We narrowed to 39,261 results for: lat

1. pMRXIP-GFP-1K8Q

   Plasmid

   #221742

   Purpose

   Stable expression of GFP-1K8Q in mammalian cells to monitor membrane charge

   Depositor

   Noboru Mizushima

   Article

   Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

   Insert

   C -terminal region of KRAS (KRAS Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   deleted amino acids 1-170, mutated K to Q

   Promoter

   Available since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMRXIP-GFP-3K6Q

   Plasmid

   #221741

   Purpose

   Stable expression of GFP-3K6Q in mammalian cells to monitor membrane charge

   Depositor

   Noboru Mizushima

   Article

   Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

   Insert

   C -terminal region of KRAS (KRAS Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   deleted amino acids 1-170, mutated K to Q

   Promoter

   Available since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMRXIP-GFP-5K4Q

   Plasmid

   #221740

   Purpose

   Stable expression of GFP-5K4Q in mammalian cells to monitor membrane charge

   Depositor

   Noboru Mizushima

   Article

   Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

   Insert

   C -terminal region of KRAS (KRAS Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   deleted amino acids 1-170, mutated K to Q

   Promoter

   Available since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMXS-IRES-BLAST flag-CAD M1601E

   Plasmid

   #169828

   Purpose

   Expresses C-terminal flag-tagged CAD with mutation at reported dimerization interface of the DHOase domain

   Depositor

   Richard Possemato

   Article

   Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

   Insert

   CAD (CAD Human)
   

   Use

   Retroviral

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   M1601E; TCCC -> AGTC silent mutations at nt527…

   Promoter

   Available since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-TNT4-TadA8e-SpRY N aa2-713-InteinN-miR122 TS

   Plasmid

   #209787

   Purpose

   Expresses TadA8e and SpRY cas9N by the specific TNT4 promoter and incorporation of the miR122 target sequences (miR122TS) into the 3’ untranslated region (3’ UTR)

   Depositor

   Yuxuan Guo

   Article

   Yang et al Circulation. 2024 May 28;149(22):1778-17

   Insert

   TNT4, TadA8e, SpRY N, inteinN, miR122 TS
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   TNT4

   Available since

   May 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3(+)-UFBP1 F196A V198A-MYC

   Plasmid

   #204556

   Purpose

   Expresses FLAG-tagged UFBP1 F196A V198A in mammalian cells

   Depositor

   Masaaki Komatsu

   Article

   Ishimura et al Nat Commun. 2022 Dec 21;13(1):7857. doi:

   Insert

   UFBP1 (DDRGK1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   F196A V198A

   Promoter

   Available since

   Aug. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAsh-MCL-LC3B

   Plasmid

   #204573

   Purpose

   Expresses Ash-tagged LC3B in mammalian cells

   Depositor

   Masaaki Komatsu

   Article

   Ishimura et al Nat Commun. 2022 Dec 21;13(1):7857. doi:

   Insert

   LC3B (MAP1LC3B Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   delta121-125aa

   Promoter

   Available since

   Aug. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHis-3xFLAG-CMV14-CYB5R3 K214R

   Plasmid

   #204552

   Purpose

   Expresses 3xFLAG-tagged CYB5R3 K214R in mammalian cells

   Depositor

   Masaaki Komatsu

   Article

   Ishimura et al Nat Commun. 2022 Dec 21;13(1):7857. doi:

   Insert

   CYB5R3 (CYB5R3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   K214R

   Promoter

   Available since

   Aug. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pENeCFPCNAL2 pc922

   Plasmid

   #167563

   Purpose

   For construction of CFP-tagged human PCNA, the GFP coding sequence in the pENeGFPCNAL2 vector was replaced by the ECFP coding sequence from the pECFP-C1 vector (Clontech Laboratories, Inc., CA).

   Depositor

   Cristina Cardoso

   Article

   Zhang et al Nucleic Acids Res. 2017 Apr 25. doi: 10.

   Inserts

   Proliferating Cell Nuclear Antigen (PCNA Human)
   CFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSUMO mouse METAP1 1-79

    Plasmid

    #197397

    Purpose

    Expresses SUMO-tagged mouse METAP1, residues 1-79

    Depositor

    David Giedroc

    Article

    Weiss et al Cell. 2022 Jun 9;185(12):2148-2163.e27.

