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pRSETb-tVYN-TmΔ

Plasmid

#149694

Purpose

High-copy plasmid to express the tVYN-TmΔ luminescent biosensor for in vitro purification

Depositor

Ming Hammond

Article

Dippel et al ACS Chem Biol. 2020 Mar 25. doi: 10.1021

Insert

VenusΔC12-TmYcgRΔ-NLucΔ4

Expression

Bacterial

Promoter

T7

Available Since

Aug. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pCW-12S-E1A-HA-puro

Plasmid

#212671

Purpose

Express adenovirus E1A

Depositor

Yumi Konagaya, Tobias Meyer

Article

Konagaya et al Nature. 2024 Jul;631(8020):424-431. doi:

Insert

12S-E1A

Use

Lentiviral

Tags

HA

Promoter

TRE

Available Since

Aug. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pTet-ctl

Plasmid

#136693

Purpose

Control for pTet-Gli2shR

Depositor

LuZhe Sun

Article

Zhu et al Lab Invest. 2018 Nov;98(11):1384-1396. d

Type

Empty backbone

Use

Lentiviral

Available Since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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pTGFP-T7-Hha10T

Plasmid

#65427

Purpose

Parent vector for constructing non-replicating lesion-bearing plasmid

Depositor

Yinsheng Wang

Article

You et al Nat Protoc. 2015 Sep;10(9):1389-406. doi

Type

Empty backbone

Expression

Mammalian

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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pBD-0016

Plasmid

#12345

Depositor

Axel Brunger

Article

DeLaBarre et al Mol Cell. 2006 May 19. 22(4):451-62.

Insert

VCP R313K (Vcp Mouse)

Tags

His6

Expression

Bacterial

Mutation

R313K, I206V*(see below)

Available Since

Aug. 24, 2006

Availability

Academic Institutions and Nonprofits only
pAG506-mito-frFAST

Plasmid

#160057

Purpose

Expresses mito-frFAST in mammalian cells

Depositor

Arnaud Gautier

Article

Li et al Angew Chem Int Ed Engl. 2020 Jun 22. doi

Insert

Mito-frFAST

Expression

Mammalian

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pMiR-HPV16-E7

Plasmid

#53696

Purpose

Luciferase reporter assay for microRNA binding on HPV16 E7

Depositor

Edward Chan

Article

Jung et al Mol Cancer. 2014 Apr 8;13(1):80. doi: 10

Insert

HPV16-E7

Use

Luciferase

Available Since

June 17, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-PB1-AG

Plasmid

#178861

Purpose

Mammalian expression of PB1-AzamiGreen

Depositor

Shinya Tsukiji

Article

Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

Insert

PB1-AzamiGreen

Expression

Mammalian

Promoter

CMV

Available Since

March 3, 2022

Availability

Academic Institutions and Nonprofits only

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mCherry-PLEKHG4

Plasmid

#129618

Purpose

RhoGEF

Depositor

Dorus Gadella

Article

Reinhard et al Small GTPases. 2019 Aug 30:1-15. doi: 10

Insert

PLEKHG4

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pJSK619

Plasmid

#205134

Purpose

Expression of SnxA-GFP in Dictyostelium

Depositor

Jason King

Article

Vines et al J Cell Biol. 2023 Sep 4;222(9):e20220907

Insert

SnxA

Use

Dictyostelium expression

Tags

EGFP

Available Since

Oct. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pSADdeltaG-GFP-rtTA

Plasmid

#32648

Depositor

Edward Callaway

Article

Osakada et al Neuron. 2011 Aug 25;71(4):617-31.

Inserts

GFP
rtTA

Use

Rabies virus

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 3, 2011

Availability

Academic Institutions and Nonprofits only
LRT2B-AdGO

Plasmid

#136910

Purpose

Lentivirus expressing AdGO sgRNA

Depositor

Lukas Dow

Article

Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

Insert

sgAdGO

Use

Lentiviral

Mutation

WT

Promoter

hU6

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pET30b-7D-SrtA

Plasmid

#105600

Purpose

expresses 7D sortase A variant in E.coli

Depositor

Stephanie Dougan

Article

Jeong et al PLoS One. 2017 Dec 4;12(12):e0189068. do

Insert

S.aureus Sortase A, 7D mutant

Tags

His tag

Expression

Bacterial

Promoter

T7

Available Since

Jan. 22, 2018

Availability

Academic Institutions and Nonprofits only

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UAS-7xHalo7::CAAX

Plasmid

#87647

Purpose

For creating drosophila transgenics expressing UAS-7xHalo7::CAAX

Depositor

Gregory Jefferis

Article

Sutcliffe et al Genetics. 2017 Apr;205(4):1399-1408. doi

Insert

7xHalo7::CAAX

Expression

Insect

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pCabp5-DsRed

Plasmid

#11157

Depositor

Connie Cepko

Article

Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

Insert

Cabp5 promoter (Cabp5 Mouse)

Tags

DsRed2

Expression

Mammalian

Available Since

April 13, 2006

Availability

Academic Institutions and Nonprofits only

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pSADdeltaG-GFP-ER(T2)CreER(T2)

Plasmid

#32649

Depositor

Edward Callaway

Article

Osakada et al Neuron. 2011 Aug 25;71(4):617-31.

Inserts

GFP
ER(T2)CreER(T2)

Use

Rabies virus

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 13, 2012

Availability

Academic Institutions and Nonprofits only
pAAV-hSyn-BREP

Plasmid

#172340

Purpose

AAV transfer plasmid for BREP (bioluminescent red protein)

Depositor

Huiwang Ai

Article

Tian et al Nat Commun. 2022 Jul 8;13(1):3967. doi:

Insert

BREP

Use

AAV

Promoter

hSyn promoter

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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mCherry-PLEKHG2

Plasmid

#129617

Purpose

RhoGEF

Depositor

Dorus Gadella

Article

Reinhard et al Small GTPases. 2019 Aug 30:1-15. doi: 10

Insert

PLEKHG2

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pJL005

Plasmid

#92327

Purpose

Express FtsZ tagged with mEos3.2 in E coli

Depositor

Jie Xiao

Article

Lyu et al Biopolymers. 2016 Oct;105(10):725-34. do

Insert

FtsZ

Tags

mEos3.2

Expression

Bacterial

Promoter

T5-lac

Available Since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pAG499-FRB-N-frFAST

Plasmid

#160059

Purpose

Expresses FRB-N-frFAST in mammalian cells

Depositor

Arnaud Gautier

Article

Li et al Angew Chem Int Ed Engl. 2020 Jun 22. doi

Insert

FRB-N-frFAST

Expression

Mammalian

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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