Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 15,577 results for: pET

Showing: 8881 - 8920 of 15577 results

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pAc5.1B-lambdaN-HA-DmPAN3-F658EL622EL665EW707AG711E-dsRNAres_Y

Plasmid

#148082

Purpose

Insect Expression of DmPAN3-F658EL622EL665EW707AG711E-dsRNAres

Depositor

Elisa Izaurralde

Article

Jonas et al Nat Struct Mol Biol. 2014 Jul;21(7):599-

Insert

DmPAN3-F658EL622EL665EW707AG711E-dsRNAres (CG11486 Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmPAN3-FLLWGYHRtoEEEAEAAA-dsRNAres_Y

Plasmid

#148083

Purpose

Insect Expression of DmPAN3-FLLWGYHRtoEEEAEAAA-dsRNAres

Depositor

Elisa Izaurralde

Article

Jonas et al Nat Struct Mol Biol. 2014 Jul;21(7):599-

Insert

DmPAN3-FLLWGYHRtoEEEAEAAA-dsRNAres (CG11486 Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmPAN3-FLLYHRtoEEEAAA-dsRNAres_Y

Plasmid

#148084

Purpose

Insect Expression of DmPAN3-FLLYHRtoEEEAAA-dsRNAres

Depositor

Elisa Izaurralde

Article

Jonas et al Nat Struct Mol Biol. 2014 Jul;21(7):599-

Insert

DmPAN3-FLLYHRtoEEEAAA-dsRNAres (CG11486 Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-lambdaN-HA-DmPAN3-G711E-dsRNAres_Y

Plasmid

#148085

Purpose

Insect Expression of DmPAN3-G711E-dsRNAres

Depositor

Elisa Izaurralde

Article

Jonas et al Nat Struct Mol Biol. 2014 Jul;21(7):599-

Insert

DmPAN3-G711E-dsRNAres (CG11486 Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-lambdaN-HA-DmPAN3-L766EV768E-dsRNAres_Y

Plasmid

#148086

Purpose

Insect Expression of DmPAN3-L766EV768E-dsRNAres

Depositor

Elisa Izaurralde

Article

Jonas et al Nat Struct Mol Biol. 2014 Jul;21(7):599-

Insert

DmPAN3-L766EV768E-dsRNAres (CG11486 Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-lambdaN-HA-DmPAN3-W707AG711EV765R-dsRNAres_Y

Plasmid

#148088

Purpose

Insect Expression of DmPAN3-W707AG711EV765R-dsRNAres

Depositor

Elisa Izaurralde

Article

Jonas et al Nat Struct Mol Biol. 2014 Jul;21(7):599-

Insert

DmPAN3-W707AG711EV765R-dsRNAres (CG11486 Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-lambdaN-HA-DmPAN3-W707E-dsRNAres_Y

Plasmid

#148089

Purpose

Insect Expression of DmPAN3-W707E-dsRNAres

Depositor

Elisa Izaurralde

Article

Jonas et al Nat Struct Mol Biol. 2014 Jul;21(7):599-

Insert

DmPAN3-W707E-dsRNAres (CG11486 Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-EGFP-DmPAN2-L766EV758E-dsRNAres_Y

Plasmid

#148047

Purpose

Insect Expression of DmPAN3-L766EV758E-dsRNAres

Depositor

Elisa Izaurralde

Article

Jonas et al Nat Struct Mol Biol. 2014 Jul;21(7):599-

Insert

DmPAN3-L766EV758E-dsRNAres (CG11486 Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-EGFP-DmPAN3-dsRNAres_Y

Plasmid

#148050

Purpose

Insect Expression of DmPAN3-dsRNAres

Depositor

Elisa Izaurralde

Article

Jonas et al Nat Struct Mol Biol. 2014 Jul;21(7):599-

Insert

DmPAN3-dsRNAres (CG11486 Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-EGFP-DmPAN3-E679LG683V-dsRNAres_Y

Plasmid

#148051

Purpose

Insect Expression of DmPAN3-E679LG683V-dsRNAres

Depositor

Elisa Izaurralde

Article

Jonas et al Nat Struct Mol Biol. 2014 Jul;21(7):599-

Insert

DmPAN3-E679LG683V-dsRNAres (CG11486 Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-EGFP-DmPAN3-F658EL622EL665EW707AG711E-dsRNAres_Y

Plasmid

#148052

Purpose

Insect Expression of DmPAN3-F658EL622EL665EW707AG711E-dsRNAres

Depositor

Elisa Izaurralde

Article

Jonas et al Nat Struct Mol Biol. 2014 Jul;21(7):599-

Insert

DmPAN3-F658EL622EL665EW707AG711E-dsRNAres (CG11486 Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-EGFP-DmPAN3-FLLWGYHRtoEEEAEAAA-dsRNAres_Y

Plasmid

#148053

Purpose

Insect Expression of DmPAN3-FLLWGYHRtoEEEAEAAA-dsRNAres

Depositor

Elisa Izaurralde

Article

Jonas et al Nat Struct Mol Biol. 2014 Jul;21(7):599-

Insert

DmPAN3-FLLWGYHRtoEEEAEAAA-dsRNAres (CG11486 Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-EGFP-DmPAN3-FLLYHRtoEEEAAA-dsRNAres_Y

Plasmid

#148054

Purpose

Insect Expression of DmPAN3-FLLYHRtoEEEAAA-dsRNAres

Depositor

Elisa Izaurralde

Article

Jonas et al Nat Struct Mol Biol. 2014 Jul;21(7):599-

Insert

DmPAN3-FLLYHRtoEEEAAA-dsRNAres (CG11486 Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-EGFP-DmPAN3-G711E-dsRNAres_Y

Plasmid

#148055

Purpose

Insect Expression of DmPAN3-G711E-dsRNAres

Depositor

Elisa Izaurralde

Article

Jonas et al Nat Struct Mol Biol. 2014 Jul;21(7):599-

Insert

DmPAN3-G711E-dsRNAres (CG11486 Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-EGFP-DmPAN3-W707AG711E-dsRNAres_Y

Plasmid

#148056

Purpose

Insect Expression of DmPAN3-W707AG711E-dsRNAres

Depositor

Elisa Izaurralde

Article

Jonas et al Nat Struct Mol Biol. 2014 Jul;21(7):599-

Insert

DmPAN3-W707AG711E-dsRNAres (CG11486 Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-EGFP-DmPAN3-W707AG711EV765R-dsRNAres_Y

Plasmid

#148057

Purpose

Insect Expression of DmPAN3-W707AG711EV765R-dsRNAres

Depositor

Elisa Izaurralde

Article

Jonas et al Nat Struct Mol Biol. 2014 Jul;21(7):599-

Insert

DmPAN3-W707AG711EV765R-dsRNAres (CG11486 Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-lambdaN-HA-DmPAN2_377-1241_Y

Plasmid

#148061

Purpose

Insect Expression of DmPAN2_377-1241

Depositor

Elisa Izaurralde

Article

Jonas et al Nat Struct Mol Biol. 2014 Jul;21(7):599-

Insert

DmPAN2_377-1241 (PAN2 Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-EGFP-DmPAN2_377-1241_Y

Plasmid

#148030

Purpose

Insect Expression of DmPAN2_377-1241

Depositor

Elisa Izaurralde

Article

Jonas et al Nat Struct Mol Biol. 2014 Jul;21(7):599-

Insert

DmPAN2_377-1241 (PAN2 Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-EGFP-DmPAN2-dsRNAres_Y

Plasmid

#148041

Purpose

Insect Expression of DmPAN2-dsRNAres

Depositor

Elisa Izaurralde

Article

Jonas et al Nat Struct Mol Biol. 2014 Jul;21(7):599-

Insert

DmPAN2-dsRNAres (PAN2 Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-lambdaN-HA-DmCup_1-417-DmeIF4G_686-1666_T

Plasmid

#147584

Purpose

Insect Expression of DmCup_1-417-DmeIF4G_686-1666

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

DmCup_1-417-DmeIF4G_686-1666 (cup Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-EGFP-Dm4E-T_1-510-Y10AL15A-V5His6_T

Plasmid

#147600

Purpose

Insect Expression of Dm4E-T_1-510-Y10AL15A

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

Dm4E-T_1-510-Y10AL15A (4E-T Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_rSERT_C109A_Y107C

Plasmid

#190174

Purpose

For expression of a SERT mutant in mammalian cells and measuring accessibility in the SERT extracellular pathway

Depositor

Gary Rudnick

Article

Jacobs et al J Biol Chem. 2007 Oct 5;282(40):29441-7.

Insert

SLC6A4 Serotonin Transporter (Slc6a4 Rat)

Tags

FLAG, His6, and c-myc

Expression

Mammalian

Mutation

changed Cys109 to alanine, changed tyr107 to cyst…

Promoter

CMV

Available Since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pUC19-sg-1

Plasmid

#190607

Purpose

Expresses a gRNA for base editing the EGFP Kozak sequence of pWPT-/mEGFP-1T-IRES-mCherry

Depositor

Alessandro Quattrone

Article

Ambrosini et al Nucleic Acids Res. 2022 Oct 14;50(18):10

Insert

gRNA sequence

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pUC19-sgNCF1

Plasmid

#190193

Purpose

Expresses a gRNA for base editing the Kozak sequence of the human NCF1 gene

Depositor

Alessandro Quattrone

Article

Ambrosini et al Nucleic Acids Res. 2022 Oct 14;50(18):10

Insert

gRNA sequence

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-EGFP-Dm4E-T-L36AL39A_T

Plasmid

#147604

Purpose

Insect Expression of Dm4E-T-L36AL39A

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

Dm4E-T-L36AL39A (4E-T Fly)

Expression

Insect

Mutation

Two non silent mutations (H628R and N659S) compar…

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-EGFP-DmCup-Y342AL347A_O

Plasmid

#147103

Purpose

Insect Expression of DmCup-Y342AL347A

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

DmCup-Y342AL347A (cup Fly)

Expression

Insect

Mutation

Y342A, L347A, I350L, P418S, K1045N

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-lambdaN-HA-DmPAN3-dsRNAres_Y

Plasmid

#148080

Purpose

Insect Expression of DmPAN3-dsRNAres

Depositor

Elisa Izaurralde

Article

Jonas et al Nat Struct Mol Biol. 2014 Jul;21(7):599-

Insert

DmPAN3-dsRNAres (CG11486 Fly)

Expression

Insect

Available Since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-lambdaN-HA-Dm4E-T_1-510-Y10AL15A-V5His6_D

Plasmid

#146152

Purpose

Insect Expression of Dm4E-T_1-510-Y10AL15A

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

Dm4E-T_1-510-Y10AL15A (4E-T Fly)

Expression

Insect

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCI-Flag-NEDD4L∆WW4

Plasmid

#166710

Purpose

expresses NEDD4L with inactivating mutation in fourth WW domain in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

Insert

NEDD4L W576A

Tags

FLAG

Expression

Mammalian

Mutation

changed tryptophan 576 to alanine

Promoter

CMV promoter

Available Since

July 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-PP-NEDD4L-WW1(193-226)

Plasmid

#166701

Purpose

expresses NEDD4L WW1 in bacterial cells

Depositor

Wesley Sundquist

Article

Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

Insert

NEDD4L WW1 domain

Tags

GST, HRV 3C site, and Thrombin site

Expression

Bacterial

Promoter

tac promoter

Available Since

July 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-PP-NEDD4L-WW2(385-418)

Plasmid

#166702

Purpose

expresses NEDD4L WW2 in bacterial cells

Depositor

Wesley Sundquist

Article

Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

Insert

NEDD4L WW2 domain

Tags

GST, HRV 3C site, and Thrombin site

Expression

Bacterial

Promoter

tac promoter

Available Since

July 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-PP-NEDD4L-WW3(497-530)

Plasmid

#166703

Purpose

expresses NEDD4L WW3 in bacterial cells

Depositor

Wesley Sundquist

Article

Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

Insert

NEDD4L WW3 domain

Tags

GST, HRV 3C site, and Thrombin site

Expression

Bacterial

Promoter

tac promoter

Available Since

July 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-PP-NEDD4L-WW4(548-581)

Plasmid

#166704

Purpose

expresses NEDD4L WW4 in bacterial cells

Depositor

Wesley Sundquist

Article

Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

Insert

NEDD4L WW4 domain

Tags

GST, HRV 3C site, and Thrombin site

Expression

Bacterial

Promoter

tac promoter

Available Since

July 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCI-Flag-NEDD4L∆WW1

Plasmid

#166707

Purpose

expresses NEDD4L with inactivating mutation in first WW domain in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

Insert

NEDD4L W221A

Tags

FLAG

Expression

Mammalian

Mutation

changed tryptophan 221 to alanine

Promoter

CMV promoter

Available Since

July 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV.U6gLacZ.EFS-NS.H2B-RFP

Plasmid

#170363

Purpose

Negative control plasmid used for Crispr/cas9 based disruption. Expressing nuclear RFP.

Depositor

Johan Jakobsson

Article

Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

Insert

H2B-RFP

Use

Lentiviral

Promoter

EFS-NS

Available Since

June 27, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
CMV-10xQb oligo-library based

Plasmid

#158201

Purpose

Cassette of 10 different binding sites with high affinity to QB phage coat protein, as extracted from the oligo pool experiment.

Depositor

Roee Amit

Article

Katz et al Nat Commun. 2021 Mar 11;12(1):1576. doi:

Insert

10 binding sites of QB based on oligo library experiment

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX6P-1 gnu

Plasmid

#112999

Purpose

For protein expression and purification of full-length Drosophila gnu

Depositor

Terry Orr-Weaver

Article

Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

Insert

full length gnu (gnu Fly)

Expression

Bacterial

Available Since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pAd388-BPTF-sh28

Plasmid

#83276

Purpose

shRNA targeting BPTF

Depositor

Joe Landry

Article

Mayes et al Oncotarget. 2017 May 12;8(38):64344-6435

Insert

shRNA to BPTF

Use

Adenoviral

Promoter

pUC ori

Available Since

Nov. 8, 2016

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pcDNA3.1-FLAG-SBP-STAG2 (Y1142fs)

Plasmid

#73969

Purpose

STAG2 cDNA (CCDS43990) Y1142fs

Depositor

Todd Waldman

Article

Kim et al PLoS Genet. 2016 Feb 12;12(2):e1005865.

Insert

STAG2 (STAG2 Human)

Tags

FLAG-SBP

Expression

Mammalian

Mutation

Y1142fs

Available Since

March 14, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-FLAG-SBP-STAG2 (I885fs)

Plasmid

#73966

Purpose

STAG2 cDNA (CCDS43990) I885fs

Depositor

Todd Waldman

Article

Kim et al PLoS Genet. 2016 Feb 12;12(2):e1005865.

Insert

STAG2 (STAG2 Human)

Tags

FLAG-SBP

Expression

Mammalian

Mutation

I885fs

Available Since

March 7, 2016

Availability

Academic Institutions and Nonprofits only

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