Addgene

We narrowed to 4,183 results for: 256

Showing: 421 - 440 of 4183 results

Results per page:

Sort results by:

pBS-KS-attB1-2-PT-SA-SD-1-2xTY1-V5

Plasmid

#61256

Purpose

CRISPR-RMCE, second step attB plasmid, intron phase 1, exchange dsRed with 2xTY1 and V5 tags cassette

Depositor

Frank Schnorrer

Article

Zhang et al G3 (Bethesda). 2014 Oct 15. pii: g3.114.

Insert

2xTY1-V5

Use

CRISPR

Tags

TY1 and V5

Expression

Mutation

Promoter

Available since

Nov. 20, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pJKR-L-ttgR

Plasmid

#62566

Purpose

Flavonoid biosensor. Low copy plasmid that expresses GFP proportional to flavonoid concentration.

Depositor

George Church

Article

Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

Insert

GFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pFF758

Plasmid

#61454

Purpose

IPTG-inducible msd(wt)_dRT

Depositor

Timothy Lu

Article

Farzadfard et al Science. 2014 Nov 14;346(6211):1256272.

Insert

msd(wt)_dRT

Use

Synthetic Biology

Tags

Expression

Mutation

D197A D198A

Promoter

pLacO

Available since

Feb. 20, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
YIplac211-CHC1-msGFPx3

Plasmid

#105256

Purpose

Plasmid for pop-in/pop-out replacement of Chc1 with Chc1-msGFPx3.

Depositor

Benjamin Glick

Article

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Chc1 portion (CHC1 Budding Yeast)

Use

Tags

msGFPx3

Expression

Yeast

Mutation

Promoter

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pICH50581

Plasmid

#50256

Depositor

Sylvestre Marillonnet

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

promoter, act2 (AT3G18780, Arabidopsis thaliana)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pFF749

Plasmid

#61452

Purpose

IPTG-inducible msd_kanR(ON)_dRT

Depositor

Timothy Lu

Article

Farzadfard et al Science. 2014 Nov 14;346(6211):1256272.

Insert

msd_kanR(ON)_dRT

Use

Synthetic Biology

Tags

Expression

Mutation

D197A D198A

Promoter

pLacO

Available since

Feb. 20, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pLPC FLAG hRap1 delta Br delta M

Plasmid

#13256

Depositor

Titia de Lange

Article

Li et al Mol Biol Cell. 2003 Dec . 14(12):5060-8.

Insert

Repressor Activator Protein 1 delta Br delta M (TERF2IP Human)

Use

Retroviral

Tags

FLAG

Expression

Mutation

contains hRap1 aa2-44 + 184-399 and the point mut…

Promoter

Available since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only
pSL6 - Brr2 del247 WT

Plasmid

#90256

Purpose

protein expression

Depositor

Christine Guthrie

Article

Ledoux et al J Biol Chem. 2016 Jun 3;291(23):11954-65

Insert

BRR2

Use

Tags

TAP

Expression

Yeast

Mutation

deleted first 247 amino acids

Promoter

Available since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pFF761

Plasmid

#61455

Purpose

aTc-inducible SCRIBE_galK(OFF)

Depositor

Timothy Lu

Article

Farzadfard et al Science. 2014 Nov 14;346(6211):1256272.

Insert

SCRIBE_galK(OFF)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

pTetO

Available since

Feb. 20, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pPB-cT3G-cERP2-RAD51

Plasmid

#222567

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of RAD51

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

RAD51 (RAD51 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-lambdaN-HA-DmGW182-delUBA_E

Plasmid

#146256

Purpose

Insect Expression of DmGW182-delUBA

Depositor

Elisa Izaurralde

Article

Eulalio et al RNA. 2009 Apr 21. ():.

Insert

DmGW182-delUBA (gw Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBAD-HisD-iRFP713-HO1

Plasmid

#197256

Purpose

Expresses the protein of iRFP713 and HO1 in bacteria

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

iRFP713

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pTorD-H6

Plasmid

#169039

Purpose

Escherichia coli TorD with C-terminal 6xHis in pET28a.

Depositor

Siegfried Musser

Article

Bageshwar et al PLoS One. 2021 Sep 9;16(9):e0256715. doi

Insert

TorD-H6

Use

Tags

6xHis

Expression

Bacterial

Mutation

The mutation N at position 46 was changed back to…

Promoter

T7

Available since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
A0A090SY56

Plasmid

#163256

Purpose

Inducible expression of UniProt identifier A0A090SY56

Depositor

Martin Engqvist

Article

Rembeza et al PLoS Comput Biol. 2021 Sep 23;17(9):e100

Insert

A0A090SY56

Use

Tags

10xHis and T7

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXBK_aFb

Plasmid

#114256

Purpose

POC12398, MetClo assembly vector with F replication origin and kanamycin resistance for BsaI-based MetClo, adaptor sequence type a-b

Depositor

Christopher A. O'Callaghan

Article

Lin et al Nucleic Acids Res. 2018 Jul 9. pii: 5050

Insert

LacZalpha

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
TAL3082

Plasmid

#41256

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-gbas-Left

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 28, 2012

Availability

Academic Institutions and Nonprofits only
pRAJ31m

Plasmid

#39256

Depositor

Alfonso Jaramillo

Article

Rodrigo et al Proc Natl Acad Sci U S A. 2012 Sep 18;10

Insert

RAJ31 system (no transRNA)

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

pLlacO-1

Available since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
Flag-HA-USP11

Plasmid

#22566

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

USP11 (USP11 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Promoter

Available since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
PB-sgRNA(MS2)

Plasmid

#102560

Purpose

Piggybac transposon sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. Contains BbsI sites for insertion of spacer sequences.

Depositor

Ying Liu

Article

Li et al Mol Ther Nucleic Acids. 2017 Sep 15;8:64

Type

Empty backbone

Use

CRISPR; Piggybac transposon

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 5, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pFsynW TeTx-LC

Plasmid

#197284

Purpose

Encodes for viral expression of the light chain of tetanus toxin.

Depositor

Edwin Chapman

Article

Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755

Insert

Tetanus toxin light chain

Use

Lentiviral

Tags

Expression

Mutation

N/A

Promoter

hSyn (human synapsin I promoter)

Available since

July 28, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge