We narrowed to 18,365 results for: Tec

1. pUPD2_miniDFR (GB2566)

   Plasmid

   #160595

   Purpose

   Minimal promoter of slDFR gene containing 62bp upstream the transcription start site and the 5'UTR region

   Depositor

   Diego Orzaez

   Article

   Kallam et al Plant Biotechnol J. 2023 Apr 9. doi: 10.

   Insert

   miniDFR
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pBLO1811_arC9_noNLS_human

   Plasmid

   #74494

   Purpose

   Human codon optimized plasmid to express arC9 t2a mCherry w/o NLS and a sgRNA

   Depositor

   David Savage

   Article

   Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

   Insert

   arC9
   

   Use

   CRISPR

   Tags

   t2a mCherry (C-terminal on insert)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCTCON2-BYG

   Plasmid

   #158126

   Purpose

   Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (wild-type construct)

   Depositor

   James Van Deventer

   Article

   Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

   Insert

   mTagBFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1-10

   Available since

   Jan. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. mCherry-2xCL-YFP-Vpr

   Plasmid

   #105215

   Purpose

   Bi-functional Fluorescent reporter protein to visualize HIV-1 fusion and core-trafficking

   Depositor

   Gregory Melikyan

   Article

   Sood et al J Biol Chem. 2017 Oct 18. pii: M117.8180

   Insert

   Vpr
   

   Use

   Tags

   PC2x (SQNY/IRKVL), eYFP, and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. c3GIC9

   Plasmid

   #62191

   Purpose

   col1a1 targeting vector for inducible [TRE3G]-GFP-IRES-Cas9 expression. Contains NsiI cloning site for U6-sgRNA cassettes

   Depositor

   Lukas Dow

   Article

   Dow et al Nat Biotechnol. 2015 Apr;33(4):390-4. do

   Insert

   humanized S. Pyogenes Cas9
   

   Use

   CRISPR

   Tags

   3x FLAG

   Expression

   Mutation

   Promoter

   TRE3G

   Available since

   Aug. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET-APOBEC1-YTHmut

   Plasmid

   #200159

   Purpose

   For bacterial expression of the APOBEC1-YTHmut fusion protein for protein purification

   Depositor

   Kate Meyer

   Article

   Zhu et al Front Cell Dev Biol. 2022 Apr 27;10:8882

   Insert

   APOBEC1-YTHmut
   

   Use

   Tags

   6xHis, HA, and MBP

   Expression

   Bacterial

   Mutation

   Codon optimized for E coli expression

   Promoter

   LacZ

   Available since

   June 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMM131

   Plasmid

   #127214

   Purpose

   BeYDV replicon with constitutive Luc expression, AtSTM, sgRNA targeting PDS

   Depositor

   Daniel Voytas

   Article

   Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

   Insert

   Luc, AtSTM, sgRNA
   

   Use

   Tags

   Expression

   Plant

   Mutation

   codon optimized CDSs

   Promoter

   Available since

   Dec. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAAP003l2

   Plasmid

   #209748

   Purpose

   RepA(N99D) SC101 T0-BS7 araC pBAD SD8 NpuC-C[AGGA]LFVY-NpuN-SsrA ProK 5 tRNA operon tetA-orfL bla

   Depositor

   Ahmed Badran

   Article

   Costello et al Nat Biotechnol. 2024 Sep 11. doi: 10.103

   Insert

   NpuC-C[AGGA]LFVY-NpuN-SsrA(rc)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMM441

   Plasmid

   #61271

   Purpose

   Also referred to as mRGNndm-1. Expresses Cas9, tracrRNA and a guide RNA which target the NDM-1 gene. Contains an R1162 origin and chloramphenicol resistance. Can be mobilized by S17-1 lambda pir.

