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  14. pYihI-N-term D-N/E-Q

    Plasmid
    #233095
    Purpose
    Expression of GST-YihI with N-term aspartic acids (D) mutated to asparagine (N) and glutamic acids (E) mutated to glutamine (Q)
    Depositor
    Michael Downey
    Article
    Baijal et al mBio. 2025 Aug 13;16(8):e0050025. doi: 1
    Insert
    YihI with N-term aspartic acids (D) mutated to asparagine (N) and glutamic acids (E) mutated to glutamine (Q)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    N-term aspartic acids (D) mutated to asparagine (…
    Available Since
    May 7, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    233095-plasmid-map-sequence-id-468389

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  15. pYihI-N-term D-A/E-L

    Plasmid
    #233097
    Purpose
    Expression of GST-YihI with N-term aspartic acids (D) mutated to alanine (A) and glutamic acids (E) mutated to leucine (L)
    Depositor
    Michael Downey
    Article
    Baijal et al mBio. 2025 Aug 13;16(8):e0050025. doi: 1
    Insert
    YihI with N-term aspartic acids (D) mutated to alanine (A) and glutamic acids (E) mutated to leucine (L)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    N-term aspartic acids (D) mutated to alanine (A) …
    Available Since
    May 7, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    233097-plasmid-map-sequence-id-468390

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  18. pCEP4-BG505.SOSIP-QES.i03.c01-8his

    Plasmid
    #111846
    Purpose
    Mammalian expression plasmid for soluble BG505 SOSIP.664; QES mutant with enhanced binding to PG16
    Depositor
    Erik Procko
    Article
    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.
    Insert
    HIV-1 Env (BG505 SOSIP.664)
    Tags
    8his purification tag and CD5 leader sequence
    Expression
    Mammalian
    Mutation
    Codon-optimized synthetic gene; has SOSIP mutatio…
    Promoter
    CMV
    Available Since
    March 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    111846-plasmid-map-sequence-id-210258

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  21. pCEP4-BG505.SOSIP-QES.i03.c01-TM

    Plasmid
    #111845
    Purpose
    Mammalian expression plasmid for BG505 SOSIP.664 fused to a transmembrane tether; QES mutant with enhanced binding to PG16
    Depositor
    Erik Procko
    Article
    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.
    Insert
    HIV-1 Env (BG505 SOSIP.664)
    Tags
    CD5 leader sequence and TM helix from MHC class I
    Expression
    Mammalian
    Mutation
    Codon-optimized synthetic gene; has SOSIP mutatio…
    Promoter
    CMV
    Available Since
    March 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    111845-plasmid-map-sequence-id-210256

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  24. pCW57-GFP-P2A-EcASPase

    Plasmid
    #252079
    Purpose
    All-in-one doxycycline inducible lentiviral vector for expression of E.coli derived aspartate lyase in combination with turbo GFP using the P2A self-cleaving peptide.
    Depositor
    Lydia Finley
    Article
    Brunner et al Mol Cell. 2026 Feb 26:S1097-2765(26)0009
    Insert
    Codon optimized E.coli derived aspartate ammonia-lyase
    Use
    Lentiviral; Doxycycline inducible; p2a self cleav…
    Expression
    Mammalian
    Mutation
    Codon optimized
    Promoter
    TRE
    Available Since
    March 5, 2026
    Availability
    Academic Institutions and Nonprofits only
    252079-plasmid-map-sequence-id-501997

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  25. pCW57-GFP-P2A-ByASPase

    Plasmid
    #252094
    Purpose
    All-in-one doxycycline inducible lentiviral vector for expression of Bacillus sp. YM55-1 derived aspartate lyase in combination with turbo GFP using the P2A self-cleaving peptide.
    Depositor
    Lydia Finley
    Article
    Brunner et al Mol Cell. 2026 Feb 26:S1097-2765(26)0009
    Insert
    Codon optimized Bacillus sp. YM55-1 derived aspartate ammonia-lyase
    Use
    Lentiviral; Doxycycline inducible; p2a self cleav…
    Expression
    Mammalian
    Mutation
    Codon optimized
    Promoter
    TRE
    Available Since
    Feb. 26, 2026
    Availability
    Academic Institutions and Nonprofits only
    252094-plasmid-map-sequence-id-501215

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  29. DBDmut-CA-RIT-NFAT1

    Plasmid
    #63669
    Purpose
    Constitutively active NFAT1 and also unable to interact with AP-1 with four point mutations in the DNA binding loop that abolish DNA binding
    Depositor
    Anjana Rao
    Article
    Martinez et al Immunity. 2015 Feb 6. pii: S1074-7613(15
    Insert
    NFAT1 (Nfatc2 Mouse)
    Use
    Retroviral
    Tags
    3x HA
    Expression
    Mammalian
    Mutation
    CA: PASSGSSASF mutated to PAAAGAAAAF, SPRTSPIMSP…
    Available Since
    July 22, 2015
    Availability
    Academic Institutions and Nonprofits only

  31. pLY54

    Plasmid
    #130923
    Purpose
    A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA2B2 with sfgfp::ASV).
    Depositor
    Baojun Wang
    Article
    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:
    Inserts
    dcas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    Use
    Synthetic Biology
    Tags
    ASV tag and pspFΔHTH::λN22plus
    Expression
    Bacterial
    Mutation
    Mutations D10A and H840A on WT cas9. Synonymous m…
    Promoter
    Anderson promoter: J23106, PpspA-LEA2B2, and Ptet
    Available Since
    Sept. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    130923-plasmid-map-sequence-id-257307

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  33. pLY9

    Plasmid
    #130907
    Purpose
    A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-2G6 with sfgfp::ASV).
    Depositor
    Baojun Wang
    Article
    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:
    Inserts
    dcas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    Use
    Synthetic Biology
    Tags
    ASV tag and pspFΔHTH::λN22plus
    Mutation
    Mutations D10A and H840A on WT cas9. Synonymous m…
    Promoter
    Anderson promoter: J23106, PpspA-2G6, and Ptet
    Available Since
    Oct. 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    130907-plasmid-map-sequence-id-258306

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  34. pLY59

    Plasmid
    #130927
    Purpose
    A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::MCP controlled by promoter J23106), and the reporter part (PpspA-2G6 with sfgfp::ASV).
    Depositor
    Baojun Wang
    Article
    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:
    Inserts
    dcas9
    tetR
    sfgfp
    pspFΔHTH::MCP
    Use
    Synthetic Biology
    Tags
    ASV tag and MCP (MS2 coat protein)
    Expression
    Bacterial
    Mutation
    Mutations D10A and H840A on WT cas9. Synonymous m…
    Promoter
    Anderson promoter: J23106, PpspA-2G6, and Ptet
    Available Since
    Sept. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    130927-plasmid-map-sequence-id-257328

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  35. pLY57

    Plasmid
    #130926
    Purpose
    A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA5B5 with sfgfp::ASV).
    Depositor
    Baojun Wang
    Article
    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:
    Inserts
    dcas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    Use
    Synthetic Biology
    Tags
    ASV tag and pspFΔHTH::λN22plus
    Expression
    Bacterial
    Mutation
    Mutations D10A and H840A on WT cas9. Synonymous m…
    Promoter
    Anderson promoter: J23106, PpspA-LEA5B5, and Ptet
    Available Since
    Sept. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    130926-plasmid-map-sequence-id-257327

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  36. pLY56

    Plasmid
    #130925
    Purpose
    A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA4B4 with sfgfp::ASV).
    Depositor
    Baojun Wang
    Article
    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:
    Inserts
    dcas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    Use
    Synthetic Biology
    Tags
    ASV tag and pspFΔHTH::λN22plus
    Expression
    Bacterial
    Mutation
    Mutations D10A and H840A on WT cas9. Synonymous m…
    Promoter
    Anderson promoter: J23106, PpspA-LEA4B4, and Ptet
    Available Since
    Sept. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    130925-plasmid-map-sequence-id-257326

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  37. pLY55

    Plasmid
    #130924
    Purpose
    A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA3B3 with sfgfp::ASV).
    Depositor
    Baojun Wang
    Article
    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:
    Inserts
    dcas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    Use
    Synthetic Biology
    Tags
    ASV tag and pspFΔHTH::λN22plus
    Expression
    Bacterial
    Mutation
    Mutations D10A and H840A on WT cas9. Synonymous m…
    Promoter
    Anderson promoter: J23106, PpspA-LEA3B3, and Ptet
    Available Since
    Sept. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    130924-plasmid-map-sequence-id-257309

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  38. pLY53

    Plasmid
    #130911
    Purpose
    A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA1B1 with sfgfp::ASV).
    Depositor
    Baojun Wang
    Article
    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:
    Inserts
    dcas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    Use
    Synthetic Biology
    Tags
    ASV tag and pspFΔHTH::λN22plus
    Expression
    Bacterial
    Mutation
    Mutations D10A and H840A on WT cas9. Synonymous m…
    Promoter
    Anderson promoter: J23106, PpspA-LEA1B1, and Ptet
    Available Since
    Sept. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    130911-plasmid-map-sequence-id-257304

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  39. AAV-KrasG13D/sgKras/Cre

    Plasmid
    #99857
    Purpose
    Expresses Cre-recombinase, a barcoded HDR template to introduce a G13D mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.
    Depositor
    Monte Winslow
    Article
    Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:
    Insert
    Kras (Kras Mouse)
    Use
    AAV and Cre/Lox
    Expression
    Mammalian
    Mutation
    AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…
    Available Since
    Dec. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    99857-plasmid-map-sequence-id-193798

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  40. AAV-KrasG12D/sgKras/Cre

    Plasmid
    #99851
    Purpose
    Expresses Cre-recombinase, a barcoded HDR template to introduce a G12D mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.
    Depositor
    Monte Winslow
    Article
    Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:
    Insert
    Kras (Kras Mouse)
    Use
    AAV and Cre/Lox
    Expression
    Mammalian
    Mutation
    AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…
    Available Since
    Dec. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    99851-plasmid-map-sequence-id-193760

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Showing: 841 - 880 of 29083 results