We narrowed to 1,270 results for: grna cloning vector

1. pLGR002 LGR Cas9 guide vector

   Plasmid

   #188320

   Purpose

   Universal backbone for CRISPR/Cas9 sgRNA expression

   Depositor

   Laralynne Przybyla

   Article

   Heo et al Genome Biol. 2024 Jan 19;25(1):25. doi:

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   PuroR-T2A-mTagBFP2

   Expression

   Mammalian

   Promoter

   pU6 5'CMV and EF1-alpha

   Available Since

   Jan. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pDF0338 pAAV U6-HvsCas7-11 DR Rev-BpiI golden gate backbone dual vector

   Plasmid

   #172514

   Purpose

   Encodes HvsCas7-11 DR and golden gate site for spacer clonings in an AAV backbone with U6 promoter

   Depositor

   Jonathan Gootenberg, Omar Abudayyeh

   Article

   Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

   Insert

   pAAV U6-HvsCas7-11 DR Rev-BpiI golden gate backbone dual vector
   

   Use

   AAV

   Available Since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. STAgR_Neo

   Plasmid

   #102992

   Purpose

   Can be used as backbone for a STAgR reaction

   Depositor

   Stefan Stricker

   Article

   Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

   Type

   Empty backbone

   Use

   CRISPR; Pcr template for stagr vectors

   Promoter

   U6

   Available Since

   Feb. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. STAgR_tdTomato

   Plasmid

   #102993

   Purpose

   Can be used as backbone for a STAgR reaction

   Depositor

   Stefan Stricker

   Article

   Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

   Type

   Empty backbone

   Use

   CRISPR; Pcr template for stagr vectors

   Promoter

   U6

   Available Since

   March 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. STAGR_SAMScaffold_hU6

   Plasmid

   #102843

   Purpose

   Can be used as PCR template for a STAgR reaction

   Depositor

   Stefan Stricker

   Article

   Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

   Insert

   gRNAScaffold_hU6promoter
   

   Use

   As pcr template

   Available Since

   Jan. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. eGFPbait-E2A-KalTA4-pA donor vector

   Plasmid

   #61069

   Purpose

   for CRISPR/Cas9 mediated insertion of E2A-KalTA4; to be used in combination with a eGFP specific sgRNA (e.g. Plasmid 61051)

   Depositor

   Filippo Del Bene

   Article

   Auer et al Genome Res. 2014 Jan;24(1):142-53. doi:

   Inserts

   eGFPbait
   KalTA4
   

   Use

   Zebrafish expression

   Tags

   E2A

   Available Since

   Feb. 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. STAGR_SAMScaffold_mU6

   Plasmid

   #102845

   Purpose

   Can be used as PCR template for a STAgR reaction

   Depositor

   Stefan Stricker

   Article

   Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

   Insert

   STAgR Insert SAM_mU6
   

   Use

   Pcr template for stagr inserts

   Promoter

   mU6

   Available Since

   Nov. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. STAGR_SAMScaffold_hH1

   Plasmid

   #102847

   Purpose

   Can be used as PCR template for a STAgR reaction

   Depositor

   Stefan Stricker

   Article

   Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

   Insert

   STAgR Insert SAM_hH1
   

   Use

   Pcr template for stagr inserts

   Promoter

   U6

   Available Since

   Aug. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. STAGR_SAMScaffold_h7SK

   Plasmid

   #102846

   Purpose

   Can be used as PCR template for a STAgR reaction

   Depositor

   Stefan Stricker

   Article

   Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

   Insert

   STAgR Insert SAM_h7SK
   

   Use

   Pcr template for stagr inserts

   Promoter

   H7SK

   Available Since

   Aug. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDG330

    Plasmid

    #100898

    Purpose

    SpCas9 with a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.

    Depositor

    Paul Thomas

    Article

    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do

    Inserts

    humanized CRISPR associated protein 9 Nickase
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    

    Use

    CRISPR and Mouse Targeting

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CBh and U6

    Available Since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDG459

    Plasmid

    #100901

    Purpose

    SpCas9 with 2A-Puro and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.

