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pAAV-EFS-DIO-GRAB_Ado1.0

Plasmid

#153291

Purpose

Express the adenosine sensor GRAB_Ado1.0 in Cre positive cells

Depositor

Yulong Li

Article

Peng et al Science. 2020 Sep 4;369(6508). pii: 369/

Insert

Adenosine sensor GRAB_Ado1.0

Use

AAV

Promoter

EFS

Available Since

July 30, 2020

Availability

Academic Institutions and Nonprofits only

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MXS_E2-Crimson

Plasmid

#62410

Purpose

MXS_chaining vector with E2-Crimson

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

E2-Crimson

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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BglB in pET29b(+)

Plasmid

#55773

Purpose

Expresses beta-glucosidase

Depositor

Justin Siegel

Article

Carlin et al PLoS One. 2016 Jan 27;11(1):e0147596. do

Insert

Beta-glucosidase B (bglB Synthetic)

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

July 30, 2014

Availability

Academic Institutions and Nonprofits only

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pBS Notch4HA

Plasmid

#21470

Depositor

Jan Kitajewski

Article

Uyttendaele et al Dev Biol. 1998 Apr 15. 196(2):204-17.

Insert

Notch4 (Notch4 Mouse)

Use

Cloning vector

Tags

HA

Available Since

Aug. 14, 2009

Availability

Academic Institutions and Nonprofits only
p70a-T7RNAP

Plasmid

#105683

Purpose

Expresses T7 polymerase in cell-free TXTL.

Depositor

Chase Beisel

Article

Maxwell et al Methods. 2018 Jul 1;143:48-57. doi: 10.1

Insert

T7 polymerase

Expression

Bacterial

Promoter

OR2-OR1-Pr (bacteriophage Lambda with one mutatio…

Available Since

May 19, 2021

Availability

Academic Institutions and Nonprofits only

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LeGO-Luc2

Plasmid

#154006

Purpose

Lentiviral vector expressing firefly luciferase (Luc2)

Depositor

Boris Fehse

Article

Ellinger et al Viruses. 2020 May 25;12(5). pii: v120505

Insert

Firefly Luciferase

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV

Available Since

July 22, 2020

Availability

Academic Institutions and Nonprofits only

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mCherry-CEPT1

Plasmid

#119079

Purpose

Visualize localization of ER enzyme CEPT1, which converts diacylglycerol precursors to phosphatidylethanolamine or phosphatidylcholine

Depositor

Gia Voeltz

Article

English et al Nat Cell Biol. 2013 Feb;15(2):169-78. do

Insert

CEPT1 (CEPT1 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 20, 2018

Availability

Academic Institutions and Nonprofits only

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STIMATE-mCherry

Plasmid

#70676

Purpose

Expresses STIMATE or TMEM110 in mammalian cells

Depositor

Yubin Zhou

Article

Jing et al Nat Cell Biol. 2015 Oct;17(10):1339-47.

Insert

STIMATE (TMEM110 Human)

Tags

mcherry

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only
hV1b-GFP-1

Plasmid

#67847

Purpose

mammalian expression of human V1b receptor with GFP tag

Depositor

Christian Gruber

Article

Koehbach et al Proc Natl Acad Sci U S A. 2013 Dec 24;11

Insert

V1b receptor (AVPR1B Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 15, 2015

Availability

Academic Institutions and Nonprofits only
pLKO-shTRAF2#1

Plasmid

#44127

Depositor

William Hahn

Article

Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

Insert

TRAF2 (TRAF2 Human)

Use

Lentiviral and RNAi

Available Since

March 25, 2013

Availability

Academic Institutions and Nonprofits only
human p53-(1-360)

Plasmid

#24865

Depositor

Cheryl Arrowsmith

Article

Ayed et al Nat Struct Biol. 2001 Sep . 8(9):756-60.

Insert

p53 (TP53 Human)

Tags

His

Expression

Bacterial

Mutation

Contains aa 1-360.

Available Since

Aug. 20, 2010

Availability

Academic Institutions and Nonprofits only
HA-GABARAPL2

Plasmid

#137761

Purpose

Expression of GABARAPL2

Depositor

Carol Mercer

Article

Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

Insert

GABARAPL2 (GABARAPL2 Human)

Tags

HA and myc

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-HA-mIFITM3

Plasmid

#58389

Purpose

Expressed murine IFITM3 with an N-terminal HA tag in mammalian cells

Depositor

Howard Hang, Jacob Yount

Article

Yount et al Nat Chem Biol. 2010 Aug;6(8):610-4. doi:

Insert

IFITM3 (Ifitm3 Mouse)

Tags

HA

Expression

Mammalian

Promoter

CMV IE

Available Since

Sept. 3, 2014

Availability

Academic Institutions and Nonprofits only
pME-Gal80ts

Plasmid

#51619

Purpose

vertebrate expression of full-length temperature-sensitive Gal80 with stop

Depositor

Joshua Bonkowsky

Article

Fujimoto et al Dev Dyn. 2011 Oct;240(10):2324-34. doi:

Insert

Gal80ts (GAL80 Budding Yeast)

Use

Vertebrate expression

Mutation

temperature sensitive (G203V, G323R)*

Available Since

March 27, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV5B-Flag-Smad1

Plasmid

#11735

Depositor

Jeff Wrana

Article

Hoodless et al Cell. 1996 May 17. 85(4):489-500.

Insert

SMAD1 (SMAD1 Human)

Tags

Flag

Expression

Mammalian

Available Since

May 12, 2006

Availability

Industry, Academic Institutions, and Nonprofits
FLAG-Skp2

Plasmid

#89142

Purpose

mammalian expression of human Skp2 with N-terminal FLAG tag

Depositor

Elizabeth Wilson

Article

Su et al Mol Cell Endocrinol. 2017 Jan 5;439:1-9.

Insert

Skp2 (SKP2 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

April 21, 2017

Availability

Academic Institutions and Nonprofits only

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pSMP-G9A_1

Plasmid

#36395

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

G9A (EHMT2 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
RCAS (A) Six1 (CT#664)

Plasmid

#13876

Depositor

Cliff Tabin

Article

Heanue et al Genes Dev. 1999 Dec 15. 13(24):3231-43.

Insert

Six1 (Six1 Mouse)

Use

Retroviral; Avian expression

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
AICSDP-45: TTN-mEGFP

Plasmid

#114412

Purpose

Homology arms and linker-mEGFP sequence for C-terminus tagging of human TTN, via Cas9-excisable CAGGS-mCherry selection cassette

Depositor

Allen Institute for Cell Science

Article

Roberts et al Stem Cell Reports. 2019 May 14;12(5):114

Insert

TTN Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (TTN Human)

Use

CRISPR; Donor template

Tags

linker-mEGFP

Expression

Mammalian

Mutation

homology arms contain point mutations to disrupt …

Available Since

Sept. 5, 2018

Availability

Academic Institutions and Nonprofits only

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plko-shmfn2-mito-GFP-ER tethering

Plasmid

#160509

Purpose

Rescue mfn2 knockdown by tethering mitochondria and ER with GFP

Depositor

Qing Deng

Article

Zhou et al J Cell Sci. 2020 Sep 4;133(17). pii: jcs

Insert

mito-GFP-ER tethering

Use

Lentiviral

Promoter

CMV

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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