We narrowed to 6,523 results for: sas

1. pGEX-4T1-GST-Thrombin-TEV-EGFP-hNEMO

   Plasmid

   #199781

   Purpose

   Plasmid for the expression of GFP labelled NEMO (SMC Internal No. 1977).

   Depositor

   Sascha Martens

   Insert

   NEMO (IKBKG Human)
   

   Tags

   GST-Thrombin-TEV-EGFP

   Expression

   Bacterial

   Available Since

   May 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDJSVT86

   Plasmid

   #108965

   Purpose

   Express periplasmic, outer membrane, or secreted proteins in E. coli - OmpA signal and 6His tag

   Depositor

   Daniel Slade

   Article

   Casasanta et al J Biol Chem. 2017 Dec 8;292(49):20240-20

   Type

   Empty backbone

   Tags

   OmpA 1-27, 6His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDonor14-ShoHELIX-KanR (BO4)

   Plasmid

   #181795

   Purpose

   pDonor for ShoHELIX containing 14bp of spacing between the I-AniI site and LE/RE using ShCAST flanking sequence and encoding a kanamycin resistance gene as cargo

   Depositor

   Benjamin Kleinstiver

   Article

   Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   KanR, R6K origin of replication
   

   Expression

   Bacterial

   Available Since

   March 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDonor-LoxP

   Plasmid

   #219436

   Purpose

   CRISPR-associated transposases from V. cholerae and used for RNA guided cargo insertion in Vibrio natriegens

   Depositor

   Sheng Yang

   Article

   Xu et al Biotechnol Bioeng. 2024 Mar;121(3):1163-

   Insert

   two transposon ends, LoxP-terminator-LoxP gene cassette
   

   Expression

   Bacterial

   Available Since

   June 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAcCAST-sgRNA_entry (CJT83)

   Plasmid

   #181785

   Purpose

   Expresses AcCAST. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

   Depositor

   Benjamin Kleinstiver

   Article

   Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   AcTnsB, AcTnsC, AcTniQ, AcCas12k
   

   Expression

   Bacterial

   Promoter

   Lac and J23119

   Available Since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMOD_A-CmYLCV-dCas9-24XGP41

   Plasmid

   #202009

   Purpose

   Moclo level 1 - Module A, Promoter: CmYLCV, Gene: dCas9-24XGP41, Terminator: HSP

   Depositor

   Michael Smanski

   Article

   Casas-Mollano et al Nucleic Acids Research, Volume 51, Issue

   Insert

   dCas9-24XGP41
   

   Use

   Synthetic Biology; Moclo level 1 vector

   Tags

   GS linkers

   Promoter

   CmYLCV

   Available Since

   July 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pShoCAST-sgRNA_entry (BO1)

   Plasmid

   #181786

   Purpose

   Expresses ShoCAST. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

   Depositor

   Benjamin Kleinstiver

   Article

   Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   ShoTnsB, ShoTnsC, ShoTniQ, ShoCas12k
   

   Expression

   Bacterial

   Promoter

   Lac and J23119

   Available Since

   March 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRibo-Tt

   Plasmid

   #154124

   Purpose

   Expresses wt Ribo-T rRNA and a set of tRNAs in bacterial cells

   Depositor

   Alexander Mankin

   Article

   Aleksashin et al Nat Commun. 2020 Apr 20;11(1):1858. doi:

   Inserts

   engineered bacterial rRNAs operon under control of pL promotor
   bacterial tRNAs (Asp, Trp, Ile, Ala, Glu) operon under control of Ptac promoter
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   Ptac and pL

   Available Since

   Oct. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGTPA-lb

   Plasmid

   #171528

   Purpose

   Plasmid contains the Cas12k gRNA for mutant library construction

   Depositor

   Quanjiang Ji

   Article

   Chen et al Cell Rep. 2021 Aug 31;36(9):109635. doi:

   Insert

   Cas12k gRNA
   

   Use

   CRISPR

   Promoter

   Constitutive promoter J23119

   Available Since

   Aug. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGTPA

    Plasmid

    #171525

    Purpose

    Plasmid contains the Cas12k gRNA and transposon donor cassette

    Depositor

    Quanjiang Ji

    Article

    Chen et al Cell Rep. 2021 Aug 31;36(9):109635. doi:

    Insert

    Cas12k gRNA, transposon donor cassette
    

    Use

    CRISPR

    Promoter

    Constitutive promoter J23119

    Available Since

    Aug. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMOD_A-AtUBI10-dCas9-24XGP41

    Plasmid

    #202011

    Purpose

    Moclo level 1 - Module A, Promoter: AtUBI10, Gene: dCas9-24XGP41, Terminator: HSP

    Depositor

    Michael Smanski

    Article

    Casas-Mollano et al Nucleic Acids Research, Volume 51, Issue

    Insert

    dCas9-24XGP41
    

    Use

    Synthetic Biology; Moclo level 1 vector

    Promoter

    AtUBI10

    Available Since

    July 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV(CAT)T7-SB100

    Plasmid

    #34879

    Depositor

    Zsuzsanna Izsvak

    Article

    Mates et al Nat Genet. 2009 Jun;41(6):753-61. Epub 2

    Insert

    SB100X transposase
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 6, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-evoCAST-evoTnsAB

    Plasmid

    #234729

    Purpose

    Evolved human-codon optimized PseTnsAB in mammalian cells (for evoCAST)

    Depositor

    David Liu

    Article

    Witte et al Science. 2025 May 15;388(6748):eadt5199.

    Insert

    evoPseTnsAB
    

    Expression

    Mammalian

    Available Since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-evoCAST-evoTnsC

    Plasmid

    #234730

    Purpose

    Evolved human-codon optimized PseTnsC in mammalian cells (for evoCAST)

    Depositor

    David Liu

    Article

    Witte et al Science. 2025 May 15;388(6748):eadt5199.

    Insert

    evoPseTnsC
    

    Expression

    Mammalian

    Available Since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-evoCAST-TniQ

    Plasmid

    #234728

    Purpose

    Engineered human-codon optimized PseTniQ in mammalian cells (for evoCAST)

    Depositor

    David Liu

    Article

    Witte et al Science. 2025 May 15;388(6748):eadt5199.

    Insert

    PseTniQ
    

    Expression

    Mammalian

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. 3XFlag-pA-Tn5-Fl

    Plasmid

    #124601

    Purpose

    Expresses protein A and Tn5 Transposase fusion protein in bacterial cells

    Depositor

    Steven Henikoff

    Article

    Kaya-Okur et al Nat Commun. 2019 Apr 29;10(1):1930. doi:

    Insert

    Protein A and hyperactive Tn5 transposase (Tnp) fusion protein
    

    Tags

    Protein A and Tn5 transposase fusion protein with…

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-wtTnsC

    Plasmid

    #234732

    Purpose

    Wild-type human-codon optimized PseTnsC in mammalian cells

    Depositor

    David Liu

    Article

    Witte et al Science. 2025 May 15;388(6748):eadt5199.

    Insert

    PseTnsC
    

    Expression

    Mammalian

    Available Since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pShHELIX_sgRNA_entry (CJT32)

    Plasmid

    #181782

    Purpose

    Expresses ShHELIX containing a nicking I-AniI fusion to ShTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    nAniI-ShTnsB, ShTnsC, ShTniQ, ShCas12k
    

    Expression

    Bacterial

    Mutation

    nAniI = K227M, F80K, L232K

    Promoter

    Lac and J23119

    Available Since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-wtTnsAB

    Plasmid

    #234731

    Purpose

    Wild-type human-codon optimized PseTnsAB in mammalian cells

    Depositor

    David Liu

    Article

    Witte et al Science. 2025 May 15;388(6748):eadt5199.

    Insert

    PseTnsAB
    

    Expression

    Mammalian

    Available Since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAcHELIX-sgRNA_entry (CJT94)

    Plasmid

    #181783

    Purpose

    Expresses AcHELIX containing a nicking I-AniI fusion to AcTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    nAniI-AcTnsB, AcTnsC, AcTniQ, AcCas12k
    

    Expression

    Bacterial

    Mutation

    nAniI = K227M, F80K, L232K

    Promoter

    Lac and J23119

    Available Since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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