We narrowed to 1,130 results for: ncl

1. p5E-mitfa2.1

   Plasmid

   #81234

   Purpose

   zebrafish mitfa promoter entry vector for multisite Gateway cloning

   Depositor

   James Lister

   Insert

   mitfa promoter (mitfa Zebrafish)
   

   Use

   Multisite gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   mitfa, 2.1kb including 5' UTR

   Available since

   Sept. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGR596

   Plasmid

   #213787

   Purpose

   Integrates at the CAN1 locus the BadBoy3 TP-DNAP1 variant and the Nourseothricin resistance marker. Includes an I-SceI transient expression cassette

   Depositor

   Chang Liu

   Insert

   BadBoy2 TP-DNAP1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   See manuscript

   Promoter

   Available since

   March 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGR618

   Plasmid

   #213786

   Purpose

   Integrates at the CAN1 locus the BB-Tv TP-DNAP1 variant and the Nourseothricin resistance marker. Includes an I-SceI transient expression cassette

   Depositor

   Chang Liu

   Insert

   BB-Tv TP-DNAP1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   March 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. p202 Dync1li1 wt

   Plasmid

   #26450

   Depositor

   Elizabeth Fisher

   Insert

   cytoplasmic dynein light intermediate chain 1 (Dync1li1 Mouse)
   

   Use

   Subcloning and sequencing

   Tags

   Expression

   Mutation

   N/A

   Promoter

   Available since

   Nov. 8, 2010

   Availability

   Academic Institutions and Nonprofits only

5. pFB-CT10HF-LIC

   Plasmid

   #39191

   Purpose

   SGC Baculovirus transfer vector with C-terminal His tag and FLAG tag, preceded by a TEV protease cleavage site. Includes sites for LIC cloning, and SacB gene for negative selection

   Depositor

   Nicola Burgess-Brown

   Type

   Empty backbone

   Use

   Baculovirus

   Tags

   TEV-His-FLAG

   Expression

   Mutation

   Promoter

   polyhedrin

   Available since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMSP1D1Q1

   Plasmid

   #97326

   Purpose

   Nanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include an additional Q residue shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.

   Depositor

   Michael Marty

   Insert

   MSP1D1Q1
   

   Use

   Tags

   6xHis - TEV

   Expression

   Bacterial

   Mutation

   Addition of Q residue after T25

   Promoter

   T7

   Available since

   Aug. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. UKC1620-SUP35wt

   Plasmid

   #29730

   Depositor

   Tobias von der Haar

   Insert

   SUP35 gene encoding translation release factor 3 from S. cerevisiae (SUP35 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Wild type gene including genomic sequence 955 bp …

   Promoter

   Available since

   Sept. 6, 2011

   Availability

   Academic Institutions and Nonprofits only

8. pNHA_OROV_NSs

   Plasmid

   #208934

   Purpose

   Expresses N-terminally HA-tagged Oropouche orthobunyavirus NSs protein from CMV promoter in mammalian cells

   Depositor

   Gerald Barry

   Insert

   NSs
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Deleted 69 bp at 5' end (including ATG) and …

   Promoter

   CMV

   Available since

   Jan. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHelper_TS_V2_ampR

   Plasmid

   #214467

   Purpose

   Helper plasmid for ORBIT in E. coli. Expresses RecT for oligo recombineering and Bxb-1 for pInt incorporation. Includes temperature sensitive origin for plasmid curing.

   Depositor

   Scott Saunders

   Article

   Saunders et al bioRxiv 2023

   Insert

   CspRecT / Bxb-1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   XylS / AraC

   Available since

   April 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLVXPuro-mGFP-LIC2

    Plasmid

    #66601

    Purpose

    Expresses Dynein-1 LIC2 as a mGFP fusion

    Depositor

    David Stephens

    Insert

    LIC2 (DYNC1LI2 Human)
    

    Use

    Lentiviral

    Tags

    mGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 28, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pcDNA3.1 CLN3-GFP

    Plasmid

    #78110

    Purpose

    Mammalian expression of human CLN3 with C terminal GFP fusion

    Depositor

    Thomas Braulke

    Insert

    CLN3 (CLN3 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 20, 2016

    Availability

    Academic Institutions and Nonprofits only

12. pAAV-CAG-RLuc

    Plasmid

    #83282

    Purpose

    recombinant AAV vector packaging single-stranded Renilla Luciferase under the CAG promoter (includes WPRE element)

    Depositor

    Mark Kay

    Insert

    Renilla Luciferase
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLVXPuro-mGFP-LIC1

    Plasmid

    #66600

    Purpose

    Expresses Dynein-1 LIC1 as a mGFP fusion

    Depositor

    David Stephens

    Insert

    LIC1 (DYNC1LI1 Human)
    

    Use

    Lentiviral

    Tags

    mGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 24, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pSC301

    Plasmid

    #31851

    Depositor

    Yossef Av-Gay

    Article

    Cowley et al Gene. 2001 Feb 21;264(2):225-31.

    Insert

    GFP (including ATG start codon)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    SOD

    Available since

    Nov. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

15. 9 pCOLI_A_C_GST

    Plasmid

    #160245

    Purpose

    C-term GST uncleavable Amp resistant bacterial expression vector

    Depositor

    John Weir

    Article

    Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

    Type

    Empty backbone

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTH671-SUP35-H348Q

    Plasmid

    #29732

    Depositor

    Tobias von der Haar

    Insert

    SUP35 gene encoding translation release factor 3 from S. cerevisiae (SUP35 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    H348Q mutant gene of SUP35 including genomic sequ…

    Promoter

    Available since

    Sept. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. 57 pLIB_C_CBP

    Plasmid

    #160306

    Purpose

    C-term uncleavable CBP tag for insect cell expression

    Depositor

    John Weir

    Article

    Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    polyhydrin

    Available since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. 45 pLIB_C_12HIS

    Plasmid

    #160271

    Purpose

    C-term uncleavable 12xHis tag for insect cell expression

    Depositor

    John Weir

    Article

    Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

    Type

    Empty backbone

    Use

    Tags

    12xHis

    Expression

    Insect

    Mutation

    Promoter

    polyhydrin

    Available since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28-Flag-RBM24-1~93

    Plasmid

    #208633

    Purpose

    for E. coli expression of truncated Flag tagged human RBM24 (ENST00000379052), aa1~93

    Depositor

    Quanfu Ma

    Insert

    human RBM24 (ENST00000216146) aa 1~93 (RBM24 Human)
    

    Use

    Tags

    6xHis and Flag

    Expression

    Bacterial

    Mutation

    only aa 1~93, other residues not included

    Promoter

    T7

    Available since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. 38 pCOLI_K_ C_STREP

    Plasmid

    #160266

    Purpose

    C-term 2xStrepII uncleavable Kan resistant bacterial expression vector

    Depositor

    John Weir

    Article

    Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

    Type

    Empty backbone

    Use

    Tags

    2xStrepII

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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