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pcDNA3-EngHD

Plasmid

#64033

Purpose

Mammalian expression of TTF-1 homeodomain fused to the N-terminal repressor domain of the drosophila engrailed protein (Eng)

Depositor

Daniel Christophe

Article

Christophe-Hobertus et al Mol Cell Endocrinol. 2007 Jan 29;264(1-2

Insert

TTF-1 (TTF1 dog)

Tags

Eng (N-terminal repressor domain of the drosophi…

Expression

Mammalian

Mutation

homeodomain, aa 154-226

Promoter

CMV

Available Since

April 23, 2015

Availability

Academic Institutions and Nonprofits only
lentiCRISPRv2-TTF-1-gRNA

Plasmid

#101857

Purpose

Introducing a gRNA targeting human TTF-1 (NKX2-1) into cells

Depositor

David Mu

Article

Lai et al Oncogene. 2018 Mar 19. pii: 10.1038/s413

Insert

gRNA targeting TTF-1 (NKX2-1 Human)

Use

Lentiviral

Available Since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pMSCV-puro-TTF-1-HDD

Plasmid

#74991

Purpose

Retroviral vector holding human TTF-1 (NKX2-1) with the homeodomain deleted

Depositor

David Mu

Article

Wood et al Sci Rep. 2016 Feb 25;6:19857. doi: 10.10

Insert

TTF-1 HDD mutant (NKX2-1 Human)

Use

Retroviral

Tags

None

Expression

Mammalian

Mutation

HDD = homeodomain deleted

Available Since

July 27, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA mouse NHERF-1 (1-325)

Plasmid

#32706

Depositor

Edward Weinman

Article

Weinman et al Am J Physiol Renal Physiol. 2001 Aug;281

Insert

solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1 (Nherf1 Mouse)

Tags

none

Expression

Mammalian

Mutation

Deleted last 30 AA putative Ezrin binding site

Promoter

CMV

Available Since

Feb. 28, 2012

Availability

Academic Institutions and Nonprofits only
Puro-TTL-TTF-1-HDD

Plasmid

#74989

Purpose

Human TTF-1 (NKX2-1) cDNA with the homeodomain deleted

Depositor

David Mu

Article

Wood et al Sci Rep. 2016 Feb 25;6:19857. doi: 10.10

Insert

TTF-1 HDD mutant (NKX2-1 Human)

Use

Retroviral

Expression

Mammalian

Mutation

Homeodomain

Available Since

Aug. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pGL3Luc-5XNF-kappaB

Plasmid

#185695

Purpose

Reporter plasmid with a double-strand oligonucleotides that contain five repeats of NF-kappaB binding consensus sequence (5'-GGGGAATTTCC-3').

Depositor

Esther López-Bayghen

Article

Hernandez-Melchor et al ASN Neuro. 2022 Jan-Dec;14:1759091422111

Insert

NF-kappaB (NFKB1 Human)

Use

Luciferase

Promoter

NF-kappaB binding site

Available Since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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MSCV-Nkx2-1/Puro

Plasmid

#31271

Depositor

Tyler Jacks, Monte Winslow

Article

Winslow et al Nature. 2011 May 5. 473(7345):101-4.

Insert

Nkx2-1* (Nkx2-1 Mouse)

Use

Retroviral

Expression

Mammalian

Promoter

MSCV

Available Since

Sept. 27, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-wt-TTF-1

Plasmid

#49989

Purpose

For transient transfection to express human wild type TTF-1 protein

Depositor

David Mu

Article

Runkle et al J Biol Chem. 2012 Aug 17;287(34):28790-8

Insert

TTF-1 (NKX2-1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pMSCV-Hygromycin-TTF-1

Plasmid

#119173

Purpose

Retroviral mammalian expression vector containing wt-human TTF-1 cDNA

Depositor

David Mu

Article

Kendall et al Proc Natl Acad Sci U S A. 2007 Oct 16;10

Insert

NKX2-1 (NKX2-1 Human)

Use

Retroviral

Tags

none

Expression

Mammalian

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-TTF-1-HDD

Plasmid

#52063

Purpose

It encodes a mutant of TTF-1 in which the homeodomain is deleted

Depositor

David Mu

Article

Runkle et al J Biol Chem. 2012 Aug 17;287(34):28790-8

Insert

Thyroid Transcription Factor 1 (NKX2-1 Human)

Expression

Mammalian

Mutation

Homeodomain deleted

Available Since

April 11, 2014

Availability

Academic Institutions and Nonprofits only
pGL2 p15 (-756/+68) SBR2 Mut 3' box

Plasmid

#15711

Depositor

Joan Massague

Article

Gomis et al Cancer Cell. 2006 Sep . 10(3):203-14.

Insert

p15 promoter (CDKN2B Human)

Use

Luciferase

Expression

Mammalian

Mutation

Mut 3' box in SBR2. The 3' box contains…

Available Since

Sept. 7, 2007

Availability

Academic Institutions and Nonprofits only
pMSCV-Zeo-TTF-1

Plasmid

#75089

Purpose

retrovirus-mediated gene transfer into mammalian cells

Depositor

David Mu

Article

Kendall et al Proc Natl Acad Sci U S A. 2007 Oct 16;10

Insert

TTF-1 (NKX2-1 Human)

Use

Retroviral

Tags

none

Expression

Mammalian

Mutation

wild-type, full-length, without native 3' UTR

Available Since

May 31, 2016

Availability

Academic Institutions and Nonprofits only

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pLKO.shNkx2-1

Plasmid

#32400

Depositor

Tyler Jacks, Monte Winslow

Article

Winslow et al Nature. 2011 May 5. 473(7345):101-4.

Insert

shRNA targetting Nkx2-1 (Nkx2-1 Mouse)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Sept. 27, 2011

Availability

Academic Institutions and Nonprofits only
pGL2 p15 4xSBR2 Mut 3' box

Plasmid

#15708

Depositor

Joan Massague

Article

Gomis et al Cancer Cell. 2006 Sep . 10(3):203-14.

Insert

p15 4xSBR2 (CDKN2B Human)

Use

Luciferase

Expression

Mammalian

Mutation

Mutant 3' box. The 3' box contains the …

Available Since

Sept. 7, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(+)-TTF-1-Q50K-Y54M

Plasmid

#75264

Purpose

Expresses a double point mutant of human TTF-1

Depositor

David Mu

Article

Runkle et al J Biol Chem. 2012 Aug 17;287(34):28790-8

Insert

TTF-1 (NKX2-1 Human)

Tags

none

Expression

Mammalian

Mutation

Q50K and Y54M in the homeodomain

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only
pHH0103_SH3D19-4/5

Plasmid

#91426

Purpose

Protein expression and purification of human SH3 domain construct SH3D19-4/5

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3D19-4/5 (SH3D19 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_SH3D19-3/5

Plasmid

#91381

Purpose

Protein expression and purification of human SH3 domain construct SH3D19-3/5

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3D19-3/5 (SH3D19 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_SH3D19-5/5

Plasmid

#91371

Purpose

Protein expression and purification of human SH3 domain construct SH3D19-5/5

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3D19-5/5 (SH3D19 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_SH3D19-1/5

Plasmid

#91339

Purpose

Protein expression and purification of human SH3 domain construct SH3D19-1/5

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3D19-1/5 (SH3D19 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_SH3D19-2/5

Plasmid

#91272

Purpose

Protein expression and purification of human SH3 domain construct SH3D19-2/5

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3D19-2/5 (SH3D19 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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