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lenti-fullef1a-FAKSPARK2

Plasmid

#195265

Purpose

express wtFAKSPARK2 in a lenti vector

Depositor

Xiaokun Shu

Article

Li et al Nat Chem Biol. 2023 Jun 22. doi: 10.1038

Insert

FAKSPARK2 sensor

Use

Lentiviral

Expression

Mammalian

Promoter

ef1a

Available Since

Nov. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pQCXI Neo DsRed-LC3-GFP

Plasmid

#31183

Depositor

David Sabatini

Article

Sheen et al Cancer Cell. 2011 May 17. 19(5):613-28.

Insert

DsRed-LC3-GFP

Use

Retroviral

Expression

Mammalian

Available Since

June 17, 2011

Availability

Academic Institutions and Nonprofits only

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pTol2-elavl3_NES-rsCaMPARI-mRuby3

Plasmid

#122129

Purpose

Pan-neuronal expression of rsCaMPARI in zebrafish, nucleus excluded

Depositor

Eric Schreiter

Article

Sha et al Elife. 2020 Sep 15;9. pii: 57249. doi: 1

Insert

rsCaMPARI

Use

Zebrafish expression

Tags

NES-His and mRuby3

Promoter

elavl3

Available Since

Oct. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pDestTol2-QUAS:GCaMP7a

Plasmid

#184817

Purpose

Used to generate the Tg(QUAS:GCaMP7a)c594 transgenic line. GCaMP7a from Muto et al., 2013 (https://doi.org/10.1016/j.cub.2012.12.040).

Depositor

Marnie Halpern

Article

Spikol et al Elife. 2024 May 31;12:RP89516. doi: 10.7

Insert

QUAS:GCaMP7a-pA

Use

Zebrafish tol2 transgenesis

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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mCherry-ITSN2

Plasmid

#129615

Purpose

RhoGEF

Depositor

Dorus Gadella

Article

Reinhard et al Small GTPases. 2019 Aug 30:1-15. doi: 10

Insert

ITSN2

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pRL542

Plasmid

#70282

Purpose

ColK-based vector; provides a nicking function to permit conjugal transfer and bears a polylinker for insertion of methylase-encoding genes

Depositor

Peter Wolk

Article

Elhai et al J Bacteriol. 1997 Mar;179(6):1998-2005.

Type

Empty backbone

Expression

Bacterial

Available Since

Feb. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pGEX-XIAP

Plasmid

#8340

Depositor

Jon Ashwell

Article

Yang et al Science 2000 May 5;288(5467):874-7.

Insert

XIAP (XIAP Human)

Tags

GST

Expression

Bacterial

Available Since

March 14, 2006

Availability

Academic Institutions and Nonprofits only

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pKL11-GCaMP6f bb100

Plasmid

#158983

Purpose

Constitutive expression (sigma 70 promoter) of GCaMP6f fluorophore in E. coli.

Depositor

Joel Kralj

Article

Bruni et al eLife. 2020 Aug 4;9. pii: 58706. doi: 10

Insert

GCaMP6f

Expression

Bacterial

Mutation

codon optimized for e. coli from Addgene#100833

Promoter

Bba_J23100 (sigma 70)

Available Since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-Cre-MS2_UbC-GFP (Cre_GFP)

Plasmid

#219536

Purpose

a. Expresses Cre recombinase tagged with 21x repeats of MS2 binding sequence under EF1A promotor and b. GFP reporter under UbC promotor

Depositor

Jeffrey Gerst

Article

Haimovich et al bioRxiv 2024.11.06.622258

Inserts

Cre recombinase (cre )
GFP

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 11, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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LRT2B-GO2

Plasmid

#136909

Purpose

Lentivirus expressing GO2 sgRNA

Depositor

Lukas Dow

Article

Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

Insert

sgGO2

Use

Lentiviral

Mutation

WT

Promoter

hU6

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pJV0047

Plasmid

#205139

Purpose

Expression of 6xHis-EGFP-2xPX in bacteria

Depositor

Jason King

Article

Vines et al J Cell Biol. 2023 Sep 4;222(9):e20220907

Insert

tandem 2xPX domain of SnxA

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 4, 2023

Availability

Academic Institutions and Nonprofits only

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PiggyBac-G13-sensor

Plasmid

#137809

Purpose

A FRET sensor for G13 activation, encoded on a single plasmid

Depositor

Dorus Gadella

Article

Bordes et al Methods Mol Biol. 2021;2268:159-178. doi

Insert

Galpha13 sensor

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-BRIC

Plasmid

#172341

Purpose

AAV transfer plasmid for BRIC (Bioluminescent Red Indicator for Calcium)

Depositor

Huiwang Ai

Article

Tian et al Nat Commun. 2022 Jul 8;13(1):3967. doi:

Insert

BRIC

Use

AAV

Promoter

hSyn promoter

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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paavCAG-pre-mGRASP-2A-dTomato

Plasmid

#51902

Purpose

pre-2Ato

Depositor

Jinhyun Kim

Article

Kim et al Nat Methods. 2011 Dec 4;9(1):96-102. doi

Insert

presynaptic mGRASP

Use

AAV and Cre/Lox

Available Since

Jan. 27, 2015

Availability

Academic Institutions and Nonprofits only

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mCherry-FGD1

Plasmid

#129612

Purpose

RhoGEF

Depositor

Dorus Gadella

Article

Reinhard et al Small GTPases. 2019 Aug 30:1-15. doi: 10

Insert

FGD1

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pRL-ubi63E

Plasmid

#74280

Purpose

Renilla control plasmid driven by the ubiquitin 63E promoter

Depositor

Alexander Stark

Article

Arnold et al Science. 2013 Mar 1;339(6123):1074-7. do

Insert

Renilla luciferase

Expression

Insect

Promoter

ubiquitin 63E promoter

Available Since

April 6, 2016

Availability

Academic Institutions and Nonprofits only

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PAS528 (TJF059)

Plasmid

#65602

Purpose

pETDuet-1 Containing FadD-His

Depositor

Pamela Silver

Article

Ford et al PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717

Insert

FadD

Tags

6x His

Expression

Bacterial

Promoter

T7 lacO

Available Since

June 2, 2015

Availability

Academic Institutions and Nonprofits only
UAS-myr::4xSNAPf

Plasmid

#87637

Purpose

For creating drosophila transgenics expressing UAS-myr4x::SNAPf

Depositor

Gregory Jefferis

Article

Sutcliffe et al Genetics. 2017 Apr;205(4):1399-1408. doi

Insert

myr::4xSNAPf

Expression

Insect

Available Since

March 27, 2017

Availability

Academic Institutions and Nonprofits only

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pRibo EcoHind

Plasmid

#36252

Depositor

Carolyn Bertozzi, Jessica Seeliger

Article

Seeliger et al PLoS One. 2012;7(1):e29266. Epub 2012 Ja

Type

Empty backbone

Use

Synthetic Biology; Mycobacteria replicating vector

Expression

Bacterial

Promoter

Modified Hsp60

Available Since

April 9, 2012

Availability

Academic Institutions and Nonprofits only

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pET28a-T4uvsW

Plasmid

#228201

Purpose

Expression in BL21(DE3) of T4 3' to 5' helicase/single-strand DNA annealase

Depositor

Stephen Benkovic

Article

Nelson et al J Biol Chem. 2007 Jan 5;282(1):407-16. d

Insert

uvsW

Tags

hexahistidine and 10 amino acid linker

Expression

Bacterial

Promoter

T7

Available Since

March 6, 2025

Availability

Academic Institutions and Nonprofits only

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