We narrowed to 40,052 results for: lat

1. pENTR2b-ITGB1(YYFF)

   Plasmid

   #215451

   Purpose

   Gateway entry vector with integrin beta1 NPxY mutant (YYFF)

   Depositor

   Johanna Ivaska

   Article

   Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

   Insert

   ITGB1 (ITGB1 Human)
   

   Use

   Gateway entry vector

   Mutation

   Mutations in the NPxY sites Y783F, Y795F; Silent …

   Promoter

   none

   Available Since

   May 29, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pENTR2b-ITGB1(WT)-mRuby2

   Plasmid

   #215446

   Purpose

   Gateway entry vector with integrin beta1 tagged with mRuby2

   Depositor

   Johanna Ivaska

   Article

   Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

   Insert

   ITGB1 (ITGB1 Human)
   

   Use

   Gateway entry vector

   Tags

   mRuby2

   Mutation

   Silent mutations added to disrupt shRNA binding a…

   Promoter

   none

   Available Since

   May 29, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pDGB3_alpha1 nptII-DISTC02-CBS-FRT-Rep-RepA-tNos-DISTC19-CBS-miniDFR-FLP-tNos-PROXC02-pNos-CUP2-Gal4AD-tNos-SF (GB4697)

   Plasmid

   #225440

   Purpose

   Module for copper and flippase-regulated expression of BeYDV Rep/RepA, including the TU for copper-regulated FLP, the TU for constitutive expression of CUP2-GAL4, and the nptII cassette.

   Depositor

   Diego Orzaez

   Article

   Garcia-Perez et al Plant Biotechnol J. 2025 Jan;23(1):141-1

   Insert

   nptII-DISTC02-CBS-FRT:Rep/RepA:tNos-DISTC19-CBS:miniDFR:FLP:tNos-PROXC02-pNos:CUP2:Gal4AD:tNos-SF
   

   Use

   Synthetic Biology

   Mutation

   BsaI and BsmBI sites removed

   Available Since

   Oct. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDual

   Plasmid

   #207883

   Purpose

   Expresses thermosensitive variants of the homing endonucleases I-OnuI and I-GpeMI, each in a different open reading frame. Both were made thermosensitive by a fusion with the L212P VMA1 intein.

   Depositor

   David Edgell

   Article

   Foo et al Nucleic Acids Res. 2024 Feb 28;52(4):206

   Inserts

   Thermosensitive fusion of I-OnuI and the L212P VMA1 intein.
   Thermosensitive fusion of I-GpeMI and the L212P VMA1 intein.
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   Leucine 212 changed to proline, leucine 434 in th…

   Available Since

   Aug. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMRXIP-GFP-0K9Q

   Plasmid

   #221743

   Purpose

   Stable expression of GFP-0K9Q in mammalian cells to monitor membrane charge

   Depositor

   Noboru Mizushima

   Article

   Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

   Insert

   C -terminal region of KRAS (KRAS Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   deleted amino acids 1-170, mutated K to Q

   Available Since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMRXIP-GFP-1K8Q

   Plasmid

   #221742

   Purpose

   Stable expression of GFP-1K8Q in mammalian cells to monitor membrane charge

   Depositor

   Noboru Mizushima

   Article

   Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

   Insert

   C -terminal region of KRAS (KRAS Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   deleted amino acids 1-170, mutated K to Q

   Available Since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMRXIP-GFP-3K6Q

   Plasmid

   #221741

   Purpose

   Stable expression of GFP-3K6Q in mammalian cells to monitor membrane charge

   Depositor

   Noboru Mizushima

   Article

   Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

   Insert

   C -terminal region of KRAS (KRAS Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   deleted amino acids 1-170, mutated K to Q

   Available Since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMRXIP-GFP-5K4Q

   Plasmid

   #221740

   Purpose

   Stable expression of GFP-5K4Q in mammalian cells to monitor membrane charge

   Depositor

   Noboru Mizushima

   Article

   Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

   Insert

   C -terminal region of KRAS (KRAS Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   deleted amino acids 1-170, mutated K to Q

   Available Since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMXS-IRES-BLAST flag-CAD M1601E

   Plasmid

   #169828

   Purpose

   Expresses C-terminal flag-tagged CAD with mutation at reported dimerization interface of the DHOase domain

   Depositor

   Richard Possemato

   Article

   Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

   Insert

   CAD (CAD Human)
   

   Use

   Retroviral

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   M1601E; TCCC -> AGTC silent mutations at nt527…

   Available Since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-TNT4-TadA8e-SpRY N aa2-713-InteinN-miR122 TS

    Plasmid

    #209787

    Purpose

    Expresses TadA8e and SpRY cas9N by the specific TNT4 promoter and incorporation of the miR122 target sequences (miR122TS) into the 3’ untranslated region (3’ UTR)

    Depositor

    Yuxuan Guo

    Article

    Yang et al Circulation. 2024 May 28;149(22):1778-17

    Insert

    TNT4, TadA8e, SpRY N, inteinN, miR122 TS
    

    Use

    AAV

    Promoter

    TNT4

    Available Since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3(+)-UFBP1 F196A V198A-MYC

    Plasmid

    #204556

    Purpose

    Expresses FLAG-tagged UFBP1 F196A V198A in mammalian cells

    Depositor

    Masaaki Komatsu

    Article

    Ishimura et al Nat Commun. 2022 Dec 21;13(1):7857. doi:

    Insert

    UFBP1 (DDRGK1 Human)
    

    Expression

    Mammalian

    Mutation

    F196A V198A

    Available Since

    Aug. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAsh-MCL-LC3B

    Plasmid

    #204573

    Purpose

    Expresses Ash-tagged LC3B in mammalian cells

    Depositor

    Masaaki Komatsu

    Article

    Ishimura et al Nat Commun. 2022 Dec 21;13(1):7857. doi:

    Insert

    LC3B (MAP1LC3B Human)
    

    Expression

    Mammalian

    Mutation

    delta121-125aa

    Available Since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHis-3xFLAG-CMV14-CYB5R3 K214R

    Plasmid

    #204552

    Purpose

    Expresses 3xFLAG-tagged CYB5R3 K214R in mammalian cells

    Depositor

    Masaaki Komatsu

    Article

    Ishimura et al Nat Commun. 2022 Dec 21;13(1):7857. doi:

    Insert

    CYB5R3 (CYB5R3 Human)
    

    Expression

    Mammalian

    Mutation

    K214R

    Available Since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pENeCFPCNAL2 pc922

    Plasmid

    #167563

    Purpose

    For construction of CFP-tagged human PCNA, the GFP coding sequence in the pENeGFPCNAL2 vector was replaced by the ECFP coding sequence from the pECFP-C1 vector (Clontech Laboratories, Inc., CA).

    Depositor

    Cristina Cardoso

    Article

    Zhang et al Nucleic Acids Res. 2017 Apr 25. doi: 10.

    Inserts

    Proliferating Cell Nuclear Antigen (PCNA Human)
    CFP
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSUMO mouse METAP1 1-79

    Plasmid

    #197397

    Purpose

    Expresses SUMO-tagged mouse METAP1, residues 1-79

    Depositor

    David Giedroc

    Article

    Weiss et al Cell. 2022 Jun 9;185(12):2148-2163.e27.

    Insert

    METAP1 1-79 (Metap1 Mouse)
    

    Tags

    SUMO

    Expression

    Bacterial

    Available Since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSUMO mouse METAP1 1-59

    Plasmid

    #197396

    Purpose

    Expresses SUMO-tagged mouse METAP1, residues 1-59

    Depositor

    David Giedroc

    Article

    Weiss et al Cell. 2022 Jun 9;185(12):2148-2163.e27.

    Insert

    METAP1 1-59 (Metap1 Mouse)
    

    Tags

    SUMO

    Expression

    Bacterial

    Available Since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. Coexpressed Free deadFPPS-NES

    Plasmid

    #182492

    Purpose

    Yeast integrative plasmid for expressing ERG20 (GAL10 promoter) and fusion protein deadFPPS-AcNES1 (GAL7 promoter). deadFPPS is ERG20(K197G-K254A), an inactive mutant of ERG20.

    Depositor

    Claudia Vickers

    Article

    Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.

    Inserts

    FPPS
    deadFPPS-NES
    

    Use

    Synthetic Biology; Metabolic engineering

    Expression

    Yeast

    Promoter

    GAL10 and GAL7

    Available Since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAGGS-chGrb2/eDHFR(69K6)-iRFP713

    Plasmid

    #178854

    Purpose

    Mammalian expression of Grb2-eDHFR(69K6) chimera fused to iRFP713

    Depositor

    Shinya Tsukiji

    Article

    Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

    Insert

    Grb2(1-59)-eDHFR(69K6)-Grb2(152-216)-iRFP713 (GRB2 Human)
    

    Expression

    Mammalian

    Mutation

    Grb2: Y160E

    Promoter

    CAG

    Available Since

    July 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPBpuro-miRFP670-eDHFR(69K6)-G-alpha-s

    Plasmid

    #172105

    Purpose

    Mammalian expression of Gαs fused to miRFP670-eDHFR(69K6)

    Depositor

    Shinya Tsukiji

    Article

    Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

    Insert

    miRFP670-eDHFR(69K6)-Gαs (GNAS Human)
    

    Expression

    Mammalian

    Mutation

    Gαs: C3S

    Promoter

    CAG

    Available Since

    July 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pmirGlo-RBM8A-3'UTRAluY-Sz mut 2598-4066

    Plasmid

    #171135

    Purpose

    reporter

    Depositor

    Emanuela Felley-Bosco

    Article

    Abukar et al Cells. 2021 Dec 15;10(12). pii: cells101

    Insert

    RBM8A 3'UTR (RBM8A Human)
    

    Expression

    Mammalian

    Mutation

    A3302G, A3356G, A3373G, A3389G,A3451G, T3729C, A3…

    Available Since

    Dec. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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