    Insert

    METAP1 1-79 (Metap1 Mouse)
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSUMO mouse METAP1 1-59

    Plasmid

    #197396

    Purpose

    Expresses SUMO-tagged mouse METAP1, residues 1-59

    Depositor

    David Giedroc

    Article

    Weiss et al Cell. 2022 Jun 9;185(12):2148-2163.e27.

    Insert

    METAP1 1-59 (Metap1 Mouse)
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. Coexpressed Free deadFPPS-NES

    Plasmid

    #182492

    Purpose

    Yeast integrative plasmid for expressing ERG20 (GAL10 promoter) and fusion protein deadFPPS-AcNES1 (GAL7 promoter). deadFPPS is ERG20(K197G-K254A), an inactive mutant of ERG20.

    Depositor

    Claudia Vickers

    Article

    Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.

    Inserts

    FPPS
    deadFPPS-NES
    

    Use

    Synthetic Biology; Metabolic engineering

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL10 and GAL7

    Available since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pT7-EGFP-C1-HseIF4G1-L640AV646A_AD

    Plasmid

    #148520

    Purpose

    Mammalian Expression of HseIF4G1-L640AV646A

    Depositor

    Elisa Izaurralde

    Article

    Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

    Insert

    HseIF4G1-L640AV646A (EIF4G1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    one non silent mutation M432V (natural variant is…

    Promoter

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pT7-EGFP-C1-HseIF4G1-LIVVtoAAAA_AD

    Plasmid

    #148521

    Purpose

    Mammalian Expression of HseIF4G1-LIVVtoAAAA

    Depositor

    Elisa Izaurralde

    Article

    Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

    Insert

    HseIF4G1-LIVVtoAAAA (EIF4G1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    one non silent mutation M432V (natural variant is…

    Promoter

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pT7-EGFP-C1-HseIF4G1-Y619AL624AL625A_AD

    Plasmid

    #148522

    Purpose

    Mammalian Expression of HseIF4G1-Y619AL624AL625A

    Depositor

    Elisa Izaurralde

    Article

    Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

    Insert

    HseIF4G1-Y619AL624AL625A (EIF4G1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    one non silent mutation M432V (natural variant is…

    Promoter

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCAGGS-chGrb2/eDHFR(69K6)-iRFP713

    Plasmid

    #178854

    Purpose

    Mammalian expression of Grb2-eDHFR(69K6) chimera fused to iRFP713

    Depositor

    Shinya Tsukiji

    Article

    Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

    Insert

    Grb2(1-59)-eDHFR(69K6)-Grb2(152-216)-iRFP713 (GRB2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Grb2: Y160E

    Promoter

    CAG

    Available since

    July 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPBpuro-miRFP670-eDHFR(69K6)-G-alpha-s

    Plasmid

    #172105

    Purpose

    Mammalian expression of Gαs fused to miRFP670-eDHFR(69K6)

    Depositor

    Shinya Tsukiji

    Article

    Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

    Insert

    miRFP670-eDHFR(69K6)-Gαs (GNAS Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Gαs: C3S

    Promoter

    CAG

    Available since

    July 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pmirGlo-RBM8A-3'UTRAluY-Sz mut 2598-4066

    Plasmid

    #171135

    Purpose

    reporter

    Depositor

    Emanuela Felley-Bosco

    Article

    Abukar et al Cells. 2021 Dec 15;10(12). pii: cells101

    Insert

    RBM8A 3'UTR (RBM8A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A3302G, A3356G, A3373G, A3389G,A3451G, T3729C, A3…

    Promoter

    Available since

    Dec. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. px458-AMBRA1 #3

    Plasmid

    #172607

    Purpose

    Expresses gRNA #3 against AMBRA1 and Cas9 from S. pyogenes with 2A-EGFP

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    gRNA against AMBRA1 (AMBRA1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pENTR223-PARK7H126A

    Plasmid

    #115180

    Purpose

    Gateway cloning of PARK7H126A into any destination vector

    Depositor

    Mohan Babu

    Article

    Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

    Insert

    PARK7H126A (PARK7 Human)
    

    Use

    Gateway cloning into appropriate destination vect…

    Tags

    Expression

    Mutation

    p.H126A

    Promoter

    Available since

    March 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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