We narrowed to 5,233 results for: puc

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pILFPmTagBFP2FrFAST

Plasmid

#223839

Purpose

Expressing mTagBFP2-FrFAST under the control of the TEF1 promtoer in Saccharomyces cerevisiae

Depositor

Claudia Vickers

Article

Evans et al ACS Synth Biol. 2025 Sep 19;14(9):3804-3

Insert

pTEF1>mTagBFP2>FrFAST

Expression

Yeast

Promoter

TEF1

Available Since

June 23, 2025

Availability

Academic Institutions and Nonprofits only

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pDCX_mEmerald_CRISPR_HDR

Plasmid

#239331

Purpose

HDR template to insert mEmerald before the stop codon in exon 7 of the human doublecortin (DCX) locus

Depositor

Torsten Wittmann

Article

Dema et al Curr Biol. 2024 Nov 21:S0960-9822(24)014

Insert

mEmerald and DCX homology arms

Use

CRISPR

Promoter

None

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pwA-ple34

Plasmid

#227337

Purpose

Heterologous production of pleuromutilin in Aspergillus oryzae

Depositor

Jun-ichi Maruyama

Insert

The upstream/inside regions of the wA gene and expression cassettes of the ple3 and ple4 genes

Use

Synthetic Biology

Available Since

Jan. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pNHA13

Plasmid

#223645

Purpose

Expression of mxiE-turboID fusion

Depositor

François-Xavier Campbell-Valois

Article

Haidar-Ahmad et al mSphere. 2024 Nov 21;9(11):e0055324. doi

Inserts

mxiE (mxiE )
turboID

Tags

Myc

Expression

Bacterial

Promoter

rpsM

Available Since

Oct. 28, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pNHA14

Plasmid

#223646

Purpose

Expression of turboID-mxiE fusion

Depositor

François-Xavier Campbell-Valois

Article

Haidar-Ahmad et al mSphere. 2024 Nov 21;9(11):e0055324. doi

Inserts

mxiE (mxiE )
turboID

Tags

Myc

Expression

Bacterial

Promoter

rpsM

Available Since

Oct. 28, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pGGF-bGH polyA-G

Plasmid

#218902

Purpose

golden gate cloning

Depositor

Sven Eyckerman

Article

Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

Insert

bGH polyA

Use

Synthetic Biology

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA371

Plasmid

#215989

Purpose

Fragmid fragment: (N' terminus) attenuate expression

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Start; u(S)ORF_v1.1; Stop; Start; HIV-NES_v1.2; HIV-NES_v1.1

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pPG-GPD-TRPC

Plasmid

#190113

Purpose

Expression vector for fungal (Aspergillus flavus) genes based on Aspergillus parasiticus pyrG gene selection

Depositor

Perng-Kuang Chang

Article

Chang et al Fungal Genet Biol. 2017 Jul;104:29-37. d

Type

Empty backbone

Expression

Yeast

Available Since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pTS1037

Plasmid

#169616

Purpose

Tier-2 vector encoding PmPGK1-driven secreted Nluc (A1-pA::A2-pA::PmPGK1-IgkSS-Nluc-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PPGK-driven expression of secreted Nluc

Expression

Mammalian

Promoter

PPGK

Available Since

Sept. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pTS1035

Plasmid

#169614

Purpose

Tier-2 vector encoding PmPGK1-driven secreted Nluc (PmPGK1-IgkSS-Nluc-pA::A2-pA::A3-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PPGK-driven expression of secreted Nluc

Expression

Mammalian

Promoter

PPGK

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pTS1032

Plasmid

#169612

Purpose

Tier-2 vector encoding PhCMV-driven secreted Nluc (A1-pA::PhCMV-IgkSS-Nluc-pA::A3-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven expression of secreted Nluc

Expression

Mammalian

Promoter

PhCMV

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pTS1031

Plasmid

#169611

Purpose

Tier-2 vector encoding PhCMV-driven secreted Nluc (PhCMV-IgkSS-Nluc-pA::A2-pA::A3-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven expression of secreted Nluc

Expression

Mammalian

Promoter

PhCMV

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pTS1033

Plasmid

#169613

Purpose

Tier-2 vector encoding PhCMV-driven secreted Nluc (A1-pA::A2-pA::PhCMV-IgkSS-Nluc-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven expression of secreted Nluc

Expression

Mammalian

Promoter

PhCMV

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pTS1036

Plasmid

#169615

Purpose

Tier-2 vector encoding PmPGK1-driven secreted Nluc (A1-pA::PmPGK1-IgkSS-Nluc-pA::A3-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PPGK-driven expression of secreted Nluc

Expression

Mammalian

Promoter

PPGK

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-RiboJ-mTFP1-T7

Plasmid

#173225

Purpose

Encodes mTFP fluorescent protein. To be used in cell-free system

Depositor

Jim Haseloff

Article

Guzman-Chavez et al ACS Synth Biol. 2022 Mar 18;11(3):1114-1

Insert

mTFP1

Use

Cell-free system

Expression

Bacterial

Promoter

T7

Available Since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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Rp51a_SUS1 5'SS

Plasmid

#100991

Purpose

Contains model Rp51a with a GGTAtGT->tGTAtGa 5'SS mutant (SUS1 5'SS sequence).

Depositor

Aaron Hoskins

Article

Larson et al Elife. 2017 Aug 22;6. pii: 27592. doi: 1

Insert

RP51A (RPS17A Budding Yeast)

Expression

Bacterial and Yeast

Mutation

GGTAtGT->tGTAtGa 5'SS mutant

Available Since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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ANXA11_Halo_C_allele

Plasmid

#178136

Purpose

Donor vector for endogenous tagging of human ANXA11 at the C-terminus with halotag

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Halotag (ANXA11 Human)

Use

Donor vector

Available Since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

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PANK2_Halo_C_allele

Plasmid

#178145

Purpose

Donor vector for endogenous tagging of human PANK2 at the C-terminus with halotag

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Halotag (PANK2 Human)

Use

Donor vector

Available Since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

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FBXO7_Halo_C_allele

Plasmid

#178158

Purpose

Donor vector for endogenous tagging of human FBXO7 at the C-terminus with halotag

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Halotag (FBXO7 Human)

Use

Donor vector

Available Since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pZBED9-donor

Plasmid

#176344

Purpose

CRISPR donor plasmid to create GFP fusion proteins

Depositor

Barbara Stranger, Kevin White

Insert

ZBED9 (ZBED9 Human)

Use

CRISPR

Tags

GFP

Available Since

Nov. 12, 2021

Availability

Academic Institutions and Nonprofits only

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