    Depositor

    Paul Thomas

    Article

    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do

    Inserts

    humanized CRISPR associated protein 9
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    

    Use

    CRISPR and Mouse Targeting

    Tags

    3xFLAG and T2A-PuroR

    Expression

    Mammalian

    Promoter

    CBh and U6

    Available Since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDG458

    Plasmid

    #100900

    Purpose

    SpCas9 with 2A-EGFP and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.

    Depositor

    Paul Thomas

    Article

    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do

    Inserts

    humanized CRISPR associated protein 9
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    

    Use

    CRISPR and Mouse Targeting

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CBh and U6

    Available Since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDG462

    Plasmid

    #100903

    Purpose

    SpCas9n (D10A Nickase mutant) with 2A-Puro and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of DSBs with no off-target cuts.

    Depositor

    Paul Thomas

    Article

    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do

    Inserts

    humanized CRISPR associated protein 9 Nickase
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    

    Use

    CRISPR and Mouse Targeting

    Tags

    3xFLAG and T2A-PuroR

    Expression

    Mammalian

    Mutation

    D10A mutant converts to Nickase

    Promoter

    CBh and U6

    Available Since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDG461

    Plasmid

    #100902

    Purpose

    SpCas9n (D10A Nickase mutant) with 2A-EGFP and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of DSBs with no off-target cuts.

    Depositor

    Paul Thomas

    Article

    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do

    Inserts

    humanized CRISPR associated protein 9 Nickase
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    

    Use

    CRISPR and Mouse Targeting

    Tags

    3xFLAG and T2A-EGFP

    Expression

    Mammalian

    Mutation

    D10A mutant converts to Nickase

    Promoter

    CBh and U6

    Available Since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDG335

    Plasmid

    #100899

    Purpose

    SpCas9n (D10A Nickase mutant) with a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of DSBs with no off-target cuts.

    Depositor

    Paul Thomas

    Article

    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do

    Inserts

    humanized CRISPR associated protein 9 Nickase
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    

    Use

    CRISPR and Mouse Targeting

    Tags

    HA

    Expression

    Mammalian

    Mutation

    D10A mutant converts to Nickase

    Promoter

    CBh and U6

    Available Since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pYZ173

    Plasmid

    #98410

    Purpose

    Cas9-gRNA lys9 plasmid for lys9 deletion in S. pombe

    Depositor

    Jef Boeke

    Article

    Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

    Insert

    gRNA targeting Sp.lys9 (SPBC3B8.03 Fission Yeast)
    

    Use

    CRISPR

    Available Since

    Feb. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pYZ146

    Plasmid

    #98406

    Purpose

    Cas9-gRNA leu1 plasmid for leu1 deletion in S. pombe

    Depositor

    Jef Boeke

    Article

    Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

    Insert

    gRNA targeting Sp.leu1 (leu1 Fission Yeast)
    

    Use

    CRISPR

    Available Since

    Feb. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pYZ164

    Plasmid

    #98408

    Purpose

    Cas9-gRNA his3 plasmid for his3 deletion in S. pombe

    Depositor

    Jef Boeke

    Article

    Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

    Insert

    gRNA targeting Sp.his3 (his3 Fission Yeast)
    

    Use

    CRISPR

    Available Since

    Feb. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSN007

    Plasmid

    #102440

    Purpose

    For PCR amplification to create paired gRNAs to clone into Golden Gate gRNA expression vectors (e.g. pSB700 Plasmid #64046)

    Depositor

    George Church, Alejandro Chavez

    Article

    Nageshwaran et al J Vis Exp. 2018 Oct 2;(140). doi: 10.379

    Insert

    7SK paired gRNA insert
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Aug. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDual_dsCas9_BFP

    Plasmid

    #214679

    Purpose

    Lentiviral expression vector for an inducible Cas9-P2A-BFP with two empty sgRNA cloning sites

    Depositor

    Kevin Janes

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Available Since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge