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We narrowed to 4,045 results for: golden gate

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Showing: 1051 - 1100 of 4045 results

  1. C76m

    Plasmid
    #120988
    Purpose
    MoClo golden gate assembly CD part for KanR (kanamycin resistance protein). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    KanR
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120988-plasmid-map-sequence-id-236053

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  2. PACE_AP_GIII_Titrate

    Plasmid
    #242140
    Purpose
    PSP-LacO Cassette; AP Prop Circuit for AraC phage-assisted evolution
    Depositor
    Dominik Niopek
    Insert
    Gene III
    Expression
    Bacterial
    Available Since
    Aug. 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    242140-plasmid-map-sequence-id-484675

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  3. pBD_PYR1_156-F1

    Plasmid
    #241286
    Purpose
    Backbone for PYR1 sensor library assembly from oligopools; stop codon removed; PYR1 sensor library assembly from oligopools;
    Depositor
    Sean Cutler
    Type
    Empty backbone
    Expression
    Yeast
    Available Since
    Aug. 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    241286-plasmid-map-sequence-id-484590

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  4. pKS1469

    Plasmid
    #239517
    Purpose
    Express the upper portion of the melvaonate pathway enzymes (MvaE and MvaS) in the peroxisome
    Depositor
    John Dueber
    Insert
    EfMvaE-PTS1
    Expression
    Yeast
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239517-plasmid-map-sequence-id-475898

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  5. pAIP4_1EDG

    Plasmid
    #234144
    Purpose
    Heterologous protein expression of GUNA_RUMCH in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    1EDG
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234144-plasmid-map-sequence-id-464110

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  6. U4m

    Plasmid
    #121017
    Purpose
    MoClo golden gate assembly BC part for UTR1 (very strong RBS). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    UTR1
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121017-plasmid-map-sequence-id-236179

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  7. cBEST4

    Plasmid
    #234660
    Purpose
    Expresses cytosine base editor - spCas9n (D10A) fused to APOBEC1 and UGI, Include Golden Gate compatible cassette for sgRNA insertion
    Depositor
    Mingzi M. Zhang
    Article
    Lin et al Appl Environ Microbiol. 2025 Mar 10:e019
    Inserts
    spCas9 cytosine base editor
    Golden Gate compatible sgRNA insertion cassette
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    P3 and tcp830
    Available Since
    May 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    234660-plasmid-map-sequence-id-472864

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  10. pAV10.F3

    Plasmid
    #63199
    Purpose
    BioBrick pSB1C3 backbone (Cm), HIS3, RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. Integration into chrVI: 97873-98803 (NotI or BciVI).
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Available Since
    Nov. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    63199-plasmid-map-sequence-id-108468

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  11. pAV10.KH

    Plasmid
    #63208
    Purpose
    pCACS backbone (Amp), Hyg, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into YKL162C-A (NotI).
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Available Since
    Sept. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    63208-plasmid-map-sequence-id-104909

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  12. pAV10.KN

    Plasmid
    #63209
    Purpose
    pCACS backbone (Amp), cloNAT, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into YKL162C-A (NotI).
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Available Since
    April 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    63209-plasmid-map-sequence-id-115799

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  15. pAV10.K5

    Plasmid
    #63210
    Purpose
    pCACS backbone (Amp), LEU2, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into YKL162C-A (NotI or BciVI).
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Available Since
    Feb. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    63210-plasmid-map-sequence-id-104911

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  16. pAV10.K4

    Plasmid
    #63212
    Purpose
    pCACS backbone (Amp), TRP1, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into chrIXR: 387328-388330.
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Available Since
    Feb. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    63212-plasmid-map-sequence-id-104913

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  22. pAV113.loxP

    Plasmid
    #63186
    Purpose
    pRS413 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. TU is also flanked by LoxP sites.
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Available Since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    63186-plasmid-map-sequence-id-131462

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  23. pAV115.loxP

    Plasmid
    #63187
    Purpose
    pRS415 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. TU is also flanked by LoxP sites.
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Available Since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    63187-plasmid-map-sequence-id-104891

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  24. pAV116.loxP

    Plasmid
    #63188
    Purpose
    pRS416 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. TU is also flanked by LoxP sites.
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Available Since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    63188-plasmid-map-sequence-id-121508

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  25. cBEST2

    Plasmid
    #234658
    Purpose
    Expresses cytosine base editor - spCas9n (D10A) fused to APOBEC1 and UGI, Include Golden Gate compatible cassette for sgRNA insertion
    Depositor
    Mingzi M. Zhang
    Article
    Lin et al Appl Environ Microbiol. 2025 Mar 10:e019
    Inserts
    spCas9 cytosine base editor
    Golden Gate compatible sgRNA insertion cassette
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    kasO*17tss and kasOp*
    Available Since
    Nov. 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    234658-plasmid-map-sequence-id-492748

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  26. cBEST3

    Plasmid
    #234659
    Purpose
    Expresses cytosine base editor - spCas9n (D10A) fused to APOBEC1 and UGI, Include Golden Gate compatible cassette for sgRNA insertion
    Depositor
    Mingzi M. Zhang
    Article
    Lin et al Appl Environ Microbiol. 2025 Mar 10:e019
    Inserts
    spCas9 cytosine base editor
    Golden Gate compatible sgRNA insertion cassette
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    P3 and kasO17tss
    Available Since
    Nov. 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    234659-plasmid-map-sequence-id-492747

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  49. pAV10.K3.loxP

    Plasmid
    #63202
    Purpose
    pCACS backbone (Amp), SpHIS5, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into YKL162C-A (NotI).
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Availability
    Academic Institutions and Nonprofits only
    63202-plasmid-map-sequence-id-104899

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  50. pAV10.K5.loxP

    Plasmid
    #63204
    Purpose
    pCACS backbone (Amp), LEU2, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into YKL162C-A (NotI or BciVI).
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Availability
    Academic Institutions and Nonprofits only
    63204-plasmid-map-sequence-id-104903

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Showing: 1051 - 1100 of 4045 results