We narrowed to 6,523 results for: sas

1. pRO16Sh5

   Plasmid

   #180076

   Purpose

   Expression of ShCAST under control of Plac with pRO1600 origin.

   Depositor

   Christopher Reisch

   Article

   Trujillo Rodriguez et al ACS Synth Biol. 2023 Jun 27. doi: 10.102

   Insert

   ShTnsB, ShTnsC, ShTniQ, ShCas12k
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   pLac

   Available Since

   July 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTXB1-Tn5

   Plasmid

   #60240

   Purpose

   IPTG-inducible expression of Transposon Tn5 fused to Mxe Intein and Chitin-binding domain.

   Depositor

   Rickard Sandberg

   Article

   Picelli et al Genome Res. 2014 Jul 30. pii: gr.177881.

   Insert

   tnpA from pBam1
   

   Tags

   Mxe intein - Chitin-binding domain

   Expression

   Bacterial

   Mutation

   E54K, L372P

   Promoter

   T7 promoter

   Available Since

   Nov. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. VR122 pETv2 10His-pG-Tn5 (E54K, L372P)

   Plasmid

   #198467

   Purpose

   E. coli protein expression plasmid encoding hyperactive Tn5 transposase fused to protein G, in DH5α cloning strain.

   Depositor

   Viviana Risca

   Article

   Soroczynski et al bioRxiv 2024.07.11.602973

   Insert

   10His-pG-Tn5 (E54K, L372P)
   

   Tags

   10His-pG

   Expression

   Bacterial

   Mutation

   E54K, L372P

   Promoter

   Consensus φ10 T7 Promoter

   Available Since

   April 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. phL1A-pcDNA3

   Plasmid

   #12307

   Depositor

   Vance Lemmon

   Article

   Hlavin et al Genomics. 1991 Oct . 11(2):416-23.

   Insert

   L1CAM (L1CAM Human)
   

   Expression

   Mammalian

   Available Since

   Oct. 7, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET21-HIS-Tol2

   Plasmid

   #112486

   Purpose

   functional recombinant Tol2 protein from E.coli

   Depositor

   Stephen Ekker

   Article

   Ni et al Mob DNA. 2016 Mar 31;7:6. doi: 10.1186/s

   Insert

   Tol2 Transposase (tpase Synthetic)
   

   Tags

   HIS/6x

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSBtet-Hygro-GADD34_Δ1-240/P2A/K3L

   Plasmid

   #196136

   Purpose

   SB vector for the mammalian expression of GADD34-K3L

   Depositor

   Jamie Cate

   Article

   Aleksashin et al bioRxiv. 2023 Feb 10:2023.02.09.527910.

   Insert

   GADD34 Δ1-240 - P2A - K3L (PPP1R15A Human, vaccinia virus)
   

   Tags

   P2A

   Expression

   Mammalian

   Available Since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pQCasTns(Ptr)-array 8

   Plasmid

   #175580

   Purpose

   Expression of CRISPR-associated transposases

   Depositor

   Sheng Yang

   Article

   Yang et al Nucleic Acids Res. 2021 Sep 3. pii: 6363

   Insert

   PtrTniQ, PtrCas5/8, PtrCas7, PtrCas6, PtrTnsABC, CRISPR(8 CRISPR array)
   

   Expression

   Bacterial

   Mutation

   NO

   Available Since

   Jan. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMC-J6-dCas9-10XGP41-J9

   Plasmid

   #202005

   Purpose

   MoClo level 0 dCas9-10XGP41 coding sequence module

   Depositor

   Michael Smanski

   Article

   Casas-Mollano et al Nucleic Acids Research, Volume 51, Issue

   Insert

   dCas9-10XGP41
   

   Use

   Synthetic Biology; Moclo cloning vector

   Tags

   GS linkers

   Available Since

   July 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pQCasTns(Ptr)-IS186

   Plasmid

   #175581

   Purpose

   Expression of CRISPR-associated transposases

   Depositor

   Sheng Yang

   Article

   Yang et al Nucleic Acids Res. 2021 Sep 3. pii: 6363

   Insert

   PtrTniQ, PtrCas5/8, PtrCas7, PtrCas6, PtrTnsABC, CRISPR(IS186)
   

   Expression

   Bacterial

   Mutation

   NO

   Available Since

   Jan. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTetQCas-8+IS186

    Plasmid

    #170636

    Purpose

    Expression of CRISPR-associated transposases

    Depositor

    Sheng Yang

    Article

    Zhang et al CRISPR J. 2021 Jun;4(3):350-359. doi: 10

    Insert

    VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(8+IS186 array)
    

    Expression

    Bacterial

    Available Since

    Nov. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMC-J6-dCas9-24XGP41-J9

    Plasmid

    #202006

    Purpose

    MoClo level 0 dCas9-24XGP41 coding sequence module

    Depositor

    Michael Smanski

    Article

    Casas-Mollano et al Nucleic Acids Research, Volume 51, Issue

    Insert

    dCas9-24XGP41
    

    Use

    Synthetic Biology; Moclo cloning vector

    Tags

    GS linkers

    Available Since

    July 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBLlSh2

    Plasmid

    #170981

    Purpose

    Expression of ShCAST under control of lacI Plac promoter/repressor pair.

    Depositor

    Christopher Reisch

    Article

    Trujillo Rodriguez et al ACS Synth Biol. 2023 Jun 27. doi: 10.102

    Inserts

    lacI
    ShTnsB, ShTnsC, ShTniQ, ShCas12k
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    pLlac01

    Available Since

    July 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET3CTc3A(1-135)

    Plasmid

    #63138

    Purpose

    Expression of human Tc3(1–135) bipartite domain tagged with 6xHis

    Depositor

    Titia Sixma

    Article

    Watkins et al Nucleic Acids Res. 2004 Aug 10;32(14):43

    Insert

    Tc3A
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    1-135 aa bipartite domain

    Promoter

    T7

    Available Since

    April 17, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pTBY1-T4gp61/41 fusion

    Plasmid

    #228210

    Purpose

    Expression in BL21(DE3) of T4 primase and helicase fusion with 24 amino acid linker

    Depositor

    Stephen Benkovic

    Article

    Manosas et al Nat Chem Biol. 2009 Dec;5(12):904-12. do

    Insert

    61/41
    

    Tags

    Sce VMA intein-chitin binding domain

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. VR124 pETv2 10His-pG-Tn5 (E54K, L372P)

    Plasmid

    #198468

    Purpose

    E. coli protein expression plasmid encoding hyperactive Tn5 transposase fused to protein G, in T7 Express lysY/Iq protein expression strain.

    Depositor

    Viviana Risca

    Article

    Soroczynski et al bioRxiv 2024.07.11.602973

    Insert

    10His-pG-Tn5 (E54K, L372P)
    

    Tags

    10His-pG

    Expression

    Bacterial

    Mutation

    E54K, L372P

    Promoter

    Consensus φ10 T7 Promoter

    Available Since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pN7CAST

    Plasmid

    #190661

    Purpose

    Expresses polycistronic N7 CAST where Cas12k-T2A-TnsC and TniQ-T2A-TnsB are separated by an EMCV IRES

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    human codon optimized N7Cas12k, N7TnsC, N7TniQ, N7TnsB
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHelper_ShCAST_sgRNA

    Plasmid

    #127921

    Purpose

    Expresses ShCAST and an empty sgRNA scaffold. New targets can be added using Golden Gate assembly (LguI sites).

    Depositor

    Feng Zhang

    Article

    Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

    Insert

    ShTnsB, ShTnsC, ShTniQ, ShCas12k, sgRNA scaffold for guide cloning (LguI sites)
    

    Available Since

    July 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTnsABC

    Plasmid

    #160031

    Purpose

    Expresses V. cholerae TnsABC

    Depositor

    Sheng Yang

    Article

    Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

    Insert

    VchTnsA, VchTnsB, VchTnsC
    

    Use

    CRISPR; Transposon

    Expression

    Bacterial

    Mutation

    no

    Available Since

    Nov. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pN7HELIX

    Plasmid

    #190662

    Purpose

    Expresses polycistronic N7 HELIX where Cas12k-T2A-TnsC and TniQ-T2A-nAniI_TnsB are separated by an EMCV IRES

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    human codon optimized N7Cas12k, N7TnsC, N7TniQ, nAniI_N7TnsB
    

    Expression

    Mammalian

    Mutation

    nAniI = K227M, F80K, L232K

    Promoter

    CMV

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDonor14-AcHELIX-KanR (CJT96)

    Plasmid

    #181794

    Purpose

    pDonor for AcHELIX containing 14bp of spacing between the I-AniI site and LE/RE using ShCAST flanking sequence and encoding a kanamycin resistance gene as cargo

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    KanR, R6K origin of replication
    

    Expression

    Bacterial

    Available Since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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