159,909 results

1. pMK293 (mAID-mCherry2-Hygro)

   Plasmid

   #72831

   Purpose

   C-terminal tagging with mAID-mCherry2 using CRISPR/Cas9

   Depositor

   Masato Kanemaki

   Article

   Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

   Insert

   mAID-mCherry2-Hygro
   

   Use

   CRISPR

   Tags

   mAID-mCherry2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. TOPO mKate2

   Plasmid

   #68441

   Purpose

   TOPO construct expressing mKate2 for generation of GMAP-compatible gene

   Depositor

   Tyler Jacks

   Article

   Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

   Insert

   mKate2
   

   Use

   Gmap

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Plac

   Available since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCB-2

   Plasmid

   #210388

   Purpose

   All-in-one cumate-based inducible CRISPRi plasmid for use in Streptomyces

   Depositor

   Gilles van Wezel

   Article

   Bai et al ACS Synth Biol. 2023 Oct 6. doi: 10.1021

   Inserts

   dCas9
   CymR
   sgRNA cassette
   

   Use

   CRISPR; Integrative vector for use in streptomyces

   Tags

   Expression

   Mutation

   BsaI-flanked RFP cassette inserted in place of sp…

   Promoter

   SP11 and SP30

   Available since

   Jan. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-28b-RfxCas13d-His

   Plasmid

   #141322

   Purpose

   Plasmid for bacterial expression and purification of RfxCas13d protein

   Depositor

   Miguel Angel Moreno-Mateos, Ariel Bazzini

   Article

   Kushawah et al Dev Cell. 2020 Sep 28;54(6):805-817.e7.

   Insert

   Rfx-Cas13d
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. PB-CAG-GFPd2

   Plasmid

   #115665

   Purpose

   Piggybac transposon plasmid for destabilized eGFP (GFPd2)

   Depositor

   Jordan Green

   Article

   Rui et al ACS Appl Mater Interfaces. 2019 Feb 22.

   Insert

   GFPd2
   

   Use

   Synthetic Biology

   Tags

   Destabilization

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Feb. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET15b-pA-Mnase

   Plasmid

   #124883

   Purpose

   To transform BL21-Gold(DE3) and induced by IPTG for His-proteinA-MNase fusion protein.

   Depositor

   Keji Zhao

   Article

   Ku et al Genome Res. 2021 Apr 14. pii: gr.260893.

   Insert

   protein A-MNase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. SpRY-YE1-BE4max

   Plasmid

   #198554

   Purpose

   Expresses human codon-optimized SpRY-YE1-BE4max and blasticidin resistance: EFS promoter-SpRY-YE1-BE4max-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

   Insert

   SpRY-YE1-BE4max
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. mRFP-Lact-C2

   Plasmid

   #74061

   Purpose

   Fluorescent biosensor for phosphatidylserine.

   Depositor

   Sergio Grinstein

   Article

   Yeung et al Science. 2008 Jan 11. 319(5860):210-3.

   Insert

   Lactadherin-C2 (MFGE8 Bovine)
   

   Use

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV FLEX Synaptophysin GFP

   Plasmid

   #137188

   Purpose

   Cre-dependent expression of synaptophysin-GFP

   Depositor

   Matthew Nolan

   Article

   Surmeli et al Neuron. 2016 Nov 23;92(4):929. doi: 10.1

   Insert

   Synaptophysin GFP (Syp Mouse)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCS6-YFP-SMASh

    Plasmid

    #68853

    Purpose

    Expresses SMASh tag fused to YFP at C terminus in mammalian cells

    Depositor

    Michael Lin

    Article

    Chung et al Nat Chem Biol. 2015 Jul 27. doi: 10.1038

    Insert

    YFP-SMASh
    

    Use

    Tags

    SMASh tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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