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Showing: 10951 - 11000 of 23409 results

  1. pCas9_sgRNA_0

    Plasmid
    #70763
    Purpose
    expresses Ustilago maydis codon-optimized Cas9, contains U. maydis U6 promoter, is self-replicating
    Depositor
    Regine Kahmann
    Article
    Schuster et al Fungal Genet Biol. 2015 Sep 11. pii: S10
    Inserts
    U6 promoter
    cas9
    tnos terminator
    Use
    Self-replicating in ustilago maydis, conferring c…
    Tags
    N-terminal NLS, C-terminal HA-tag +NLS
    Expression
    Mutation
    Streptococcus pyogenes gene codon-optimized for U…
    Promoter
    U6 from Ustilago maydis and synthetic otef promot…
    Available since
    Nov. 12, 2015
    Availability
    Academic Institutions and Nonprofits only
    70763-plasmid-map-sequence-id-240922

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  7. ATP1A1_plasmid_donor_RD

    Plasmid
    #86551
    Purpose
    Homology-directed repair (HDR) donor plasmid with homology arms to ATP1A1 to introduce the Q118R and N129D (RD) mutations conferring cellular resistance to ouabain
    Depositor
    Yannick Doyon
    Article
    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n
    Insert
    ATP1A1 (ATP1A1 Human)
    Use
    CRISPR and TALEN; Co-selection via hdr using ouab…
    Tags
    Expression
    Mammalian
    Mutation
    Q118R, N129D
    Promoter
    Available since
    March 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    86551-plasmid-map-sequence-id-171421

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  8. qTAG-N-Puro-mStayGold-ACTB

    Plasmid
    #227320
    Purpose
    Donor template for Puro-2A-mStayGold insertion into the N-terminus of the ACTB locus. For actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB (Addgene #207748)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    ACTB Homology Arms flanking a Puro-2A-mStayGold Cassette (ACTB Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Promoterless
    Available since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227320-plasmid-map-sequence-id-452091

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  10. qTAG-C-Solo-mStayGold-CLTC

    Plasmid
    #227313
    Purpose
    Donor template for mStayGold insertion into the C-terminus of the CLTC locus. For clathrin heavy chain visualization. To be co-transfected with sgRNA px330-PITCh-CLTC (Addgene #227312)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    CLTC Homology Arms flanking a mStayGold Tag (CLTC Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Promoterless
    Available since
    Nov. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    227313-plasmid-map-sequence-id-451694

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  14. pLM-AMA15.0 Cas9

    Plasmid
    #138944
    Purpose
    Fungal AMA1 plasmid with sp-Cas9, ribozymes based "plug-and-play" sgRNA transcription unit, Terbinafine and Phleomycin selection markers
    Depositor
    Arnold Driessen
    Article
    Mozsik et al Sci Rep. 2021 Jan 13;11(1):1118. doi: 10
    Insert
    p40S:sp_Cas9_2xNLS; HH-HDV sgRNA transcription unit, Terbinafine (ergA) and Phleomycin (ble) selection markers
    Use
    CRISPR and Synthetic Biology; Ama1 autonomously r…
    Tags
    NLS
    Expression
    Mutation
    Promoter
    p40S (AN0465) Aspergillus nidulans
    Available since
    Jan. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    138944-plasmid-map-sequence-id-364383

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  16. qTAG-N-Puro-moxGFP-LMNB1

    Plasmid
    #207773
    Purpose
    Donor template for Puro-2A-moxGFP insertion into the N-terminus of the LMNB1 locus for nuclear envelope visualization. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    LMNB1 Homology Arms flanking a Puro-moxGFP Cassette (LMNB1 Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 15, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207773-plasmid-map-sequence-id-413797

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  19. qTAG-N-Puro-moxGFP-GOLGA2

    Plasmid
    #207792
    Purpose
    Donor template for Puro-2A-moxGFP insertion into the N-terminus of the GOLGA2 locus for Golgi visualization. To be co-transfected with sgRNA plasmid px330-PITCh-GOLGA2 Addgene #207791
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    GOLGA2 Homology Arms flanking a Puro-moxGFP Cassette (GOLGA2 Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 15, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207792-plasmid-map-sequence-id-413795

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  23. qTAG-N-Blast-dTAG-LMNB1

    Plasmid
    #207776
    Purpose
    Donor template for Blast-2A-dTAG insertion into the N-terminus of the LMNB1 locus. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    LMNB1 Homology Arms flanking a Blast-dTAG Cassette (LMNB1 Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 29, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207776-plasmid-map-sequence-id-416195

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  24. pSLPB2B-FKBP12_F36V-SpdCas9-hHDAC4-tagBFP-PGK-Blasticidin

    Plasmid
    #187954
    Purpose
    FKBP12 (F36V mutant) degron-tagged dCas9-hHDAC4 fused to tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.
    Depositor
    Edda Schulz
    Article
    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:
    Insert
    FKBP12_F36V-SpdCas9-hHDAC4-tagBFP
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    187954-plasmid-map-sequence-id-373498

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  27. pETM6-4F2-mCherry

    Plasmid
    #73421
    Purpose
    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 4F2.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Promoter 4F2 (orthogonal T7-lac variant)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    P4F2 (orthogonal T7-lac variant)
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    73421-plasmid-map-sequence-id-135321

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  28. qTAG-N-Blast-ultraID-LMNB1

    Plasmid
    #207775
    Purpose
    Donor template for Blast-2A-ultraID insertion into the N-terminus of the LMNB1 locus. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    LMNB1 Homology Arms flanking a Blast-ultraID Cassette (LMNB1 Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 29, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207775-plasmid-map-sequence-id-416196

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  30. pJSC281 - Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), REC2 FRET variant

    Plasmid
    #101230
    Purpose
    Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), REC2 FRET variant
    Depositor
    Keith Joung, Jennifer Doudna
    Article
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    SpCas9 variant C80S/C574S/E60C/D273C/N692A/M694A/Q695A/H698A
    Use
    Tags
    10x His, MBP, and TEV site
    Expression
    Bacterial
    Mutation
    C80S, C574S, E60C, D273C, N692A, M694A, Q695A and…
    Promoter
    T7
    Available since
    Nov. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    101230-plasmid-map-sequence-id-198024

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  31. pNon-target

    Plasmid
    #214053
    Purpose
    Expresses non-target sequence for Haliangium type III CRISPR-Cas complex; identical to pTarget (Addgene plasmid # 214052) but does not contain a protospacer.
    Depositor
    Raymond Staals
    Article
    Steens et al Science. 2024 Feb 2;383(6682):512-519. d
    Insert
    Non-target sequence
    Use
    Non-target rna plasmid
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    trc promoter
    Available since
    June 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    214053-plasmid-map-sequence-id-428839

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  32. Pblu-AAVS1-Cas9-p300-M2rtTA-AAVS1

    Plasmid
    #112261
    Purpose
    Donor plasmid with a Cas9-p300 expression cassette, a M2rtTA expression cassette, a T2A-puro selection cassette, and two gRNA recognition sequences at both ends of the whole insertion DNA fragment.
    Depositor
    Zhili Rong
    Article
    Ma et al FEBS Lett. 2018 Jun 5. doi: 10.1002/1873
    Insert
    spCas9-p300 (EP300 Synthetic, Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    112261-plasmid-map-sequence-id-222598

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  37. pJEP308-pAAV-EFS-dSaCas9-VP64-Dio-pA

    Plasmid
    #113685
    Purpose
    An EFS driven inverted de-catalyzed SaCas9 fused to the transcription activator VP64. dSaCas9-VP64 is floxed to render the system cre-dependent.
    Depositor
    Jonathan Ploski
    Article
    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.
    Insert
    inverted de-catalyzed SaCas9
    Use
    AAV, CRISPR, Cre/Lox, and Synthetic Biology
    Tags
    NLS and VP64
    Expression
    Mutation
    Promoter
    Available since
    Dec. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    113685-plasmid-map-sequence-id-232027

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  38. qTAG-C-moxGFP-Puro-MAPRE1

    Plasmid
    #207794
    Purpose
    Donor template to insert moxGFP-2A-Puro into the C-terminus of the MAPRE1 locus for growing microtubule tip visualization. To be co-transfected with sgRNA plasmid px330-PITCh-MAPRE1 Addgene #207793
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    MAPRE1 Homology Arms flanking a moxGFP-Puro Cassette (MAPRE1 Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 1, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207794-plasmid-map-sequence-id-416380

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  41. qTAG-N-Puro-mScarlet-ACTB

    Plasmid
    #207754
    Purpose
    Donor template for Puro-2A-mScarlet insertion into the N-terminus of the ACTB locus for actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB Addgene #207748
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    ACTB Homology Arms flanking a Puro-mScarlet Cassette (ACTB Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 16, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207754-plasmid-map-sequence-id-415028

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  43. pCC_03 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-xCas9-NLS-2A-Puro-WPRE

    Plasmid
    #139088
    Purpose
    Expresses human codon-optimized xCas9 3.7 nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Article
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    xCas9 3.7
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    A262T, R324L,S409I, E480K, E543D, M694I, E1219V
    Promoter
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139088-plasmid-map-sequence-id-273884

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  47. qTAG-N-Blast-mScarlet-ACTB

    Plasmid
    #207753
    Purpose
    Donor template for Blast-2A-mScarlet insertion into the N-terminus of the ACTB locus for actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB Addgene #207748
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    ACTB Homology Arms flanking a Blast-mScarlet Cassette (ACTB Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 1, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207753-plasmid-map-sequence-id-416363

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Showing: 10951 - 11000 of 23409 results