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pcDNA3_attR1-ORF-attR2-NTEV-TCS-GV-2xHA_DEST

Plasmid

#194385

Purpose

Gateway Destination vector for split TEV assays

Depositor

Michael Wehr

Article

Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

Insert

attR1-ORF-attR2-NTEV-TCS-GV-2xHA

Tags

2xHA

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti CMV Puro DEST (w118-1) MXRA7

Plasmid

#107500

Purpose

Expresses MXRA7, puromycin resistant

Depositor

Alejandro Gutierrez

Article

Aries et al J Exp Med. 2018 Nov 7. pii: jem.20180570

Insert

MXRA7 (codon optimized) (MXRA7 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Codon optimized sequence

Promoter

CMV

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pDN0605 (pDEST-DHFR F[1,2] N-term, NatR)

Plasmid

#210487

Purpose

pDEST vector to tag gene of interest with DHFR F[1,2] on the N-terminus to perform DHFR-PCA in S. cerevisiae.

Depositor

Christian Landry

Article

Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

Type

Empty backbone

Use

Synthetic Biology; Destination vector for gateway…

Expression

Yeast

Promoter

ADH1

Available Since

Dec. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti CMV Puro DEST (w118-1) VOPP1

Plasmid

#107507

Purpose

expressing VOPP1, puromycin resistant

Depositor

Alejandro Gutierrez

Article

Aries et al J Exp Med. 2018 Nov 7. pii: jem.20180570

Insert

VOPP1 (VOPP1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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Tol2-LyzC-GFP-U6ac-rac2-guides

Plasmid

#168241

Purpose

"neutrophil specific GFP with ubiquitous rac2 sgRNAs"

Depositor

Qing Deng

Article

Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

Insert

rac2 sgRNAs

Use

CRISPR

Available Since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST40-2XHA-Src-K298M-Y419F-Y530F

Plasmid

#140320

Purpose

Expresses HA-tagged human Src-K298M-Y419F-Y530F in mammalian cells for structure-function studies

Depositor

Mark Moasser

Article

Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

Insert

Src mutant (SRC Human)

Tags

2X HA

Expression

Mammalian

Mutation

K298M-Y419F-Y530F

Promoter

CMV

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCSDest2-SpCas9-NLS-3XHA-NLS-Zif268

Plasmid

#69219

Purpose

Expresses wild type SpCas9 fused to Zif268 in mammalian cells

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

Insert

Zif268 (Egr1 Mouse)

Use

CRISPR

Tags

NLS-3XHA-NLS

Expression

Mammalian

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pLenti CMV Puro DEST (w118-1) FAM149A

Plasmid

#107510

Purpose

Expressing FAM149a, puromycin resistant

Depositor

Alejandro Gutierrez

Article

Aries et al J Exp Med. 2018 Nov 7. pii: jem.20180570

Insert

FAM149A (FAM149A Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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Tol2-LyzC-GFP-U6ac-cdk2-guides

Plasmid

#168250

Purpose

"neutrophil specific GFP with ubiquitous cdk2 sgRNAs"

Depositor

Qing Deng

Article

Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

Insert

cdk2 sgRNAs

Use

CRISPR

Available Since

March 16, 2022

Availability

Academic Institutions and Nonprofits only

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pCSDest2-SpCas9-WT-NLS-3XHA-NLS-ZFP_TS4

Plasmid

#69227

Purpose

Expresses wild type SpCas9 fused to ZFP-TS4 in mammalian cells

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

Insert

TS4 ZFP

Use

CRISPR

Tags

NLS-3XHA-NLS

Expression

Mammalian

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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