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We narrowed to 8,434 results for: Dos

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Showing: 4401 - 4420 of 8434 results

  4. pRS424-TUB2 - human TUBB4a Cterminal tail C127S, E435C

    Plasmid
    #60405
    Purpose
    Expression of chimeric yeast beta tubulin (TUB2) - human beta4a tubulin Cterminal tail (TUBB4a) C127S, E435C for crosslinking polyglutamate peptide
    Depositor
    Ron Vale
    Article
    Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do
    Insert
    TUB2
    Expression
    Yeast
    Mutation
    TUB2 amino acids 429 - end, replaced with human T…
    Promoter
    GAL
    Available Since
    Jan. 26, 2015
    Availability
    Academic Institutions and Nonprofits only

  8. pDG459

    Plasmid
    #100901
    Purpose
    SpCas9 with 2A-Puro and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.
    Depositor
    Paul Thomas
    Article
    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do
    Inserts
    humanized CRISPR associated protein 9
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    Use
    CRISPR and Mouse Targeting
    Tags
    3xFLAG and T2A-PuroR
    Expression
    Mammalian
    Promoter
    CBh and U6
    Available Since
    Dec. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    100901-plasmid-map-sequence-id-197197

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  13. lentiCRISPR_DKO

    Plasmid
    #183192
    Purpose
    Plasmid with Cas9, two U6 promoters and two gRNA scaffolds that allows for inserting two gRNAs for combinatorial CRISPR Screen or double-knock-out (DKO) screen
    Depositor
    Lang Li
    Article
    Tang et al STAR Protoc. 2022 Aug 24;3(3):101556. do
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    hU6, mU6
    Available Since
    Aug. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    183192-plasmid-map-sequence-id-360098

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  20. mCherry-DRP1 K38A

    Plasmid
    #232350
    Purpose
    Expresses K38A variant of human DRP1 with mCherry tag
    Depositor
    Samantha Lewis
    Insert
    DRP1 (DNM1L Human)
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    K38A
    Promoter
    CMV
    Available Since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    232350-plasmid-map-sequence-id-462165

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Showing: 4401 - 4420 of 8434 results