We narrowed to 29,039 results for: LIS

1. pGLIS3-donor

   Plasmid

   #113812

   Purpose

   CRISPR donor plasmid to tag human transcription factor GLIS3 with GFP

   Depositor

   Kevin White

   Insert

   GLIS3 homology arms flanking EGFP-IRES-Neo cassette (GLIS3 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   rs10118031, rs10973702, rs912257

   Promoter

   Available since

   Dec. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFS_0346_pET30-RT-CRISPR_Bacteroides_fragilis_strain_S14_ARRAY_1_RC

   Plasmid

   #116958

   Purpose

   Expresses BfRT-Cas1-Cas2 under pT7lac promoter, encodes BfCRISPR array 1 RC, compatible with SENECA acquisition readout

   Depositor

   Randall Platt

   Article

   Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

   Insert

   Bacteroides fragilis strain S14 RT-Cas1-Cas2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFS_0345_pET30-RT-CRISPR_Bacteroides_fragilis_strain_S14_ARRAY_1

   Plasmid

   #116957

   Purpose

   Expresses BfRT-Cas1-Cas2 under pT7lac promoter, encodes BfCRISPR array 1, compatible with SENECA acquisition readout

   Depositor

   Randall Platt

   Article

   Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

   Insert

   Bacteroides fragilis strain S14 RT-Cas1-Cas2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAV-U6+27-Tornado-BroccoliSamSensor#1

   Plasmid

   #124363

   Purpose

   Overexpression of circular RNA (Broccoli-based SAM sensor)

   Depositor

   Samie Jaffrey

   Article

   Litke et al Nat Biotechnol. 2019 Apr 8. pii: 10.1038

   Insert

   Tornado-BroccoliSamSensor#1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6+27

   Available since

   June 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. Linked Free ClLIS1 (M)

   Plasmid

   #182487

   Purpose

   Yeast integrative plasmid for expressing fusion protein ERG20(F96W-N127W)-ClLIS1, with linker sequence AG4TGGA (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3)

   Depositor

   Claudia Vickers

   Article

   Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.

   Insert

   FPPS(M)-LS
   

   Use

   Synthetic Biology; Metabolic engineering

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL10

   Available since

   Nov. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFastBac_His-ZZ-TEV-Lis1

   Plasmid

   #132539

   Purpose

   Plasmid for insect cell expression of human full-length LIS1 with an N-terminal His-ZZ-LTLT tag

   Depositor

   Andrew Carter

   Article

   Baumbach et al Elife. 2017 Apr 13;6. pii: e21768. doi:

   Insert

   Lis1 (PAFAH1B1 Human)
   

   Use

   Tags

   8x His, Linker-TEV-linker_TEV, and ZZ

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMXs-GLIS1 (FLJ33315SAAN)

   Plasmid

   #33274

   Purpose

   Retroviral expression of canonical human GLIS1

   Depositor

   Shinya Yamanaka

   Article

   Maekawa et al Nature. 2011 . 474(7350):225-9.

   Insert

   GLIS1, Gateway casette A (GLIS1 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 14, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMT-Flag-RELISH

   Plasmid

   #63750

   Purpose

   Expresses drosophila RELISH tagged with a Flag epitopes, under control of metallothionein promoter

   Depositor

   Cyril Gueydan

   Insert

   Relish (Rel Fly)
   

   Use

   Tags

   Flag

   Expression

   Insect

   Mutation

   Promoter

   pMT

   Available since

   April 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pESC-URA-hPOLISc

   Plasmid

   #60819

   Purpose

   to express human Pol iota recoded to yeast codons in yeast

   Depositor

   Youri Pavlov

   Article

   Makarova et al PLoS One. 2011 Jan 31;6(1):e16612. doi:

   Insert

   POLI (POLI Human)
   

   Use

   Tags

   c-myc

   Expression

   Yeast

   Mutation

   optimized to yeast codon usage

   Promoter

   GAL1

   Available since

   March 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCWXPG-UBI-Glis3M412

    Plasmid

    #114301

    Purpose

    Ubiquitous expression of a constitutively active form of the Glis3 transcription factor

    Depositor

    Patrick Salmon

    Insert

    Glis3 (Glis3 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    N-terminal deletion up to Methionine 412

    Promoter

    Available since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. Coexpressed Free ClLIS1 (M)

    Plasmid

    #182484

    Purpose

    Yeast integrative plasmid for expressing ERG20(F96W-N127W) (GAL10 promoter) and limonene synthase from Citrus limon (ClLIS1; GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

    Depositor

    Claudia Vickers

    Article

    Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.

    Inserts

    FPPS(M)
    LS
    

    Use

    Synthetic Biology; Metabolic engineering

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL10 and GAL7

    Available since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pmiRGLO-GLIS2-201

    Plasmid

    #117331

    Purpose

    Dual luciferase assay for miRNA-targeted UTR

    Depositor

    Volker Busskamp

    Article

    Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

    Insert

    3' UTR GLIS2-201
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Murine PGK-1

    Available since

    Dec. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. ReaLiSM A1-43-GPIba+2cys

    Plasmid

    #59577

    Purpose

    express VWF A1-GPIba ReaLiSM construct for single molecule study

    Depositor

    Timothy Springer

    Insert

    VWF A1-linker-GPIba (VWF Human)
    

    Use

    Tags

    6His

    Expression

    Mammalian

    Mutation

    Promoter

    chick beta-actin

    Available since

    Nov. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

14. CS2-Myc-dLIS1

    Plasmid

    #47047

    Purpose

    Expression of tagged Drosophila LIS1

    Depositor

    Laura Lee

    Article

    Jodoin et al Mol Biol Cell. 2012 Dec;23(24):4713-24.

    Insert

    Lis1 (Lis-1 Fly)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

15. CS2-CHY-dLIS1

    Plasmid

    #47045

    Purpose

    Expression of fluorescent Drosophila LIS1

    Depositor

    Laura Lee

    Article

    Jodoin et al Mol Biol Cell. 2012 Dec;23(24):4713-24.

    Insert

    Lis1 (Lis-1 Fly)
    

    Use

    Tags

    CHY

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pColdII-SIRPT1-Bubalus bubalis

    Plasmid

    #75350

    Purpose

    Nt His-tag Bubalus bubalis Sacsin 86-1364

    Depositor

    Kalle Gehring

    Insert

    Sacsin Bubalus bubalis 86-1364
    

    Use

    Tags

    His-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFastBac_His-ZZ-TEV-Lis1-SNAPf

    Plasmid

    #133073

    Purpose

    Plasmid for insect cell expression of human full-length LIS1 with an N terminal His-ZZ-LTLT tag and C terminal SNAPf tag

    Depositor

    Andrew Carter, Simon Bullock

    Article

    Baumbach et al Elife. 2017 Apr 13;6. pii: e21768. doi:

    Insert

    Lis1 (PAFAH1B1 Human)
    

    Use

    Tags

    8x His, Linker-TEV-linker_TEV, SNAPf, and ZZ

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. PX395 Roseburia intestinalis Cas9

    Plasmid

    #68332

    Purpose

    Roseburia intestinalis Cas9

    Depositor

    Feng Zhang

    Insert

    RiCas9
    

    Use

    CRISPR

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. ReaLiSM A1-Hinge-GPIba+2cys

    Plasmid

    #59581

    Purpose

    express VWF A1-GPIba ReaLiSM construct in mammalian cells

    Depositor

    Timothy Springer

    Article

    Kim et al Nature. 2010 Aug 19;466(7309):992-5. doi

    Insert

    VWF A1-linker-GPIba (VWF Human)
    

    Use

    Tags

    6His

    Expression

    Mammalian

    Mutation

    Promoter

    chick beta-actin

    Available since

    Oct. 31, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pGEX-SIRPT1-Bubalus bubalis

    Plasmid

    #75318

    Purpose

    GST fusion Bubalus bubalis Sacsin 86-1364

    Depositor

    Kalle Gehring

    Insert

    Sacsin Bubalus bubalis 86-1364
    

    Use

    Tags

    GST-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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