We narrowed to 2,943 results for: Lox

1. pAAV-nEF-Coff/Fon-Arch3.3-p2a-EYFP

   Plasmid

   #137150

   Purpose

   Intersectional viral expression of Arch3.3-EYFP in cells expressing Flp AND NOT Cre

   Depositor

   INTRSECT 2.0 Project, Karl Deisseroth

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Has Service

   AAV8

   Insert

   Coff/Fon-Arch3.3-p2a-EYFP
   

   Use

   AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

   Tags

   Expression

   Mutation

   n/a

   Promoter

   nEF

   Available since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLP-PIGA3 (pLP305)

   Plasmid

   #196992

   Purpose

   Big-IN Landing Pad plasmid encoding pEF1a-driven PuroR-P2A-hmPIGA expression cassette flanked by heterotypic LoxM/LoxP sites, sleeping beauty ITRs and BsmBI homology arms cloning sites.

   Depositor

   Matthew Maurano

   Article

   Brosh et al Mol Cell. 2023 Apr 6;83(7):1140-1152.e7.

   Type

   Empty backbone

   Use

   Cre/Lox, Mouse Targeting, and Synthetic Biology ;…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   April 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-nEF-Con/Foff 2.0-ChR2-mCherry

   Plasmid

   #137143

   Purpose

   Intersectional viral expression of ChR2-mCherry in cells expressing Cre AND NOT Flp

   Depositor

   INTRSECT 2.0 Project, Karl Deisseroth

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Has Service

   AAV8

   Insert

   Con/Foff 2.0-ChR2-mCherry
   

   Use

   AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

   Tags

   Expression

   Mutation

   H134R

   Promoter

   nEF

   Available since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. HT115_pAAV_hSyn-DiO-SomQuasAr6b_EGFP-P2A-somCheRiff_HA

   Plasmid

   #178827

   Purpose

   Cre-on bicistronic expression of QuasAr6b-based Optopatch under an neuronal promoter

   Depositor

   Adam Cohen

   Article

   Tian et al Nat Methods. 2023 Jul;20(7):1082-1094. d

   Insert

   SomQuasAr6a_EGFP-P2A-somCheRiff_HA
   

   Use

   AAV, Cre/Lox, and Mouse Targeting

   Tags

   EGFP, HA

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   Jan. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJEP315-pAAV-U6SaCas9gRNA(CREB)-CMV-SaCas9-DIO-pA

   Plasmid

   #113692

   Purpose

   U6 SaCas9 gRNA expression cassette containing a gRNA targeting the CREB gene, followed by a CMV driven inverted SaCas9. SaCas9 is floxed to render the system cre-dependent.

   Depositor

   Jonathan Ploski

   Article

   Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

   Inserts

   SaCas9 (NEWENTRY )
   SaCas9 gRNA Cassette (NEWENTRY )
   

   Use

   AAV, CRISPR, Cre/Lox, and Mouse Targeting

   Tags

   NLS

   Expression

   Mutation

   Promoter

   Cytomegalo Virus(CMV)

   Available since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-Ef1a-Coff/Fon-sRGECO

   Plasmid

   #137128

   Purpose

   Intersectional viral expression of sRGECO in cells expressing Flp AND NOT Cre

   Depositor

   INTRSECT 2.0 Project, Karl Deisseroth

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Has Service

   AAV8

   Insert

   Coff/Fon-sRGECO
   

   Use

   AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

   Tags

   Expression

   Mutation

   E217D

   Promoter

   EF1a

   Available since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-Ef1a-Con/Fon/Von GCaMP 6m

   Plasmid

   #137165

   Purpose

   Intersectional viral expression of GCaMP6M in cells expressing Cre AND Flp AND Vcre

   Depositor

   INTRSECT 2.0 Project, Karl Deisseroth

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Insert

   3x-GCaMP6M
   

   Use

   AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

   Tags

   Expression

   Mutation

   Removed RSET tag

   Promoter

   Ef1a

   Available since

   Nov. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-Ef1a-Con/Foff 2.0-BFP

   Plasmid

   #137130

   Purpose

   Intersectional viral expression of BFP in cells expressing Cre AND NOT Flp

   Depositor

   INTRSECT 2.0 Project, Karl Deisseroth

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Has Service

   AAV8

   Insert

   Con/Foff 2.0-BFP
   

   Use

   AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

   Tags

   Expression

   Mutation

   n/a

   Promoter

   EF1a

   Available since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEMS1062

   Plasmid

   #29011

   Purpose

   The plasmid contains the Pleaides (Ple) MiniPromoter Ple155 derived from the human PCP2 gene and designed for expression in ON Bipolar cells of the retina.

   Depositor

   Elizabeth Simpson

   Article

   Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414

   Insert

   Ple155-Cre/EGFP-NLS
   

   Use

   High copy number homologous recombination plasmid…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   MiniPromoter Ple155 from the human PCP2 gene

   Available since

   Feb. 22, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-TRE-flex-ChR2 (E123T/T159C)-EYFP

    Plasmid

    #135176

    Purpose

    AAV expression of TRE(TRE3G)-driven, Cre-dependent, humanized channelrhodopsin E123T/T159C mutant fused to EYFP for optogenetic activation.

    Depositor

    Tetsuo Yamamori

    Article

    Ebina et al Proc Natl Acad Sci U S A. 2019 Nov 5;116

    Inserts

    hChR2
    TRE3G
    

    Use

    AAV and Cre/Lox

    Tags

    EYFP (C terminal on insert)

    Expression

    Mammalian

    Mutation

    E123T and T159C

    Promoter

    TRE3G

    Available since

    Jan. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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