We narrowed to 5,601 results for: Feng Zhang

1. lentiGuide-Puro-Chen-tracrRNA_2xBsmBI

   Plasmid

   #196710

   Purpose

   Backbone for cloning single guides (Chen tracrRNA PMID: 24360272) or guide libraries with untranscribed barcodes and a tracrRNA of choice

   Depositor

   Bernhard Schmierer

   Article

   Schmierer et al Mol Syst Biol. 2017 Oct 9;13(10):945. do

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. AiP942-pX330-Sncg

   Plasmid

   #181774

   Purpose

   CRISPR/Cas9 guide vector

   Depositor

   Tanya Daigle

   Insert

   Sncg guide
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. AiP926-pX330-Batf3

   Plasmid

   #181772

   Purpose

   CRISPR/Cas9 guide vector

   Depositor

   Tanya Daigle

   Insert

   Batf3 guide
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. AiP927-pX330-Chrna6

   Plasmid

   #181765

   Purpose

   CRISPR/Cas9 guide vector

   Depositor

   Tanya Daigle

   Insert

   Chrna6 guide
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. AiP928-pX330-Gpr139

   Plasmid

   #181767

   Purpose

   CRISPR/Cas9 guide vector

   Depositor

   Tanya Daigle

   Insert

   Gpr139 guide
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. AiP938-pX330-Slc17a7

   Plasmid

   #181775

   Purpose

   CRISPR/Cas9 guide vector

   Depositor

   Tanya Daigle

   Insert

   Slc17a7 guide
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. AiP939-pX330-Ctxn3

   Plasmid

   #181769

   Purpose

   CRISPR/Cas9 guide vector

   Depositor

   Tanya Daigle

   Insert

   Ctxn3 guide
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBoBi-hLAMP2-C-GC6s

   Plasmid

   #154151

   Purpose

   To detect the calcium ion releases close to the lysosomal surface

   Depositor

   Sheng-cai Lin

   Article

   Li et al Cell Metab. 2019 Sep 3;30(3):508-524.e12

   Insert

   LAMP2 and GCamp6s (LAMP2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   WT

   Promoter

   CMV

   Available since

   Sept. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. LentiCRISPRv2 RFP670

   Plasmid

   #187646

   Purpose

   lentiviral vector expressing RFP670 alongside Cas9 and an sgRNA cloning site

   Depositor

   Martin Carroll

   Insert

   RFP 670
   

   Use

   Lentiviral

   Tags

   RFP670 and sgRNA cloning site

   Expression

   Mutation

   Promoter

   EFS (P2A)

   Available since

   July 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. lenti.Cas9.BFP.Blast

    Plasmid

    #196714

    Purpose

    WT SpCas9 with BSD-TagBFP for selection and sorting

    Depositor

    Bernhard Schmierer

    Insert

    Cas9-T2A-BSD-TagBFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. eSpCas9(1.1)-T2A-Puro

    Plasmid

    #101039

    Purpose

    Enhanced SpCas9(1.1) with puromycin resistance gene via T2A linker.

    Depositor

    Andrea Németh

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    3xFLAG and T2A-Puro

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    Nov. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLenti_P2A_Blast

    Plasmid

    #211365

    Purpose

    Lentiviral expression vector for an insert of interest linked to a P2A-T2A blasticidin sequence. Produces virus very efficiently. Can also be used for regular transfections

    Depositor

    Jacques Neefjes

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. AAV8R-12 Capsid

    Plasmid

    #213968

    Purpose

    AAV packaging vector carrying AAV2 rep gene and Cap8R modified AAV8 capsid coding sequence

    Depositor

    Zhonghua Lu

    Article

    Chen et al Cell. 2023 Nov 22;186(24):5394-5410.e18.

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. mCherry-pCAG-donor_opt(R2Tg)-GFP(intron)

    Plasmid

    #223249

    Purpose

    Mammalian expression of the optimized R2Tg RNA donor

    Depositor

    Wei Li

    Article

    Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

    Insert

    R2Tg donor_opt
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PA-Dre

    Plasmid

    #169706

    Purpose

    Photoactivatable Dre recombinase for AAV production and expression in mammalian cells

    Depositor

    Dali Li

    Article

    Li et al Proc Natl Acad Sci U S A. 2020 Dec 29;11

    Insert

    Dre246-nMag-T2A-pMag-Dre247
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CAG

    Available since

    May 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. mCherry-pCAG-donor(R2Tg)-GFP(intron)

    Plasmid

    #223248

    Purpose

    Mammalian expression of the R2Tg RNA donor

    Depositor

    Wei Li

    Article

    Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

    Insert

    R2Tg donor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-pCALM1-EYFP-WPRE-bGHpA

    Plasmid

    #213971

    Purpose

    AAV expression of EYFP from pCALM1 promoter

    Depositor

    Zhonghua Lu

    Article

    Wang et al Cell Rep. 2023 Nov 28;42(11):113348. doi

    Insert

    pCALM1
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. AAVS1-pur-CAG-Bi-DREADD

    Plasmid

    #159457

    Purpose

    AAVS1 targeting donor plasmid with Bi-DREADD expression cassette (hM3Dq-mCherry-P2A-HA-KORD) and puromycin selection gene

    Depositor

    Yuejun Chen

    Article

    Xiong et al Cell Stem Cell. 2020 Sep 16. pii: S1934-

    Insert

    Bi-DREADD
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    fusion protein of hM3Dq-mCherry and HA-KORD seper…

    Promoter

    CAG

    Available since

    Dec. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-pCALM1-SpCas9-miniU6-sgRNAShank3

    Plasmid

    #213973

    Purpose

    AAV vector to express SpCas9 driven by pCALM1 promoter for targeting Shank3 locus

    Depositor

    Zhonghua Lu

    Article

    Wang et al Cell Rep. 2023 Nov 28;42(11):113348. doi

    Insert

    Shank3 sgRNA
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-hsyn-GRAB_rDA-mut

    Plasmid

    #140558

    Purpose

    Expresses the red fluorescent DA-insensitive control sensor GRAB_rDA-mut in neurons

    Depositor

    Yulong Li

    Article

    Sun et al Nat Methods. 2020 Nov;17(11):1156-1166.

    Has Service

    AAV9

    Insert

    Red fluorescent DA-insensitive sensor GRAB_rDA-mut (control sensor)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    July 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge