Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

Filter By

We narrowed to 9,954 results for: rras

Showing: 4441 - 4460 of 9954 results

  1. TRE3GV-SpdCas9-Dnmt3A/3L-WT

    Plasmid
    #222502
    Purpose
    Expresses TRE3GV promoter driven human codon optimized SpdCas9 directly fused to WT Dnmt3A/3L and hPGK promoter driven mCherry
    Depositor
    Isaac Hilton
    Article
    Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021
    Inserts
    S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L engineered fusion
    mCherry
    Use
    Lentiviral
    Tags
    FLAG Tag
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9
    Promoter
    TRE3GV and hPGK
    Available since
    Feb. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    222502-plasmid-map-sequence-id-441221

    Enlarge

  2. EFS-SpdCas9-Dnmt3A/3L-V1

    Plasmid
    #222498
    Purpose
    Expresses EFS promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant V1 (R887E) followed by P2A-mCherry
    Depositor
    Isaac Hilton
    Article
    Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021
    Insert
    S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant V1 (R887E) engineered fusion
    Use
    Lentiviral
    Tags
    FLAG Tag
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9; R887E in Dnmt3A
    Promoter
    EFS
    Available since
    Feb. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    222498-plasmid-map-sequence-id-441135

    Enlarge

  3. EFS-SpdCas9-Dnmt3A/3L-V2

    Plasmid
    #222499
    Purpose
    Expresses EFS promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant V2 (E814G) followed by P2A-mCherry
    Depositor
    Isaac Hilton
    Article
    Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021
    Insert
    S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant V2 (E814G) engineered fusion
    Use
    Lentiviral
    Tags
    FLAG Tag
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9; E814G in Dnmt3A
    Promoter
    EFS
    Available since
    Feb. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    222499-plasmid-map-sequence-id-441136

    Enlarge

  4. EFS-SpdCas9-Dnmt3A/3L-V3

    Plasmid
    #222500
    Purpose
    Expresses EFS promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant V3 (R887E and E814G) followed by P2A-mCherry
    Depositor
    Isaac Hilton
    Article
    Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021
    Insert
    S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant V3 (R887E and E814G) engineered fusion
    Use
    Lentiviral
    Tags
    FLAG Tag
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9; R887E and E814…
    Promoter
    EFS
    Available since
    Feb. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    222500-plasmid-map-sequence-id-441137

    Enlarge

  5. TRE3GV-SpdCas9-Dnmt3A/3L-V1

    Plasmid
    #222503
    Purpose
    Expresses TRE3GV promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant V1 (R887E) and hPGK promoter driven mCherry
    Depositor
    Isaac Hilton
    Article
    Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021
    Inserts
    S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant V1 (R887E) engineered fusion
    mCherry
    Use
    Lentiviral
    Tags
    FLAG Tag
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9; R887E in Dnmt3A
    Promoter
    TRE3GV and hPGK
    Available since
    Feb. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    222503-plasmid-map-sequence-id-441222

    Enlarge

  6. TRE3GV-SpdCas9-Dnmt3A/3L-V2

    Plasmid
    #222505
    Purpose
    Expresses TRE3GV promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant V2 (E814G) and hPGK promoter driven mCherry
    Depositor
    Isaac Hilton
    Article
    Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021
    Inserts
    S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant V2 (E814G) engineered fusion
    mCherry
    Use
    Lentiviral
    Tags
    FLAG Tag
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9; E814G in Dnmt3A
    Promoter
    TRE3GV and hPGK
    Available since
    Feb. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    222505-plasmid-map-sequence-id-441223

    Enlarge

  7. TRE3GV-SpdCas9-Dnmt3A/3L-V3

    Plasmid
    #222506
    Purpose
    Expresses TRE3GV promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant V3 (R887E and E814G) and hPGK promoter driven mCherry
    Depositor
    Isaac Hilton
    Article
    Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021
    Inserts
    S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant V3 (R887E and E814G) engineered fusion
    mCherry
    Use
    Lentiviral
    Tags
    FLAG Tag
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9; R887E and E814…
    Promoter
    TRE3GV and hPGK
    Available since
    Feb. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    222506-plasmid-map-sequence-id-441224

    Enlarge

  8. TRE3GV-SpdCas9-Dnmt3A/3L-catΔ

    Plasmid
    #222508
    Purpose
    Expresses TRE3GV promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant catΔ (C706A and R832E) and hPGK promoter driven mCherry
    Depositor
    Isaac Hilton
    Article
    Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021
    Inserts
    S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant catΔ (C706A and R832E) engineered fusion
    mCherry
    Use
    Lentiviral
    Tags
    FLAG Tag
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9; C706A and R832…
    Promoter
    TRE3GV and hPGK
    Available since
    Feb. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    222508-plasmid-map-sequence-id-441225

    Enlarge

  9. pT7-EGFP-C1-HsNot1iso1-del1097-1110-IKGRQtoDSYAA-shRNAres_W

    Plasmid
    #147897
    Purpose
    Mammalian Expression of HsNot1iso1-del1097-1110-IKGRQtoDSYAA-shRNAres
    Depositor
    Elisa Izaurralde
    Article
    Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:
    Insert
    HsNot1iso1-del1097-1110-IKGRQtoDSYAA-shRNAres (CNOT1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    10 silent mutations and two non silent N605S, K75…
    Promoter
    Available since
    Nov. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    147897-plasmid-map-sequence-id-290344

    Enlarge

  12. pT7-EGFP-C1-HsNot1iso1_1-1605-del1079-1110-RNFtoAAA-shRNAres_W

    Plasmid
    #147886
    Purpose
    Mammalian Expression of HsNot1iso1_1-1605-del1097-1110-RNFtoAAA-shRNAres
    Depositor
    Elisa Izaurralde
    Article
    Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:
    Insert
    HsNot1iso1_1-1605-del1097-1110-RNFtoAAA-shRNAres (CNOT1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    1 silent mutation compared to the sequence of iso…
    Promoter
    Available since
    Nov. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    147886-plasmid-map-sequence-id-290333

    Enlarge

  13. pT7-EGFP-C1-HsNot1iso1_1-1605-del1097-1110-RNFIKGRQtoAAADSYAA-shRNAres_W

    Plasmid
    #147887
    Purpose
    Mammalian Expression of HsNot1iso1_1-1605-del1097-1110-RNFIKGRQtoAAADSYAA-shRNAres
    Depositor
    Elisa Izaurralde
    Article
    Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:
    Insert
    HsNot1iso1_1-1605-del1097-1110-RNFIKGRQtoAAADSYAA-shRNAres (CNOT1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    1 silent mutation compared to the sequence of iso…
    Promoter
    Available since
    Nov. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    147887-plasmid-map-sequence-id-290334

    Enlarge

  14. CbetaS-pA

    Plasmid
    #32104
    Depositor
    Michael Cole, Yardena Samuels
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    cmv
    Available since
    Sept. 16, 2011
    Availability
    Academic Institutions and Nonprofits only

  15. pSm-Nuclease

    Plasmid
    #132431
    Purpose
    Expresses Serratia marcescens nuclease in E. coli using T7 promoter
    Depositor
    Mark Arbing
    Insert
    Serratia marcescens nuclease (codon optimized)
    Use
    Tags
    None
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Oct. 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    132431-plasmid-map-sequence-id-258770

    Enlarge

  16. pK2-K3

    Plasmid
    #218221
    Purpose
    Fuses Guides 1+2 to guides 3+4 in an of AtU6-promter driven sgRNAs
    Depositor
    Daniel Voytas
    Insert
    linker sequence
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    218221-plasmid-map-sequence-id-431988

    Enlarge

  17. pK4-K5

    Plasmid
    #218222
    Purpose
    Fuses Guides 3+4 to guides 6+6 in an of AtU6-promter driven sgRNAs
    Depositor
    Daniel Voytas
    Insert
    linker sequence
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    218222-plasmid-map-sequence-id-431983

    Enlarge

  18. pK6-K7

    Plasmid
    #218223
    Purpose
    Fuses Guides 5+6 to guides 7+8 in an of AtU6-promter driven sgRNAs
    Depositor
    Daniel Voytas
    Insert
    linker sequence
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    218223-plasmid-map-sequence-id-431979

    Enlarge

  19. pENTR ARRET

    Plasmid
    #111384
    Purpose
    ARRET with approximately 130 telomere hexanucleotide repeats of TTAGGG and TTGGGG variant sequences
    Depositor
    Klaus Holzmann
    Insert
    ARRET
    Use
    Gateway entry cloning
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    111384-plasmid-map-sequence-id-209223

    Enlarge

  20. 2Q3F

    Plasmid
    #51356
    Depositor
    Cheryl Arrowsmith
    Insert
    2Q3F (RRAGD Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Truncation: See Comments
    Promoter
    T7
    Available since
    Feb. 12, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 4441 - 4460 of 9954 results