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We narrowed to 5,049 results for: golden gate

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Showing: 1051 - 1100 of 5049 results

  1. SP_A3

    Plasmid
    #228147
    Purpose
    Plasmid contains the secretion signal peptide sequence of the human ECM33 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).
    Depositor
    Joseph Brock
    Article
    Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86
    Insert
    sp_P38248_ECM33_YEAST:0-19
    Use
    Synthetic Biology
    Available Since
    July 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    228147-plasmid-map-sequence-id-451649

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  2. SP_A4

    Plasmid
    #228148
    Purpose
    Plasmid contains the secretion signal peptide sequence of the human CHADL gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).
    Depositor
    Joseph Brock
    Article
    Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86
    Insert
    sp_Q6NUI6_CHADL_HUMAN:0-30
    Use
    Synthetic Biology
    Available Since
    July 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    228148-plasmid-map-sequence-id-451693

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  3. SP_A5

    Plasmid
    #228149
    Purpose
    Plasmid contains the secretion signal peptide sequence of the human GDF8 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).
    Depositor
    Joseph Brock
    Article
    Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86
    Insert
    sp_O14793_GDF8_HUMAN:0-18
    Use
    Synthetic Biology
    Available Since
    July 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    228149-plasmid-map-sequence-id-451779

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  4. pKEK3236

    Plasmid
    #234108
    Purpose
    CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.
    Depositor
    Karl Klose
    Article
    Armstrong et al Appl Environ Microbiol. 2025 May 12:e000
    Insert
    sfCherry2
    Expression
    Bacterial
    Mutation
    E117Q/T127I/G219A
    Promoter
    pJ23100-BsaI
    Available Since
    April 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    234108-plasmid-map-sequence-id-467997

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  5. pKEK3221

    Plasmid
    #234100
    Purpose
    CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.
    Depositor
    Karl Klose
    Article
    Armstrong et al Appl Environ Microbiol. 2025 May 12:e000
    Insert
    sfcherry 2
    Expression
    Bacterial
    Mutation
    E117Q/T127I/G219A
    Promoter
    pJ23100-BsaI
    Available Since
    April 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    234100-plasmid-map-sequence-id-468004

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  6. pKEK3237

    Plasmid
    #234091
    Purpose
    CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.
    Depositor
    Karl Klose
    Article
    Armstrong et al Appl Environ Microbiol. 2025 May 12:e000
    Insert
    sfGFP
    Tags
    FLAG
    Expression
    Bacterial
    Mutation
    S30R/Y39N/N105T/Y145F/I171V/A206V
    Promoter
    pJ23100-BsaI
    Available Since
    April 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    234091-plasmid-map-sequence-id-467987

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  7. pKEK3397

    Plasmid
    #234081
    Purpose
    CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.
    Depositor
    Karl Klose
    Article
    Armstrong et al Appl Environ Microbiol. 2025 May 12:e000
    Insert
    FuGFP
    Expression
    Bacterial
    Promoter
    pJ23100-BsaI
    Available Since
    April 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    234081-plasmid-map-sequence-id-468005

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  8. pKEK3224

    Plasmid
    #234086
    Purpose
    CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.
    Depositor
    Karl Klose
    Article
    Armstrong et al Appl Environ Microbiol. 2025 May 12:e000
    Insert
    FuGFP
    Expression
    Bacterial
    Promoter
    pJ23100-BsaI
    Available Since
    April 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    234086-plasmid-map-sequence-id-468001

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  9. pKEK3197

    Plasmid
    #234095
    Purpose
    CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.
    Depositor
    Karl Klose
    Article
    Armstrong et al Appl Environ Microbiol. 2025 May 12:e000
    Insert
    YukonOFP
    Expression
    Bacterial
    Promoter
    pJ23100-BsaI
    Available Since
    March 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    234095-plasmid-map-sequence-id-464928

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  10. pKEK3199

    Plasmid
    #234097
    Purpose
    CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.
    Depositor
    Karl Klose
    Article
    Armstrong et al Appl Environ Microbiol. 2025 May 12:e000
    Insert
    tspurple
    Expression
    Bacterial
    Promoter
    pJ23100-BsaI
    Available Since
    March 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    234097-plasmid-map-sequence-id-464933

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  11. pKEK3200

    Plasmid
    #234098
    Purpose
    CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.
    Depositor
    Karl Klose
    Article
    Armstrong et al Appl Environ Microbiol. 2025 May 12:e000
    Insert
    fuGFP
    Expression
    Bacterial
    Promoter
    pJ23100-BsaI
    Available Since
    March 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    234098-plasmid-map-sequence-id-464935

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  12. pKEK3232

    Plasmid
    #234104
    Purpose
    CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.
    Depositor
    Karl Klose
    Article
    Armstrong et al Appl Environ Microbiol. 2025 May 12:e000
    Insert
    tsPurple
    Expression
    Bacterial
    Promoter
    pJ23100-BsaI
    Available Since
    March 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    234104-plasmid-map-sequence-id-464926

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  13. pKEK3400

    Plasmid
    #234084
    Purpose
    CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.
    Depositor
    Karl Klose
    Article
    Armstrong et al Appl Environ Microbiol. 2025 May 12:e000
    Insert
    sfGFP
    Tags
    FLAG
    Expression
    Bacterial
    Mutation
    S30R/Y39N/N105T/Y145F/I171V/A206V
    Promoter
    pJ23100-BsaI
    Available Since
    March 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    234084-plasmid-map-sequence-id-464936

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  36. pINEAPLE

    Plasmid
    #250295
    Purpose
    Big-IN Payload assembly vector by Golden Gate Cloning using Esp3I. Contains a bacterial green/white GFP-based screening system and a mammalian transient (backbone) BSD selection cassette.
    Depositor
    Matthew Maurano
    Article
    Ordoñez et al bioRxiv 2025.08.13.669251
    Insert
    SuperfolderGFP
    Use
    Synthetic Biology; Assembly vector for big-in pay…
    Available Since
    Feb. 3, 2026
    Availability
    Academic Institutions and Nonprofits only
    250295-plasmid-map-sequence-id-498935

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  37. pCASB

    Plasmid
    #190175
    Purpose
    Plasmid for yeast expression of Cas9; contains a golden gate cloning site to additionally express a gRNA for a target sequence
    Depositor
    Jessica Tyler
    Article
    Aguilar et al Methods Protoc. 2022 Oct 4;5(5):79. doi:
    Insert
    sgRNA cloning site
    Expression
    Bacterial and Yeast
    Mutation
    Added 5'-agagacc-3' upstream and 5'…
    Available Since
    Oct. 5, 2022
    Availability
    Academic Institutions and Nonprofits only
    190175-plasmid-map-sequence-id-372326

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  38. pp-CBBC-Fluc

    Plasmid
    #232920
    Purpose
    Allows the Golden Gate (BsmBI) cloning of pegRNA between two Csy4-hairpins in the intron of a CMV-FLUC encoding plasmid (POL II): ipegRNAs must be processed by Csy4 in VLP- producer cells.
    Depositor
    Philippe Mangeot
    Article
    Halegua et al Nat Commun. 2025 Jan 4;16(1):397. doi: 1
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    232920-plasmid-map-sequence-id-464557

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  42. pGA-blue

    Plasmid
    #196336
    Purpose
    Easy-MISE toolkit Level 0 destination plasmid for parts cloning with Esp3I-based Golden Gate Assembly; allowing fast postitive clones test with AmilCP chromoprotein-based blue/white screening
    Depositor
    Paola Branduardi
    Article
    Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102
    Type
    Empty backbone
    Use
    Synthetic Biology; Golden gate assembly backbone
    Available Since
    May 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    196336-plasmid-map-sequence-id-393157

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  45. pKEK3235

    Plasmid
    #234107
    Purpose
    CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.
    Depositor
    Karl Klose
    Article
    Armstrong et al Appl Environ Microbiol. 2025 May 12:e000
    Insert
    sfGFP
    Tags
    FLAG
    Expression
    Bacterial
    Mutation
    S30R/Y39N/N105T/Y145F/I171V/A206V
    Promoter
    pJ23100-BsaI
    Available Since
    April 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    234107-plasmid-map-sequence-id-467990

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Showing: 1051 - 1100 of 5049 results