We narrowed to 10,084 results for: Coli
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Plasmid#184970PurposeExpress -Eco1 RT in yeastDepositorInsertIntegrating inducible cassette: Eco1 RT
TagsSV40NLSExpressionYeastMutationhuman codon optimized RTPromoterGal1-10Available SinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLS.543
Plasmid#184960PurposeTest effect of a1/a2 length on editing murF using retron recombineeringDepositorInsertEco1 RT and recombineering ncRNA, murF G1359A, a1/a2 length: 12
ExpressionBacterialMutationmurF donor G1359APromoterT7/lacAvailable SinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLS.545
Plasmid#184962PurposeTest effect of a1/a2 length on editing priB using retron recombineeringDepositorInsertEco1 RT and recombineering ncRNA, priB G1069A, a1/a2 length: 12
ExpressionBacterialMutationpriB donor G1069APromoterT7/lacAvailable SinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKP1693
Plasmid#167595PurposeExpress FepA with His6 tag between residues 393 and 394 of the mature amino acid sequence . Please note that mutation numbers are not based on start codon of insert.DepositorInsertFepA
ExpressionBacterialMutationHis6 tag inserted after residue 393 in the mature…Available SinceJuly 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKB235
Plasmid#170024PurposeEncodes LacI-controlled expression of surface-displayed CCL20 and PvirK-mNeonGreen reporterDepositorArticleInsertsUseSynthetic BiologyTagsLpp, OmpA, and TetherExpressionBacterialPromoterPtac and PvirKAvailable SinceJuly 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMRBad-C-wtBlc
Plasmid#168257PurposeC-fragment. This plasmid can be co-transformed with either Plasmid 168259: pET11a-N-wtBlc or Plasmid 168472: pET11a-N-DiB2 to obtain wtBlc‐split or DiB2‐split protein, correspondinglyDepositorInsertC-fragment of wtBlc
ExpressionBacterialPromoteraraBADAvailable SinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBEL2082
Plasmid#155162PurposeExpresses (Strep-MlaF_Δ[248-269])-(6xHis-TEV-MlaB)DepositorInsert(MlaFΔ[248-269])-(6xHis-TEV-MlaB)
ExpressionBacterialMutationdeleted amino acids 248-269 in MlaFAvailable SinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBEL1514
Plasmid#155147PurposeExpresses MlaFEDCB operon with MlaF C-terminus deleted [1-246], His-tag on MlaDDepositorInsertmlaF(1-246)-mlaE-[6xHis-2xQH-TEV-mlaD]-mlaCB operon
PromoteraraBADAvailable SinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBEL1965
Plasmid#155152PurposeExpresses MlaF(1-246)-(6 aa linker)-GCN4DepositorInsertmlaF(1-246)-GCN4_Dimerization_Domain
Mutationdeleted amino acids 247-269 in MlaF; added dimeri…PromoteraraBADAvailable SinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBEL1966
Plasmid#155153PurposeExpresses MlaF(1-246)-(10 aa linker)-GCN4DepositorInsertmlaF(1-246)-GCN4_Dimerization_Domain
Mutationdeleted amino acids 247-269 in MlaF; added dimeri…PromoteraraBADAvailable SinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBEL1967
Plasmid#155154PurposeExpresses MlaF(1-246)-(14 aa linker)-GCN4DepositorInsertmlaF(1-246)-GCN4_Dimerization_Domain
Mutationdeleted amino acids 247-269 in MlaF; added dimeri…PromoteraraBADAvailable SinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIIS18-BufAI-2
Plasmid#128651PurposeEntry vector useful for golden gate or other seamless cloning technique based on Type IIS restriction enzymesDepositorTypeEmpty backboneUseUnspecifiedAvailable SinceAug. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIIS18-BufAI-1
Plasmid#128650PurposeEntry vector useful for golden gate or other seamless cloning technique based on Type IIS restriction enzymesDepositorTypeEmpty backboneUseUnspecifiedAvailable SinceAug. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPB-DEST-R5R4-pNeo
Plasmid#141058PurposeMutisite Gateway mediated vector constructionDepositorInsertNeomycin resistance gene
ExpressionMammalianAvailable SinceJuly 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHCKan-yibDp-cimA3.7
Plasmid#134595PurposeLow Phosphate inducible mutant citramalate synthaseDepositorInsertcimA3.7
ExpressionBacterialMutationI47V, E114V, H126Q, T204A, L238SPromoteryibDpAvailable SinceMay 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTKhc-yibDp-GFP-β20cp6
Plasmid#127060PurposeLow phosphate inducible circular permutation of GFP, which is a substrate of the lon proteaseDepositorInsertGFPcp6-beta20
TagsHis tagExpressionBacterialPromoteryibDAvailable SinceMay 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJM108
Plasmid#134160PurposeRetroviral plasmid expressing GFP - Nde1DepositorInsertNde1_KMLL E. coli codon optimized (CRISPR/Cas9 hardened) (NDE1 Human)
UseRetroviralTagsGFPExpressionMammalianMutationKMLL, E. coli codon optimized, CRISPR/Cas9 harden…Available SinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJM107
Plasmid#134159PurposeRetroviral plasmid expressing GFP - Nde1DepositorInsertNde1_SSSC E. coli codon optimized (CRISPR/Cas9 hardened) (NDE1 Human)
UseRetroviralTagsGFPExpressionMammalianMutationSSSC, E. coli codon optimized, CRISPR/Cas9 harden…Available SinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLY151
Plasmid#130947PurposepspFΔHTH::λN22plus gene controlled by promoter PrhaBDepositorInsertsrhaS
pspFΔHTH::λN22plus
UseSynthetic BiologyTagspspFΔHTH::λN22plusExpressionBacterialMutationThe HTH domain of the pspF gene was deleted (297-…PromoterPcon and PrhaBAvailable SinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNIC28-BSA4-S15
Plasmid#127821PurposeProduction of recombinant human ribosomal protein S15DepositorAvailable SinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only