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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 6,471 results for: poly

Showing: 4381 - 4400 of 6471 results

  1. pTSara-R756S

    Plasmid
    #60725
    Purpose
    Expresses both fragments of T7 RNAP split at position 179. Both fragments are driven by PBAD. The C-terminal fragment has the point mutation R756S. Contains a constitutive araC ORF
    Depositor
    Matthew Bennett
    Article
    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11
    Inserts
    Residues 1-179 of split T7 RNAP
    Residues 180-880 of split T7 RNAP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Residues 1-180 of split T7 RNAP and Residues 180-…
    Promoter
    Available since
    Dec. 18, 2014
    Availability
    Academic Institutions and Nonprofits only
    60725-plasmid-map-sequence-id-97713

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  2. pTSara-Q758C

    Plasmid
    #60724
    Purpose
    Expresses both fragments of T7 RNAP split at position 179. Both fragments are driven by PBAD. The C-terminal fragment has the point mutation Q758C. Contains a constitutive araC ORF
    Depositor
    Matthew Bennett
    Article
    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11
    Inserts
    Residues 1-179 of split T7 RNAP
    Residues 180-880 of split T7 RNAP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Residues 1-180 of split T7 RNAP and Residues 180-…
    Promoter
    Available since
    Dec. 18, 2014
    Availability
    Academic Institutions and Nonprofits only
    60724-plasmid-map-sequence-id-97710

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  9. pCrysβ:ECFP-LexOP:mCherry-KrasG12V

    Plasmid
    #89888
    Purpose
    Human KRasG12V fused to mCherry downstream of LexOP, placed within a non-autonomous maize dissociation (Ds) element for integration in zebrafish. Contains pCrysB-ECFP for screening.
    Depositor
    Tatjana Sauka-Spengler
    Article
    Kenyon et al Dis Model Mech. 2018 Apr 6;11(4). pii: d
    Insert
    mCherry-KRasG12V
    Use
    Unspecified
    Tags
    mCherry
    Expression
    Mutation
    Promoter
    Available since
    Aug. 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    89888-plasmid-map-sequence-id-181093

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  13. pRMC2-tat:msfGFP

    Plasmid
    #194915
    Purpose
    Tetracycline inducible expression of msfGFP fused to Tat signal peptide in Staphylococci
    Depositor
    Rikke Meyer
    Article
    Evans et al Microb Cell. 2023 Jun 28;10(7):145-156.
    Insert
    Shine Dalgarno sequence followed by Tat signal peptide fused to monomeric superfolder GFP
    Use
    Tetracycline-inducible expression vector for stap…
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    194915-plasmid-map-sequence-id-384626

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  14. pcDNA-dCas9-HA

    Plasmid
    #61355
    Purpose
    encodes c-terminal HA-tagged S. pyogenes dCas9 driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal HA tag
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    CMV
    Available since
    April 8, 2015
    Availability
    Academic Institutions and Nonprofits only
    61355-plasmid-map-sequence-id-99781

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  15. pRMC2-sec:msfGFP

    Plasmid
    #194914
    Purpose
    Tetracycline inducible expression of msfGFP fused to Sec signal peptide in Staphylococci
    Depositor
    Rikke Meyer
    Article
    Evans et al Microb Cell. 2023 Jun 28;10(7):145-156.
    Insert
    Shine Dalgarno sequence followed by Sec signal peptide fused to monomeric superfolder GFP
    Use
    Tetracycline-inducible expression vector for stap…
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    194914-plasmid-map-sequence-id-384625

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  16. pCrysβ:ECFP-LexOP:mCherry-NRasQ61K

    Plasmid
    #89886
    Purpose
    Human NRasQ61K fused to mCherry downstream of LexOP, placed within a non-autonomous maize dissociation (Ds) element for integration in zebrafish. Contains pCrysB-ECFP for screening.
    Depositor
    Tatjana Sauka-Spengler
    Article
    Kenyon et al Dis Model Mech. 2018 Apr 6;11(4). pii: d
    Insert
    mCherry-NRasQ61K
    Use
    Unspecified
    Tags
    mCherry
    Expression
    Mutation
    Promoter
    Available since
    Aug. 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    89886-plasmid-map-sequence-id-180692

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  17. pCrysβ:ECFP-LexOP:mCherry-HrasG12V

    Plasmid
    #89887
    Purpose
    Human HRasG12V fused to mCherry downstream of LexOP, placed within a non-autonomous maize dissociation (Ds) element for integration in zebrafish. Contains pCrysB-ECFP for screening.
    Depositor
    Tatjana Sauka-Spengler
    Article
    Kenyon et al Dis Model Mech. 2018 Apr 6;11(4). pii: d
    Insert
    mCherry-HRasG12V
    Use
    Unspecified
    Tags
    mCherry
    Expression
    Mutation
    Promoter
    Available since
    Aug. 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    89887-plasmid-map-sequence-id-181092

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  18. PVRL2-bio-His

    Plasmid
    #51824
    Purpose
    Expresses full-length Poliovirus receptor-related protein 2 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    PVRL2 (NECTIN2 Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    51824-plasmid-map-sequence-id-79661

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Showing: 4381 - 4400 of 6471 results