We narrowed to 9,820 results for: 158

1. pSLS.416

   Plasmid

   #187207

   Purpose

   Expresses retron Eco1 ncRNA v35 (long a1/a2), and Cas1 + Cas2

   Depositor

   Seth Shipman

   Article

   Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:

   Inserts

   Retron Eco1 ncRNA v35 (long a1/a2)
   Cas1 + Cas2
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7/Lac

   Available since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. FKBP-CD28TMD-NTEVp(H75S)

   Plasmid

   #137828

   Purpose

   MESA split-TEV protease chain with FKBP Rapamycin ectodomain, CD28 transmembrane domain, N-terminal H75S mutant TEV protease

   Depositor

   Joshua Leonard

   Article

   Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

   Insert

   FKBP-CD28TMD-NTEVp(H75S)
   

   Use

   Tags

   3x FLAG

   Expression

   Mammalian

   Mutation

   Mutated Histidine 75 to Serine in NTEVp fragment

   Promoter

   CMV

   Available since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. dCas9-NLS-gs10-cGR2176

   Plasmid

   #188258

   Purpose

   Plasmid ensures constitutive expression of the C-terminal fragment of split GR2 (aa 239-267), fused to the catalytically inactive Cas9 (dCas9) protein, a flexible GS linker and the SV40 NLS signal at the N-terminus

   Depositor

   Roman Jerala

   Article

   Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038

   Insert

   cGR2176
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. piGECI-NES-N1

   Plasmid

   #160422

   Purpose

   Expression of a near-infrared genetically encoded calcium indicator iGECI with a nuclear export signal sequence

   Depositor

   Vladislav Verkhusha

   Article

   Shemetov et al Nat Biotechnol. 2021 Mar;39(3):368-377.

   Insert

   iGECI-NES
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBEST_OR2-OR1-Pr-UTR1_ClpP-T500

   Plasmid

   #153302

   Purpose

   Expresses ClpP protease in the cell-free TX-TL system

   Depositor

   Mikhail Shapiro

   Article

   Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d

   Insert

   clpP (clpP Escherichia coli)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. K401-iLid

   Plasmid

   #122484

   Purpose

   Component of optically-induced motor dimerization system

   Depositor

   Matt Thomson

   Article

   Ross et al Nature. 2019 Aug;572(7768):224-229. doi:

   Insert

   Kinesin 1-401 iLid His tag
   

   Use

   Tags

   His6 and iLid

   Expression

   Bacterial

   Mutation

   Kinesin amino acids 1-401

   Promoter

   T7

   Available since

   Sept. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. TaRGET-ABE-C3.1 ver4.0

   Plasmid

   #188250

   Purpose

   Hypercompact adenine base editors

   Depositor

   Yong-Sam Kim

   Article

   Kim et al Nat Chem Biol. 2022 Sep;18(9):1005-1013.

   Insert

   TaRGET-ABE3.1
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCMV-NLS-FRB-VP16

   Plasmid

   #210515

   Purpose

   expresses NLS-FRB-VP16 component in mammalian cells

   Depositor

   Dongmin Lee

   Article

   Cui et al Nat Chem Biol. 2024 Mar;20(3):353-364. d

   Insert

   NLS-FRB-VP16
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. FRB-CD28TMD-CTEVp(WT)-CS(M)-tTA

   Plasmid

   #137827

   Purpose

   MESA split-TEV protease chain with FRB Rapamycin ectodomain, CD28 transmembrane domain, C-terminal WT TEV protease, M cleavage sequence, and tTA transcription factor

   Depositor

   Joshua Leonard

   Article

   Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

   Insert

   FRB-CD28TMD-CTEVp(WT)-CS(M)-tTA
   

   Use

   Tags

   3x FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEB2-mNeptune2

    Plasmid

    #104012

    Purpose

    Plasmid encoding mNeptune2

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    mNeptune2
    

    Use

    Low copy

    Tags

    Expression

    Bacterial

    Mutation

    Mutations relative to eqFP578 (MSKGEE LIKENM… R32…

    Promoter

    proC

    Available since

    Dec. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. FKBP_cPPVp

    Plasmid

    #118967

    Purpose

    Expresses C-terminus of plum pox virus protease (PPVp) fused to the FK-506 binding protein (FKBP)

    Depositor

    Roman Jerala

    Article

    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

    Insert

    split cPPVp in fusion with FKBP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. FRB_nPPVp

    Plasmid

    #118966

    Purpose

    Expresses N-terminus of plum pox virus protease (PPVp) fused to FKBP-rapamycin binding (FRB) domain

    Depositor

    Roman Jerala

    Article

    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

    Insert

    split nPPVp in fusion with FRB
    

    Use

    Tags

    Myc tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCH68 (dAsCas12a-3xKRAB piggyBac)

    Plasmid

    #217334

    Purpose

    piggyBac expression of dAsCas12a-3xKRAB

    Depositor

    Luke Gilbert

    Article

    Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

    Insert

    dAsCas12a
    

    Use

    CRISPR

    Tags

    nucleoNLS-3xKRAB-3xHA-P2A-TagBFP2

    Expression

    Mammalian

    Mutation

    E993A

    Promoter

    CAG

    Available since

    March 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMRX-IP/Venus-mDFCP1

    Plasmid

    #58742

    Purpose

    Expresses Venus-mDFCP1 in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Koyama-Honda et al Autophagy. 2013 Oct;9(10):1491-9. doi: 1

    Insert

    ZFYVE1 zinc finger, FYVE domain containing 1 (Zfyve1 Mouse)
    

    Use

    Retroviral

    Tags

    Venus

    Expression

    Mutation

    Promoter

    Available since

    Dec. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pHR- flag-Lag16-DLL1

    Plasmid

    #205201

    Purpose

    This vector encodes of the synCAM (DLL-1) and GFP nanobody LAG16

    Depositor

    Wendell Lim

    Article

    Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

    Insert

    synCAM DLL1 and Lag16 anti-GFP nanobody
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pHR- flag-Lag16-MUC4

    Plasmid

    #205198

    Purpose

    This vector encodes of the synCAM (MUC4) and GFP nanobody LAG16

    Depositor

    Wendell Lim

    Article

    Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

    Insert

    synCAM MUC4 and Lag16 anti-GFP nanobody
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. CP1028-ABE8e-dimer

    Plasmid

    #138498

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    ecTadA(WT)-ecTadA(8e)-nSpCas9 (CP1028)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PML-ER-pBabe

    Plasmid

    #73556

    Purpose

    vector encoding a fusion protein between PML-IV and ER (estrogen receptor)

    Depositor

    Gerardo Ferbeyre

    Article

    Acevedo et al Cancer Res. 2016 Jun 1;76(11):3252-64. d

    Insert

    PML-IV (PML Human)
    

    Use

    Retroviral

    Tags

    ER

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    Aug. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pCR1063

    Plasmid

    #111091

    Purpose

    Expresses dCas9-KRAB tagged with mCherry: pHR-hEF1a-dCas9-mcherry-KRAB-mPGK-Hyg

    Depositor

    Jacob Corn

    Article

    Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

    Insert

    dCas9-KRAB
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    Available since

    Oct. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBR322-Pdp1_NTD-ZFN_L-HiBiT

    Plasmid

    #198319

    Purpose

    Loads HiBiT-tagged L fragment of a zinc finger nuclease specific for human AAVS1 locus using Pdp1 packaging domain

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

    Insert

    Pdp1_NTD-ZFN_L-HiBiT
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. Lenti-evoA1-seBEC

    Plasmid

    #174702

    Purpose

    Small-molecule controlled base editing

    Depositor

    Rahul Kohli

    Article

    Berrios et al Nat Chem Biol. 2021 Dec;17(12):1262-1270

    Insert

    Lenti-evoA1-seBEC
    

    Use

    Lentiviral

    Tags

    flag tag

    Expression

    Mutation

    UGI-E11K

    Promoter

    EFS

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. CDK4mut(K35M)-3XFlag-pBabe

    Plasmid

    #73559

    Purpose

    vector encoding a fusion protein between a CDK4 mutant (K35M) and 3 Flag tags

    Depositor

    Gerardo Ferbeyre

    Article

    Acevedo et al Cancer Res. 2016 Jun 1;76(11):3252-64. d

    Insert

    CDK4mut(K35M)
    

    Use

    Retroviral

    Tags

    3X-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    March 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. cycLuc_SuMMVs

    Plasmid

    #119210

    Purpose

    Circularly permutated firefly luciferase with SuMMVs cleavage site (Luciferase reporter for detection of proteolytic activity)

    Depositor

    Roman Jerala

    Article

    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

    Insert

    cyclic luciferase with SuMMVp cleavage site
    

    Use

    Luciferase

    Tags

    AU1 tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pMRX-IP/SECFP-mDFCP1

    Plasmid

    #58765

    Purpose

    Expresses SECFP-mDFCP1 in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Koyama-Honda et al Autophagy. 2013 Oct;9(10):1491-9. doi: 1

    Insert

    ZFYVE1 zinc finger, FYVE domain containing 1 (Zfyve1 Mouse)
    

    Use

    Retroviral

    Tags

    SECFP

    Expression

    Mutation

    Promoter

    Available since

    Jan. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

25. pKD280

    Plasmid

    #125071

    Purpose

    Expression of SO_4387 under Ptac

    Depositor

    Jeffrey Tabor

    Article

    Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

    Inserts

    SO_4387 (SO_4387 )
    lacI (lacI )
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    S286L- Please see depositor comments below

    Promoter

    Available since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. Lenti-sgKmt2d-sg3

    Plasmid

    #192167

    Purpose

    Lenti-sgKmt2d-sg3

    Depositor

    Sidi Chen

    Article

    Wang et al Cancer Discov. 2020 Dec;10(12):1912-1933

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    NA

    Promoter

    Available since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. mEmerald-PDHA1-N-10

    Plasmid

    #54225

    Purpose

    Localization: Mitochondria, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Article

    Planchon et al Nat Methods. 2011 May;8(5):417-23. doi:

    Insert

    PDHA1 (PDHA1 Human)
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 16, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

28. pHR- flag-Lag16-Intb2

    Plasmid

    #205203

    Purpose

    This vector encodes of the synCAM (Intb2) and GFP nanobody LAG16

    Depositor

    Wendell Lim

    Article

    Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

    Insert

    synCAM Intb2 and Lag16 anti-GFP nanobody
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pGMC00014

    Plasmid

    #172526

    Purpose

    non targeting sgRNA

    Depositor

    Raj Chari

    Article

    Ruf et al Cancer Immunol Res. 2021 Jun 30. pii: 23

    Insert

    Non-targeting guide
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. mCherry-E2

    Plasmid

    #175242

    Purpose

    Bacterial expression and purification, E2 enzyme (UBC9) for SUMOylation

    Depositor

    Michael Rosen

    Article

    Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

    Insert

    UBE2I (UBE2I Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge