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PGK-HALO-CTERM-GFP

Plasmid

#185777

Purpose

Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

Depositor

Alessandra Ianari, William Sellers

Article

Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

Insert

GFP

Use

Lentiviral

Tags

V5, Halo

Mutation

none

Promoter

PGK

Available Since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

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PGK-HALO-NTERM-GFP

Plasmid

#185772

Purpose

Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

Depositor

Alessandra Ianari, William Sellers

Article

Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

Insert

GFP

Use

Lentiviral

Tags

V5, Halo

Mutation

none

Promoter

PGK

Available Since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pET-scFv-T

Plasmid

#67843

Purpose

pET-30-based vector dedicated to efficient scFv expression, which circumvents the problem of in-frame amber codons in scFvs.

Depositor

Joanna Bereta

Article

Ossysek et al Immunol Lett. 2015 Jul 25. pii: S0165-24

Insert

antibody 1D8 light kappa chain

Tags

His-tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pNH605-PACE-YFP

Plasmid

#172002

Purpose

Fluorescent reporter for Rpn4 activity

Depositor

Johannes Herrmann

Article

Boos et al Nat Cell Biol. 2019 Apr;21(4):442-451. d

Insert

4xPACE-YFP

Use

Vector for genomic integration into the yeast gen…

Expression

Yeast

Promoter

CYC1 core promotor with 4 tandem repeats of Rpn4 …

Available Since

Nov. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pDF0338 pAAV U6-HvsCas7-11 DR Rev-BpiI golden gate backbone dual vector

Plasmid

#172514

Purpose

Encodes HvsCas7-11 DR and golden gate site for spacer clonings in an AAV backbone with U6 promoter

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

Insert

pAAV U6-HvsCas7-11 DR Rev-BpiI golden gate backbone dual vector

Use

AAV

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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eGFP-KCC2

Plasmid

#104075

Purpose

expresses rat KCC2 (b isoform) with eGFP tag at the N-terminus of KCC2

Depositor

Igor Medyna

Article

Pellegrino et al J Physiol. 2011 May 15;589(Pt 10):2475-9

Insert

KCC2 (b isoform) (Slc12a5 Rat)

Tags

EGFP

Expression

Mammalian

Mutation

EcoR1 site in rat KCC2 was removed by silent muta…

Promoter

CMV

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pNH605-CCAAT-YFP

Plasmid

#172132

Purpose

Fluorescent reporter for HAP complex activity

Depositor

Johannes Herrmann

Article

Boos et al Nat Cell Biol. 2019 Apr;21(4):442-451. d

Insert

4xCCAAT-YFP

Use

Vector for genomic integration into the yeast gen…

Expression

Yeast

Promoter

CYC1 core promotor with 4 tandem repeats of the H…

Available Since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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mMaple-OMP25

Plasmid

#141151

Purpose

Photoconvertible fluorescent protein targeted to the outer mitochondrial membrane (OMM) in mammalian cells.

Depositor

Gia Voeltz

Article

Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

OMP25 (Synj2bp Rat)

Tags

mMaple

Expression

Mammalian

Mutation

OMP25 c terminus from addgene plasmid #69598

Promoter

CMV

Available Since

Aug. 31, 2020

Availability

Academic Institutions and Nonprofits only

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SFFV-IKZF3-CTERM-GFP

Plasmid

#185769

Purpose

Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

Depositor

Alessandra Ianari, William Sellers

Article

Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

Insert

GFP

Use

Lentiviral

Tags

V5, IKZF3 minidegron

Mutation

none

Promoter

SFFV

Available Since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

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CD99-CD4d3+4-bio

Plasmid

#73097

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding CD99 with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

CD99 (CD99 Human)

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Promoter

CMV

Available Since

March 28, 2016

Availability

Academic Institutions and Nonprofits only

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PGK-AID-CTERM-GFP

Plasmid

#185778

Purpose

Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

Depositor

Alessandra Ianari, William Sellers

Article

Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

Insert

GFP

Use

Lentiviral

Tags

V5, AID

Mutation

none

Promoter

PGK

Available Since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pBABE HA Chk2 WT

Plasmid

#41901

Depositor

Stephen Elledge

Article

Matsuoka et al Science. 2007 May 25;316(5828):1160-6.

Insert

CHK2 (CHEK2 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Available Since

Aug. 15, 2013

Availability

Academic Institutions and Nonprofits only

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ICAM4-CD4d3+4-bio

Plasmid

#73102

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding ICAM4 with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

ICAM4 (ICAM4 Human)

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Promoter

CMV

Available Since

March 28, 2016

Availability

Academic Institutions and Nonprofits only

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CD55-CD4d3+4-bio

Plasmid

#73116

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding CD55 with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

CD55 (CD55 Human)

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Promoter

CMV

Available Since

March 28, 2016

Availability

Academic Institutions and Nonprofits only

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Lactotransferrin-CD4d3+4-bio

Plasmid

#73124

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding Lactotransferrin with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

Lactotransferrin (LTF Human)

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Promoter

CMV

Available Since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

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SFFV-HALO-NTERM-GFP

Plasmid

#185762

Purpose

Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

Depositor

Alessandra Ianari, William Sellers

Article

Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

Insert

GFP

Use

Lentiviral

Tags

V5, Halo

Mutation

none

Promoter

SFFV

Available Since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

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AchE-CD4d3+4-bio

Plasmid

#73119

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding AchE with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

AchE (ACHE Human)

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Promoter

CMV

Available Since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

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ERMAP-CD4d3+4-bio

Plasmid

#73107

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding ERMAP with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

ERMAP (ERMAP Human)

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Promoter

CMV

Available Since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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pInducer20 Cyclin E1

Plasmid

#109348

Purpose

virus packaged, doxycyline inducible expression of Cyclin E1

Depositor

Jean Cook

Article

Matson et al Elife. 2017 Nov 17;6. doi: 10.7554/eLife

Insert

Cyclin E1 (CCNE1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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Lutheran-CD4d3+4-bio

Plasmid

#73101

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding Lutheran with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

Lutheran (BCAM Human)

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Promoter

CMV

Available Since

March 28, 2016

Availability

Academic Institutions and Nonprofits only

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