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pCS-Fv2Eflt4um200-2a-tdTomato

Plasmid

#67698

Purpose

Expression construct

Depositor

Nathan Lawson

Article

Shin et al Development. 2016 Oct 15;143(20):3785-37

Insert

Fv2E-flt4-2A-tdTomato (flt4 Zebrafish)

Tags

2A-tdTomato and HA

Mutation

um200

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only
LentiV_Neo_SIK3_CR

Plasmid

#108104

Purpose

Lentiviral expression plasmid of human SIK3 cDNA (CRISPR-resistant silent mutation) with neomycin resistance gene

Depositor

Christopher Vakoc

Article

Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

Insert

SIK3 (SIK3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

change guanine 501 to cytosine (silent mutation)

Promoter

EFS promoter

Available Since

April 5, 2018

Availability

Academic Institutions and Nonprofits only

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pBA439

Plasmid

#85967

Purpose

Perturb-seq vector backbone

Depositor

Jonathan Weissman

Article

Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

Inserts

sgGFP-NT2
puro-T2A-BFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pDY1330 STITCHR pCMV-nCas9-XTEN-v170_R2Tocc

Plasmid

#234826

Purpose

STITCHR R2Tocc editor

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Fell et al Nature. 2025 Apr 9. doi: 10.1038/s41586-

Insert

nCas9h840a-xten-R2Tocc

Use

CRISPR

Available Since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA4-NHK-MYC-His6

Plasmid

#185350

Purpose

Assessing of ubiquitination in cells

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

a1-antitrypsin Hong Kong Null mutant

Tags

Myc, 6xHis

Expression

Mammalian

Mutation

NHK mutant

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pAAVS1-PC-GCaMP6f

Plasmid

#73503

Purpose

GCaMP6f knock in construct into the AAVS1 locus

Depositor

Bruce Conklin

Article

Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

Insert

GCaMP6f

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pLV-Hygro-EF1A-KR-Linker-eGFP

Plasmid

#176066

Purpose

EGFP fused to the C-terminus of a KR domain & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

Insert

KR

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1A

Available Since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pSpCTRE-CD4

Plasmid

#114010

Purpose

Dox-inducible Cas9 expression with a CD4 selection marker

Depositor

Charles Rudin

Article

Hulton et al Nat Cancer. 2020 Mar;1(3):359-369. doi:

Insert

SpCas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

March 10, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA4-RI1-332-MYC-His6

Plasmid

#185351

Purpose

Mammalian expression of Ribophorin I

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

Ribophorin I (RPN1 Human)

Tags

Myc, 6xHis

Expression

Mammalian

Mutation

aa 1-332 only

Available Since

Feb. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pECE-M2-PP1Cbeta

Plasmid

#31677

Depositor

Anne Brunet

Article

Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

Insert

protein phosphatase 1, catalytic subunit, beta isozyme (PPP1CB Human)

Tags

M2

Expression

Mammalian

Available Since

April 11, 2012

Availability

Academic Institutions and Nonprofits only
PCMV-intron myc Rab1a S25N

Plasmid

#46777

Purpose

expression of S25N Rab1a in mammalian cells

Depositor

Terry Hébert

Article

Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

Insert

Rab1a S25N mutant (RAB1A Human)

Tags

myc

Expression

Mammalian

Mutation

S25N (dominant negative)

Promoter

CMV

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only
pFLAG AXL

Plasmid

#105933

Purpose

Mammalian expression of Flag-tagged AXL

Depositor

Rosa Marina Melillo

Article

Krishnamoorthy et al J Biol Chem. 2013 Jun 14;288(24):17481-9

Insert

AXL (AXL Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2018

Availability

Academic Institutions and Nonprofits only

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h-Plekhm1_EGFP

Plasmid

#73836

Purpose

Express human Plekhm1 in mammalian cells

Depositor

Paul Odgren

Article

Van Wesenbeeck et al J Clin Invest. 2007 Apr;117(4):919-30.

Insert

Plekhm1 (PLEKHM1 Human)

Tags

GFP

Expression

Mammalian

Available Since

March 15, 2016

Availability

Academic Institutions and Nonprofits only

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pBABEpuro-ARAF

Plasmid

#51076

Purpose

mammalian expression of wt ARAF

Depositor

Matthew Meyerson

Article

Imielinski et al J Clin Invest. 2014 Apr;124(4):1582-6. d

Insert

ARAF (ARAF Human)

Use

Retroviral

Expression

Mammalian

Available Since

Feb. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pIRES-Puro-MGMT

Plasmid

#187654

Purpose

Expresses hMGMT together with the puromycin resistance gene via an IRES.

Depositor

Robert Sobol

Article

Li et al Cancers (Basel). 2022 Jul 22;14(15). pii

Insert

MGMT (MGMT Human)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

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sgTrack-BFP

Plasmid

#164273

Purpose

Empty sgRNA expression vector with co-expression of BFP reporter

Depositor

Charles Rudin

Article

Wohlhieter et al Cell Rep. 2020 Dec 1;33(9):108444. doi:

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 and EFS

Available Since

Feb. 3, 2021

Availability

Academic Institutions and Nonprofits only

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AAV-p3-PE(1153-dRnH)-C-term

Plasmid

#187182

Purpose

p3-PE2-C-term(1153-dRnH) in AAV2 backbone (ITRs) targeting DNMT1

Depositor

Gerald Schwank

Article

Bock et al Sci Transl Med. 2022 Mar 16;14(636):eabl

Insert

PE2 from plasmid #132775 with shortened RnaseH domain

Use

AAV

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pECE-HA-AMPKbeta1 wt

Plasmid

#31666

Depositor

Anne Brunet

Article

Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

Insert

protein kinase, AMP-activated, beta 1 non-catalytic subunit (PRKAB1 Human)

Tags

HA (YDVPDYASL)

Expression

Mammalian

Available Since

April 11, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-CUL1 K720A

Plasmid

#19939

Depositor

Yue Xiong

Article

Furukawa et al Mol Cell Biol. 2000 Nov . 20(21):8185-97

Insert

cullin 1 K720A (CUL1 Human)

Tags

HA2

Expression

Mammalian

Mutation

CUL1 with a NEDD8 modification mutant

Available Since

April 17, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA3-HA-CUL1 Y42A/M43A

Plasmid

#19940

Depositor

Yue Xiong

Article

Furukawa et al Mol Cell Biol. 2000 Nov . 20(21):8185-97

Insert

cullin 1 Y42A/M43A (CUL1 Human)

Tags

HA2

Expression

Mammalian

Mutation

CUL1 with a SKP1 binding mutant

Available Since

May 5, 2009

Availability

Academic Institutions and Nonprofits only