We narrowed to 7,208 results for: ef1a;

1. IDP378

   Plasmid

   #87005

   Purpose

   plasmid for cell culture expression of SYNseq components

   Depositor

   Anthony Zador

   Article

   Peikon et al Nucleic Acids Res. 2017 Jul 7;45(12):e11

   Insert

   preLib-bfp-4xboxB
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Feb. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLX_311-KRAB-dCas9

   Plasmid

   #96918

   Purpose

   for CRISPRi, lentiviral expression of KRAB-dCas9 and BlastR. Also called pXPR_121

   Depositor

   John Doench, David Root, William Hahn

   Article

   Rosenbluh et al Nat Commun. 2017 May 23;8:15403. doi: 10

   Insert

   dCas9
   

   Use

   Lentiviral

   Tags

   KRAB

   Expression

   Mammalian

   Mutation

   dCas9

   Promoter

   EF1a

   Available Since

   June 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. SadCas9 KRAB

   Plasmid

   #188504

   Purpose

   Expresses FLAG tagged SadCas9 KRAB

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A/N580A

   Promoter

   EF1a

   Available Since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. Lenti-miniTurbo-NLS-Blast

   Plasmid

   #232347

   Purpose

   Lentiviral control vector expressing miniTurboNLS without dCas9

   Depositor

   Ali Shilatifard

   Article

   Cenik et al Mol Cell. 2024 Dec 19;84(24):4929-4944.e

   Insert

   miniTurbo
   

   Use

   Lentiviral

   Tags

   3xHA and 3xSV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMA3790

   Plasmid

   #70110

   Purpose

   mtDNA elimination/production of rho-0 cells

   Depositor

   Mikhail Alexeyev

   Article

   Spadafora et al PLoS One. 2016 May 2;11(5):e0154684. doi

   Inserts

   EGFP
   hUNG1 Y147A
   Neo
   

   Use

   Tags

   None, human OTC mitochondrial targeting sequence,…

   Expression

   Mammalian

   Mutation

   Y147A, aa's 1-77 removed

   Promoter

   CMV, EF1a, and SV40 and native

   Available Since

   May 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSBbi_RP_MicroID2-HA

   Plasmid

   #188521

   Purpose

   Encodes the biotin proximity labeling enzyme MicroID2 for expression and stable integration into mammalian cells

   Depositor

   James Londino

   Article

   Johnson et al Mol Cell Proteomics. 2022 Jun 7:100256.

   Insert

   MicroID2
   

   Use

   Transposon mediated gene integration

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a/RPBSA

   Available Since

   Sept. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. dCas12j3 VPR

   Plasmid

   #188517

   Purpose

   Expresses FLAG tagged dCas12j3 VPR

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   dCas12j3
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D413A

   Promoter

   EF1a

   Available Since

   Nov. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. PB-TO-oNGN2

   Plasmid

   #198397

   Purpose

   Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression. oNGN2 is a Phospho-mutant which avoids inhibition by cdk kinase, NGN2 optimization aided by IDT tool.

   Depositor

   iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

   Inserts

   NEUROG2 (phospho mutant, codon optimized) (NEUROG2 Human)
   puroR-T2A-mycNLS-mTagBFP2
   rtTA3G
   

   Use

   Tags

   T2A-mycNLS-mTagBFP2

   Expression

   Mammalian

   Mutation

   S24A, S193A, S207A, S209A, S219A, S232A, S239A, S…

   Promoter

   CAG, EF1a, and TRE3G

   Available Since

   April 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. lenti-mTagBFP2

   Plasmid

   #216126

   Purpose

   Lentiviral vector for expressing mTagBFP2.

   Depositor

   Stefan Kubicek

   Article

   Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d

   Insert

   EF1a-mTagBFP2-WPRE
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. lenti-tmpknot-epegRNA-puro backbone

    Plasmid

    #214089

    Purpose

    Lentiviral backbone plasmid to insert epegRNAs (mpknot) of interest. The cloning site is BsmbI.

    Depositor

    Ting Zhou

    Article

    Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6, EF1a

    Available Since

    June 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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