We narrowed to 8,039 results for: Dos;

1. pAAV-syn-FLEX-synaptoPAC-minWPRE

   Plasmid

   #153100

   Purpose

   Cre-conditional expression of synaptoPAC, a fusion of synaptophysin, mScarlet, and bPAC in neurons

   Depositor

   Dietmar Schmitz

   Article

   Oldani et al J Neurochem. 2021 Feb;156(3):324-336. do

   Inserts

   bPAC(WT)
   Synaptophysin (Syp Rat)
   

   Use

   AAV

   Tags

   His tag, mScarlet, and myc tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDG335

   Plasmid

   #100899

   Purpose

   SpCas9n (D10A Nickase mutant) with a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of DSBs with no off-target cuts.

   Depositor

   Paul Thomas

   Article

   Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do

   Inserts

   humanized CRISPR associated protein 9 Nickase
   U6-gRNA scaffold 1
   U6-gRNA scaffold 2
   

   Use

   CRISPR and Mouse Targeting

   Tags

   HA

   Expression

   Mammalian

   Mutation

   D10A mutant converts to Nickase

   Promoter

   CBh and U6

   Available since

   Dec. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET21-pelB-LaG-21

   Plasmid

   #172749

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-21; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-21
   

   Use

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET21-pelB-LaG-37

   Plasmid

   #172756

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-37; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-37
   

   Use

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. CMV_Npu-BE3.9max C-terminal

   Plasmid

   #137172

   Purpose

   Expresses the C-terminal split-intein fragment of BE3.9max from the CMV promoter

   Depositor

   David Liu

   Article

   Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

   Insert

   Npu-BE3.9max_C-terminal
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. IL2 pAcGP67a

   Plasmid

   #41806

   Depositor

   Chris Garcia

   Article

   Levin et al Nature. 2012 Mar 25;484(7395):529-33. do

   Insert

   IL2 (IL2 Human)
   

   Use

   Tags

   6x His

   Expression

   Insect

   Mutation

   Promoter

   Polyhedron

   Available since

   Jan. 3, 2013

   Availability

   Academic Institutions and Nonprofits only

7. CMV_Npu-ABEmax C-terminal

   Plasmid

   #137174

   Purpose

   Expresses the C-terminal split-intein fragment of ABEmax from the CMV promoter

   Depositor

   David Liu

   Article

   Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

   Insert

   Npu-ABEmax_C-terminal
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAGIG_V5-PPP6C

   Plasmid

   #92013

   Purpose

   Expresses V5-PPP6C, includes IRES-Emerald

   Depositor

   Joshua Mendell

   Article

   Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

   Insert

   PPP6C (PPP6C Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pH4R-mCherry-N1

   Plasmid

   #84328

   Purpose

   Histamine-4 receptor tagged with mCherry

   Depositor

   Dorus Gadella

   Article

   van Unen et al Mol Pharmacol. 2016 Sep;90(3):162-76. do

   Insert

   H4R-mCherry (HRH4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSLIK S6K (E389-deltaCT) neo

    Plasmid

    #58516

    Purpose

    Lentiviral expression vector for an inducible HA-tagged rat S6K1-E389-deltaCT

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

    Insert

    Rps6kb1 (Rps6kb1 Rat)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    T389E, stop codon at amino acid 402

    Promoter

    Available since

    May 5, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pET21-pelB-LaG-41

    Plasmid

    #172757

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-41; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-41
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-hCjeCas9-NLS(SV40)-3xFLAG (KAC579)

    Plasmid

    #133789

    Purpose

    CAG promoter expression plasmid for human codon optimized CjeCas9 nuclease with C-terminal NLS (SV40)

    Depositor

    Joseph Bondy-Denomy, Benjamin Kleinstiver

    Article

    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

    Insert

    human codon optimized CjeCas9
    

    Use

    Tags

    NLS(SV40)-3xFLAG

    Expression

    Mammalian

    Mutation

    n/a

    Promoter

    CAG

    Available since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pETM33_TNKS_TANK-peptide-bind

    Plasmid

    #178489

    Purpose

    Bacterial expression of human domain with His-tag and GST tag

    Depositor

    Ylva Ivarsson

    Article

    Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

    Insert

    TNKS_TANK-peptide-bind (TNKS Synthetic, Human)
    

    Use

    Tags

    His-GST

    Expression

    Bacterial

    Mutation

    Promoter

    T7/LacO

    Available since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pETM33_MAD2L1_HORMA

    Plasmid

    #178480

    Purpose

    Bacterial expression of human domain with His-tag and GST tag

    Depositor

    Ylva Ivarsson

    Article

    Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

    Insert

    MAD2L1_HORMA (MAD2L1 Synthetic, Human)
    

    Use

    Tags

    His-GST

    Expression

    Bacterial

    Mutation

    Promoter

    T7/LacO

    Available since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. gRNA_DNMT3a-T1

    Plasmid

    #41821

    Purpose

    Expresses a guide RNA (gRNA) to target DNMT3a (T1 target sequence) for genome engineering

    Depositor

    George Church

    Article

    Mali et al Science. 2013 Feb 15;339(6121):823-6. do

    Insert

    gRNA_DNMT3a-T1 (DNMT3A Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSMP-Bmi1_1

    Plasmid

    #36352

    Depositor

    George Daley

    Article

    Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

    Insert

    Bmi1 (BMI1 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti-CaMKIIa-eArch 3.0-EYFP

    Plasmid

    #35514

    Purpose

    Lentiviral expression of CaMKIIa-driven Arch 3.0 for optical inhibition.

    Depositor

    Karl Deisseroth

    Article

    Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

    Insert

    eArch3.0-EYFP
    

    Use

    Lentiviral

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Enhanced trafficking signal and ER export signal …

    Promoter

    CaMKIIa

    Available since

    May 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. iRFP713-N2-Frankenbody-Flag

    Plasmid

    #175291

    Purpose

    Visualize endogeneous loci coupled with ZF probes harboring 10xFLAG tag

    Depositor

    Tim Stasevich

    Article

    Liu et al Genes Cells. 2021 Nov;26(11):905-926. do

    Insert

    anti-FLAG frankenbody fused with iRFP713
    

    Use

    Tags

    iRFP713

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 3, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pET21-pelB-LaG-11

    Plasmid

    #172762

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-11; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-11
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET21-pelB-LaM-1

    Plasmid

    #172767

    Purpose

    Bacterial expression of anti-mCherry nanobody LaM-1, with pelB leader and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaM-1
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge