We narrowed to 18,681 results for: tec

1. pnCas9-PBE

   Plasmid

   #98164

   Purpose

   Expresses CRISPR base editor in protoplasts

   Depositor

   Caixia Gao

   Article

   Zong et al Nat Biotechnol. 2017 May;35(5):438-440.

   Insert

   NLS-APOBEC1-XTEN-nCas9-UGI-NLS
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Ubi

   Available since

   July 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCfB6633

   Plasmid

   #106162

   Purpose

   EasyCloneYALI system-based yeast gRNA expression vector carrying a nourseothricin-resistance marker, helps to integrate vector pCfB6677 into Yarrowia lipolytica chromosomal location IntE_1, amp resistance

   Depositor

   Irina Borodina

   Article

   Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

   Insert

   unknown
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBJ1

   Plasmid

   #167130

   Purpose

   A gentamycin resistant plasmid for Tn7-mediated chromosomal integration of Photorhabdus luminescens luxCDABE operon with Pfrr promoter

   Depositor

   Akira Mine

   Article

   Matsumoto et al Plant Commun. 2021 Jul 20;3(1):100227. d

   Insert

   luxCDABE
   

   Use

   Luciferase

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Frr promoter

   Available since

   June 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCS2+ GFP-2xESP3I-IRES-mCherry

   Plasmid

   #230996

   Purpose

   Protein stability reporter construct for transient expression in mammalian cells. Stability of N-terminal GFP-fusion protein can be assessed by flow cytometry by normalizing to mCherry expression.

   Depositor

   Michael Rape

   Article

   Manford et al Cell. 2020 Oct 1;183(1):46-61.e21. doi:

   Type

   Empty backbone

   Use

   Tags

   AcGFP1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pN7CAST

   Plasmid

   #190661

   Purpose

   Expresses polycistronic N7 CAST where Cas12k-T2A-TnsC and TniQ-T2A-TnsB are separated by an EMCV IRES

   Depositor

   Benjamin Kleinstiver

   Article

   Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   human codon optimized N7Cas12k, N7TnsC, N7TniQ, N7TnsB
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. Tad-CBE(TCG2)

   Plasmid

   #235068

   Purpose

   Expresses Tad-CBE(TCG2) in mammalian cells.

   Depositor

   Weixin Tang

   Article

   Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

   Insert

   Tad-CBE(TCG2)-SpCas9 D10A
   

   Use

   CRISPR

   Tags

   2xUGI, bpNLS and bpNLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. Tad-CBE(CCA3)

   Plasmid

   #235070

   Purpose

   Expresses Tad-CBE(CCA3) in mammalian cells.

   Depositor

   Weixin Tang

   Article

   Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

   Insert

   Tad-CBE(CCA3)-SpCas9 D10A
   

   Use

   CRISPR

   Tags

   2xUGI, bpNLS and bpNLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. Tad-CBE(CCT2)

   Plasmid

   #235071

   Purpose

   Expresses Tad-CBE(CCT2) in mammalian cells.

   Depositor

   Weixin Tang

   Article

   Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

   Insert

   Tad-CBE(CCT2)-SpCas9 D10A
   

   Use

   CRISPR

   Tags

   2xUGI, bpNLS and bpNLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. Tad-CBE(TCC2)

   Plasmid

   #235067

   Purpose

   Expresses Tad-CBE(TCC2) in mammalian cells.

   Depositor

   Weixin Tang

   Article

   Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

   Insert

   Tad-CBE(TCC2)-SpCas9 D10A
   

   Use

   CRISPR

   Tags

   2xUGI, bpNLS and bpNLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDonor14-AcHELIX-KanR (CJT96)

    Plasmid

    #181794

    Purpose

    pDonor for AcHELIX containing 14bp of spacing between the I-AniI site and LE/RE using ShCAST flanking sequence and encoding a kanamycin resistance gene as cargo

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    KanR, R6K origin of replication
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pT7-ZoAl-ENT-3xF-ORF

    Plasmid

    #238484

    Purpose

    For production of C-term flag-tagged ZoAl EN-tuned ORF mRNA for mRNA transfection in PRINT

    Depositor

    Kathleen Collins

    Article

    Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

    Insert

    R2 retroelement mRNA
    

    Use

    Ivt template

    Tags

    3x-Flag

    Expression

    Mutation

    R1103A

    Promoter

    Available since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pU3-gRNA

    Plasmid

    #53063

    Purpose

    Transcription of guide RNA (gRNA) in rice cells

    Depositor

    Caixia Gao

    Article

    Shan et al Nat Biotechnol. 2013 Aug;31(8):686-8. do

    Insert

    OsU3 promoter + gRNA scaffold
    

    Use

    CRISPR; Plant expression

    Tags

    Expression

    Mutation

    Promoter

    rice U3

    Available since

    Aug. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. JD631

    Plasmid

    #160399

    Purpose

    Binary vector pGWB14 including Vitis GRF4-GIF1 under 35S promoter/ Plant transformation

    Depositor

    Jorge Dubcovsky

    Article

    Debernardi et al Nat Biotechnol. 2020 Oct 12. pii: 10.103

    Insert

    35S::vitis GRF4-GIF1 chimera
    

    Use

    Binary vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. BPK2660

    Plasmid

    #70709

    Purpose

    Human expression plasmid for SaCas9 sgRNA(84nt in length) (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sa-sgRNA(84)

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pX330 spCas9-mSA

    Plasmid

    #113096

    Purpose

    pX330 vector carrying spCas9-mSA for gene editing in mammalian cell lines

    Depositor

    Janet Rossant

    Article

    Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

    Insert

    CAS9-MSA
    

    Use

    Synthetic Biology

    Tags

    MSA (monomeric streptavidin

    Expression

    Mutation

    none

    Promoter

    Available since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDY1314_RADARS_Luciferase

    Plasmid

    #193191

    Purpose

    Express Gaussia Luciferase conditional upon RNA transcript

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Jiang et al Nat Biotechnol. 2022 Oct 27. pii: 10.103

    Insert

    RADARS Luciferase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHR_Gal4UASpyb-TATA_tBFP_pGK_mCitrine (RL407)

    Plasmid

    #194217

    Purpose

    Lentiviral transducition vector for a GAL4-responsive tagBFP reporter gene

    Depositor

    Hana El-Samad

    Article

    Weinberg et al bioRxiv 2021.04.20.440678

    Insert

    UAS-ybTATA-tagBFP; PGK-mCitrine
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    GAL4 UAS-ybTATA; PGK

    Available since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. ABE-RA4.2

    Plasmid

    #208307

    Purpose

    Expresses ABE-RA4.2 in mammalian cells

    Depositor

    Weixin Tang

    Article

    Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

    Insert

    TadA-RA4.2-SpCas9 D10A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    P48A, R51H, I76F, A106V, D108G, K110R, T111H, D11…

    Promoter

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRN227

    Plasmid

    #127222

    Purpose

    BeYDV replicon with 35S::Cas9, WUS2 and AtSTM, sgRNA targeting PDS

    Depositor

    Daniel Voytas

    Article

    Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

    Insert

    Cas9, WUS2, AtSTM, sgRNA
    

    Use

    Tags

    Expression

    Plant

    Mutation

    codon optimized CDSs

    Promoter

    Available since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. JD639

    Plasmid

    #160398

    Purpose

    Binary vector pGWB14 including miR396-resistant Vitis GRF4-GIF1 under 35S promoter/Plant transformation

    Depositor

    Jorge Dubcovsky

    Article

    Debernardi et al Nat Biotechnol. 2020 Oct 12. pii: 10.103

    Insert

    35S::vitis miR396-resistant GRF4-GIF1 chimera
    

    Use

    Binary vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge