We narrowed to 14,055 results for: flag

1. pcDNA3.1-cox8-iPASnFR-L-mScarlet3-3xFLAG

   Plasmid

   #225240

   Purpose

   Mammalian expression of iPASnFR-L in mitochondria

   Depositor

   Loren Looger

   Article

   Tamura et al bioRxiv 2024.08.21.609037

   Insert

   iPASnFR-L
   

   Use

   Tags

   3xFLAG and mScarlet3

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-RIPK3[S199D][S227D]-FLAG

   Plasmid

   #199324

   Purpose

   double mutated phosphorylation site (kinase acitivity and MLKL interaction active)

   Depositor

   Adrian Press

   Article

   Hoff et al Cell Death Dis. 2023 Apr 18;14(4):275. d

   Insert

   Receptor-interacting serine/threonine-protein kinase 3 (RIPK3 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   changed Serine 199 and 227 to Aspartic Acid

   Promoter

   Available Since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-2xFLAG-2xSTREP (empty vector)

   Plasmid

   #172604

   Purpose

   Empty vector for the cloning of an N-terminal 2xFLAG-2xSTREP-tagged gene of interest, and expression in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Type

   Empty backbone

   Use

   Tags

   2xFLAG-2xSTREP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q36

   Plasmid

   #111745

   Purpose

   Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells

   Depositor

   Cheryl Arrowsmith

   Article

   Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

   Insert

   HTT (HTT Human)
   

   Use

   Baculovirus expression

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Q36 (polyQ repeat)

   Promoter

   CMV and P10

   Available Since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pWZL-Neo-Myr-Flag-DEST

   Plasmid

   #15300

   Purpose

   a Gateway-compatible retroviral destination vector which adds a myristoylation sequence and a FLAG tag to each introduced ORF

   Depositor

   Jean Zhao

   Article

   Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   Flag and Myr

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLVX-TetOne-Puro-mitoLbNOX-FLAG

   Plasmid

   #218667

   Purpose

   pLVX-TetOne-Puro-mitoLbNOX-FLAG

   Depositor

   Valentin Cracan

   Article

   Pan et al Nat Chem Biol. 2023 Oct 26. doi: 10.1038

   Insert

   mitoLbNOX
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   FLAG and MTS

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q60

   Plasmid

   #111749

   Purpose

   Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells

   Depositor

   Cheryl Arrowsmith

   Article

   Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

   Insert

   HTT (HTT Human)
   

   Use

   Baculovirus expression

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Q60 (polyQ repeat)

   Promoter

   CMV and P10

   Available Since

   Nov. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. MSCV-Flag-ER-Hoxb8-IRES-GFP

   Plasmid

   #222292

   Purpose

   Less commonly used plasmid for generating ER-Hoxb8 conditionally immortalized myeloid cell lines.

   Depositor

   David Sykes, Mark Kamps

   Article

   Wang et al Nat Methods. 2006 Apr;3(4):287-93.

   Insert

   ER-Hoxb8 (ESR1, Hoxb8 Human, Mouse)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   The estrogen receptor hormone binding domain cont…

   Promoter

   Available Since

   Nov. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. RMCE halo-flag DNA-PKcs

   Plasmid

   #233918

   Purpose

   Low copy number provides expression of human DNA-PKcs with an N-terminal halo-flag tag.

   Depositor

   Katheryn Meek

   Article

   Buehl et al Mol Cell. 2023 Mar 2;83(5):698-714.e4. d

   Insert

   human DNA-PKcs (PRKDC Human)
   

   Use

   Tags

   flag-halo

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti-CMV-SMCHD1-Exon1-48-3xFLAG

    Plasmid

    #130675

    Purpose

    Expresses full-length human SMCHD1 with a C-terminal 3x FLAG tag

    Depositor

    Stephen Tapscott

    Article

    Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

    Insert

    SMCHD1 (SMCHD1 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLVX-Flag-CDK8-WT-IRES-mCherry

    Plasmid

    #68856

    Purpose

    Expresses Human CDK8 Wild-Type

    Depositor

    Matthew Shair

    Article

    Pelish et al Nature. 2015 Sep 28. doi: 10.1038/nature

    Insert

    cyclin-dependent kinase 8 (CDK8 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1α

    Available Since

    March 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLVX-TetOne-Puro-EcSTH-FLAG

    Plasmid

    #218628

    Purpose

    This plasmid contains human codon optimized sequence of EcSTH which is a soluble transhydrogenase from E. coli that can be used to increase NADH/NAD+ ratio in mammalian cells.

    Depositor

    Valentin Cracan

    Article

    Pan et al Nat Chem Biol. 2023 Oct 26. doi: 10.1038

    Insert

    EcSTH
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q145

    Plasmid

    #111731

    Purpose

    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells

    Depositor

    Cheryl Arrowsmith

    Article

    Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

    Insert

    HTT (HTT Human)
    

    Use

    Baculovirus expression

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Q145 (polyQ repeat)

    Promoter

    CMV and P10

    Available Since

    Nov. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLVX-TetOne-Puro-LbNOX-FLAG

    Plasmid

    #218631

    Purpose

    This plasmid contains LbNOX under doxycycline inducible control.

    Depositor

    Valentin Cracan

    Article

    Pan et al Nat Chem Biol. 2023 Oct 26. doi: 10.1038

    Insert

    LbNOX
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-N-Flag-NLRP3 711-1034

    Plasmid

    #75141

    Purpose

    Expresses N-terminal Flag-tagged amino acids 711-1034 of NLRP3 (the LRR domain) in mammalian cells

    Depositor

    Bruce Beutler

    Article

    Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

    Insert

    NLR family, pyrin domain containing 3 (Nlrp3 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    fragment encoding amino acids 711-1034

    Promoter

    CMV

    Available Since

    May 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEEF1D-FLAG (pcDNA 3.1+) LG6-1

    Plasmid

    #37365

    Depositor

    David Litchfield

    Article

    Gyenis et al J Proteome Res. 2011 Nov 4;10(11):4887-9

    Insert

    EEF1D (EEF1D Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 17, 2012

    Availability

    Academic Institutions and Nonprofits only

17. pLVX-Flag-MIB1-IRES-Puro

    Plasmid

    #140240

    Purpose

    Lentiviral vector expressing flag-tagged MIB1

    Depositor

    Urs Greber

    Article

    Bauer et al Cell Rep. 2019 Dec 17;29(12):3785-3795.e

    Insert

    Flag-MIB1 (MIB1 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pME-mCherry-FLAG-CD59-GPI

    Plasmid

    #50378

    Purpose

    Express mCherry and FLAG-tagged human CD59, a GPI-AP in mammalian cells

    Depositor

    Reika Watanabe

    Article

    Rivier et al Traffic. 2010 Aug;11(8):1017-33. doi: 10

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SR alpha

    Available Since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pLVX-TetOne-Puro-mitoEcSTH-FLAG

    Plasmid

    #218629

    Purpose

    This plasmid contains human codon optimized sequence of mitoEcSTH which is a soluble transhydrogenase from E. coli that can be used to increase NADH/NAD+ ratio in mammalian cells.

    Depositor

    Valentin Cracan

    Article

    Pan et al Nat Chem Biol. 2023 Oct 26. doi: 10.1038

    Insert

    mitoEcSTH
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    FLAG and MTS

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pME-3x-FLAG-Ubiquitin (JDW 1098)

    Plasmid

    #224513

    Purpose

    A Gateway compatible middle entry clone containing a 3x FLAG tagged human Ubiquitin for monitoring ubiquitylation status

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    3x-FLAG-Ubiquitin
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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