   Depositor

   Timothy Lu

   Article

   Citorik et al Nat Biotechnol. 2014 Sep 21. doi: 10.103

   Insert

   Cas9, tracrRNA, crRNA
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET-Cre-6xHis

    Plasmid

    #62939

    Purpose

    Expression of Cre-6xHis in bacterial of cells

    Depositor

    David Liu

    Article

    Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

    Insert

    Cre recombinase
    

    Use

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. ABE8r-V106W

    Plasmid

    #208295

    Purpose

    Expresses ABE8r in mammalian cells

    Depositor

    Weixin Tang

    Article

    Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

    Insert

    TadA8r-V106W-SpCas9 D10A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    W23R, H36L, R47K, P48A, R51L, I76Y, V82S, A106W, …

    Promoter

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. CyTERM-mGFP

    Plasmid

    #62237

    Purpose

    ER targeted transmembrane domain with monomeric EGFP oriented towards the cytoplasm. Used for assessing fluorescent protein oligomerization.

    Depositor

    Erik Snapp

    Article

    Costantini et al Traffic. 2012 May;13(5):643-9. doi: 10.1

    Insert

    CyTERM-mGFP
    

    Use

    Tags

    mGFP

    Expression

    Mammalian

    Mutation

    signal anchor of rabbit cytochrome p450 and monom…

    Promoter

    CMV

    Available since

    March 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBJ7

    Plasmid

    #167136

    Purpose

    A tetracyclin resistant plasmid for Tn7-mediated chromosomal integration of Photorhabdus luminescens luxCDABE operon with Pkan promoter

    Depositor

    Akira Mine

    Article

    Matsumoto et al Plant Commun. 2021 Jul 20;3(1):100227. d

    Insert

    luxCDABE
    

    Use

    Luciferase

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Kanamycin promoter

    Available since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pFastBac TwinStrepII Prescission SNAPf TEV cloning vector with BioBrick PolyPromoter LIC Subcloning (438-SNAP-V1)

    Plasmid

    #55222

    Depositor

    Scott Gradia

    Article

    Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    SNAPf and TwinStrepII

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. A3A-Gam

    Plasmid

    #119769

    Purpose

    Conversion of C to T monocotyledons protoplasts

    Depositor

    Caixia Gao

    Article

    Zong et al Nat Biotechnol. 2018 Oct 1. pii: nbt.426

    Insert

    Gam-APOBEC3A-nCas9-NLS-2XUGI-NLS
    

    Use

    Tags

    NLS (flanking UGI)

    Expression

    Plant

    Mutation

    nCas9 has a mutation D10A in wild type SpCas9

    Promoter

    maize Ubiquitin-1

    Available since

    Sept. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBJ1

    Plasmid

    #167130

    Purpose

    A gentamycin resistant plasmid for Tn7-mediated chromosomal integration of Photorhabdus luminescens luxCDABE operon with Pfrr promoter

    Depositor

    Akira Mine

    Article

    Matsumoto et al Plant Commun. 2021 Jul 20;3(1):100227. d

    Insert

    luxCDABE
    

    Use

    Luciferase

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Frr promoter

    Available since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. MTK Lentivirus Single Cassette Destination Vector (pAN2414)

    Plasmid

    #194220

    Purpose

    Backbone for constructing single casette expression plasmids using the Mammalian Tookit

    Depositor

    Hana El-Samad

    Article

    Weinberg et al bioRxiv 2021.04.20.440678

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAG1280 NLS-mCherry-FIREtag-NES

    Plasmid

    #205172

    Purpose

    expresses NLS-mCherry-FIREtag-NES in mammalian cells

    Depositor

    Arnaud Gautier

    Article

    Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

    Insert

    NLS-mCherry-FIREtag-NES
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Fgfr2-T2A-H2B-Halo donor

    Plasmid

    #113117

    Purpose

    Donor plasmid for knock-in T2A-H2B-Halo to the c-terminal of mouse Fgfr2 coding sequence

    Depositor

    Janet Rossant

    Article

    Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

    Insert

    Fgfr2 donor region including homology arms and T2A-H2B-Halo
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pKB817 (pBra-Hfq)

    Plasmid

    #174661

    Purpose

    Plpp/PlacUV5- directed synthesis of the alpha NTD fused to E. coli Hfq. Useful as three-hybrid positive control.

    Depositor

    Katherine Berry, Ann Hochschild

    Article

    Berry et al Nucleic Acids Res. 2018 Jan 25;46(2):e12

    Insert

    Hfq
    

    Use

    Tags

    alpha NTD

    Expression

    Bacterial

    Mutation

    Promoter

    lpp/lacUV5 (tandem promoter)

    Available since

    May 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge