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pBbB11c-GFP

Plasmid

#126126

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

GFPuv

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23150

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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pBbB10c-GFP

Plasmid

#126125

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

GFPuv

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23116

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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pBbB9c-GFP

Plasmid

#126124

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

GFPuv

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23113

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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pBbA15c-RFP

Plasmid

#126108

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23100

Available Since

June 17, 2019

Availability

Academic Institutions and Nonprofits only

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pBbA14c-RFP

Plasmid

#126099

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23119

Available Since

June 14, 2019

Availability

Academic Institutions and Nonprofits only

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pWS930

Plasmid

#123054

Purpose

Beta-estradiol inducible DNA binding domain

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

Z3E [3a]

Expression

Yeast

Available Since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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HM110 prp16-2

Plasmid

#111277

Purpose

prp16-2 in pSE358(Trp)

Depositor

Christine Guthrie

Article

Madhani et al Genetics. 1994 Jul;137(3):677-87.

Insert

PRP16

Expression

Yeast

Mutation

prp-2 (D575N)

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pUPD2-ATPAMTS (12)

Plasmid

#101696

Purpose

ATPAMTS in domestication vector

Depositor

Elena Caro Bernat

Article

Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

Insert

CCAT-[MTS from S. cerevisiae ATP synthase subunit alpha (YBL099W)]-AATG

Expression

Yeast

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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pUPD2-GLRX2MTS (12)

Plasmid

#101697

Purpose

GLRX2MTS in domestication vector

Depositor

Elena Caro Bernat

Article

Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

Insert

CCAT-[MTS from S. cerevisiae Glutaredoxin-2 (YDR513W)]-AATG

Expression

Yeast

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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pBP-BBa_J23100

Plasmid

#74097

Purpose

BBa_J23100 promoter (iGEM collection)

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

Bba_J23100 constitutive promoter (Sigma70)

Use

Synthetic Biology

Expression

Bacterial

Promoter

None

Available Since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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pAK22

Plasmid

#101741

Purpose

encoding two copies of T. thermophilus SecG for expression of SecYEG complex

Depositor

Tomoya Tsukazaki

Article

Sugano et al PLoS One. 2017 Aug 18;12(8):e0183434. do

Insert

Thermus thermophilus HB8 SecG x 2

Tags

NONE

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pAM-PAT-ProCDKA;1 GW

Plasmid

#79750

Purpose

binary DEST vector for plant expression

Depositor

Nico Dissmeyer, Arp Schnittger

Article

Nowack et al Nat Genet. 2006 Jan;38(1):63-7. Epub 200

Type

Empty backbone

Tags

none

Expression

Plant

Promoter

ProCDKA;1

Available Since

Aug. 21, 2017

Availability

Academic Institutions and Nonprofits only

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Ppms028(JBEI-11601)

Plasmid

#87958

Purpose

jStack yeast assembly into plant vectors, Level 1 Intermediary Vector, p15A origin

Depositor

Dominique Loque

Article

Shih et al Nat Commun. 2016 Oct 26;7:13215. doi: 10

Type

Empty backbone

Use

Synthetic Biology

Available Since

May 3, 2017

Availability

Academic Institutions and Nonprofits only

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pDGE63

Plasmid

#79445

Purpose

recipient plasmid [single nuclease]; p35S:Cas9, BASTA

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Insert

ccdB (ccdB(letD) )

Use

CRISPR

Expression

Plant

Mutation

BsaI site eliminated

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pDGE144

Plasmid

#79448

Purpose

recipient plasmid [multiplexing]; p35S:Cas9, FAST, BASTA

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Insert

ccdB (ccdB(letD) )

Use

CRISPR

Expression

Plant

Mutation

BsaI site eliminated

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pDGE148

Plasmid

#79450

Purpose

recipient plasmid [multiplexing]; pDD45:Cas9, FAST, BASTA

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Insert

ccdB (ccdB(letD) )

Use

CRISPR

Expression

Plant

Mutation

BsaI site eliminated

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pDGE151

Plasmid

#79451

Purpose

recipient plasmid [multiplexing]; pICU2:Cas9, FAST, BASTA

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Insert

ccdB (ccdB(letD) )

Use

CRISPR

Expression

Plant

Mutation

BsaI site eliminated

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pDGE160

Plasmid

#79452

Purpose

recipient plasmid [multiplexing]; p35S:Cas9, FAST, nptII

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Insert

ccdB (ccdB(letD) )

Use

CRISPR

Expression

Plant

Mutation

BsaI site eliminated

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pDGE164

Plasmid

#79454

Purpose

recipient plasmid [multiplexing]; pDD45:Cas9, FAST, nptII

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Insert

ccdB (ccdB(letD) )

Use

CRISPR

Expression

Plant

Mutation

BsaI site eliminated

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pDGE165

Plasmid

#79455

Purpose

recipient plasmid [multiplexing]; pICU2:Cas9, FAST, nptII

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Insert

ccdB (ccdB(letD) )

Use

CRISPR

Expression

Plant

Mutation

BsaI site eliminated

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pDGE166

Plasmid

#79456

Purpose

recipient plasmid [multiplexing]; p35S:Cas9, FAST, Hygro

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Insert

ccdB (ccdB(letD) )

Use

CRISPR

Expression

Plant

Mutation

BsaI site eliminated

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pDGE169

Plasmid

#79459

Purpose

recipient plasmid [multiplexing]; pICU2:Cas9, FAST, Hygro

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Insert

ccdB (ccdB(letD) )

Use

CRISPR

Expression

Plant

Mutation

BsaI site eliminated

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pDGE2

Plasmid

#79441

Purpose

recipient plasmid [multiplexing]; p35S:Cas9, BASTA

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Insert

ccdB (ccdB(letD) )

Use

CRISPR

Expression

Plant

Mutation

BsaI site eliminated

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pBP-TL6

Plasmid

#72990

Purpose

RBS_M3

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

M3 RBS

Use

Synthetic Biology

Mutation

CAT gene - C435G (nucleotide) - silent mutagenesi…

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only
pBP-TL7

Plasmid

#72991

Purpose

RBS_M5

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

M5 RBS

Use

Synthetic Biology

Mutation

CAT gene - C435G (nucleotide) - silent mutagenesi…

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only
pBP-TL9

Plasmid

#72993

Purpose

RBS_L2

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

L2 RBS

Use

Synthetic Biology

Mutation

CAT gene - C435G (nucleotide) - silent mutagenesi…

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pBP-TL10

Plasmid

#72994

Purpose

RBS_L3

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

L3 RBS

Use

Synthetic Biology

Mutation

CAT gene - C435G (nucleotide) - silent mutagenesi…

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only
pBP-TL12

Plasmid

#72996

Purpose

RBS_L5

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

L5 RBS

Use

Synthetic Biology

Mutation

CAT gene - C435G (nucleotide) - silent mutagenesi…

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only
pBP-BBa_B0012

Plasmid

#72997

Purpose

BBa_B0012 iGEM terminator (5' TCGA/ 3' TGTT fusion)

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

Bba_B0012 terminator

Use

Synthetic Biology

Mutation

CAT gene - C435G (nucleotide) - silent mutagenesi…

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pBP-BBa_B0015

Plasmid

#72998

Purpose

BBa_B0015 iGEM terminator (5' TCGA/ 3' TGTT fusion)

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

BBa_B0015 terminator

Use

Synthetic Biology

Mutation

CAT gene - C435G (nucleotide) - silent mutagenesi…

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge