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Showing: 6751 - 6780 of 8073 results

  1. pYSD061

    Plasmid
    #212915
    Purpose
    Encodes the S. cerevisiae SUC2 (Invertase) protein (minus signal peptide) as a Type 3b' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)
    Depositor
    Paul Young
    Article
    O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102
    Insert
    SUC2 (SUC2 Budding Yeast)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    212915-plasmid-map-sequence-id-421750

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  2. pYSD022

    Plasmid
    #212908
    Purpose
    Encodes an engineered S. cerevisiae MFalpha1 pre-pro- signal peptide (MFappOPT) as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)
    Depositor
    Paul Young
    Article
    O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102
    Insert
    MFalpha1 (MF(ALPHA)1 Budding Yeast)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    212908-plasmid-map-sequence-id-421725

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  3. pYSD009

    Plasmid
    #212906
    Purpose
    Encodes S. cerevisiae SRL1 pre- signal peptide as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)
    Depositor
    Paul Young
    Article
    O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102
    Insert
    SRL1 (SRL1 Budding Yeast)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    212906-plasmid-map-sequence-id-421720

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  4. pYSD100

    Plasmid
    #212924
    Purpose
    Encodes the S. cerevisiae SUC2 (Invertase) protein (Part3) without any signal peptide assembled with pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.
    Depositor
    Paul Young
    Article
    O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102
    Insert
    SUC2 (SUC2 Budding Yeast)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Feb. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    212924-plasmid-map-sequence-id-421716

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  6. pUC57-ITR2-H1- sgCTG Merry-eCMV-SaSpD10A+linker (Neuron codon optimization)-3XFLAG-SV40 NLS-ITR2

    Plasmid
    #210736
    Purpose
    Coding for SaSp D10A Cas9 alongside Merry sgRNA targeting CAG repeats
    Depositor
    Vincent Dion
    Article
    Mangin et al bioRxiv 2023.10.24.563270
    Inserts
    SaSp D10A Cas9
    Merry sgCAG
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, PolyA signal
    Expression
    Mammalian
    Mutation
    D10A, N-terminal Sa Cas9, C-terminal Sp Cas9
    Promoter
    H1 and eCMV
    Available since
    Jan. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    210736-plasmid-map-sequence-id-420081

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  7. pUC57-ITR2-H1- sgCTG Gollum-eCMV-SaSpD10A (Neuron codon optimization)-3XFLAG-SV40 NLS-ITR2

    Plasmid
    #210735
    Purpose
    Coding for SaSp D10A Cas9 alongside Gollum sgRNA targeting CAG repeats
    Depositor
    Vincent Dion
    Article
    Mangin et al bioRxiv 2023.10.24.563270
    Inserts
    SaSp D10A Cas9
    Gollum sgCAG
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, PolyA signal
    Expression
    Mammalian
    Mutation
    D10A, N terminal Sa Cas9, C-terminal Sp Cas9
    Promoter
    H1 and eCMV
    Available since
    Jan. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    210735-plasmid-map-sequence-id-420080

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  14. nPPARg429-gs10-NLS-VPR

    Plasmid
    #188266
    Purpose
    Plasmid ensures constitutive expression of the N-terminal fragment of split PPARgamma (aa 200-429), fused to the VPR activation domain, a flexible GS linker and the SV40 NLS signal at the C-terminus
    Depositor
    Roman Jerala
    Article
    Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038
    Insert
    nPPARg429 (PPARG Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    188266-plasmid-map-sequence-id-374531

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  16. pDR111_mannose_Bgal NLS

    Plasmid
    #188398
    Purpose
    Expresses beta-galactosidase by D-mannose promoter for Bacillus subtilis. There is a secretion peptide (PhoD) and nuclear localization signal (SV40) that is fused to beta-galactosidase.
    Depositor
    Christopher Contag
    Article
    Madsen et al Commun Biol. 2022 Aug 30;5(1):888. doi:
    Insert
    lacZ
    Use
    Tags
    PhoD secretion peptide and SV40 NLS
    Expression
    Bacterial
    Mutation
    Promoter
    D-mannose
    Available since
    Aug. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    188398-plasmid-map-sequence-id-370473

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  24. CDS12-MpER-Targ

    Plasmid
    #136094
    Purpose
    CDS12 with ER targeting peptide to fuse with CTAG containing HDEL ER retention signal at the 3'end
    Depositor
    Jim Haseloff
    Article
    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102
    Insert
    ER targeting peptide from predicted chitinase Mapoly0069s0092
    Use
    Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    BsaI/ SapI domesticated
    Promoter
    Available since
    Nov. 30, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    136094-plasmid-map-sequence-id-275025

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  25. pDONR207-MUM2sp-PelA

    Plasmid
    #170722
    Purpose
    Gateway (Invitrogen) entry clone (pDONR207) containing the MUM2 (At5g63800) signal peptide (84bp) fused in-frame to the coding sequence of the HG degrading enzyme PelA (AN2331.2)
    Depositor
    George Haughn
    Article
    McGee et al Plant Cell Physiol. 2021 Jun 1. pii: 629
    Insert
    Pectin lyase A
    Use
    Gateway donor vector / entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 20, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    170722-plasmid-map-sequence-id-345737

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  26. pDONR207-MUM2sp-RglB

    Plasmid
    #170721
    Purpose
    Gateway (Invitrogen) entry clone (pDONR207) containing the MUM2 (At5g63800) signal peptide (84bp) fused in-frame to the coding sequence of the RG-I degrading enzyme RglB (AN6395.2)
    Depositor
    George Haughn
    Article
    McGee et al Plant Cell Physiol. 2021 Jun 1. pii: 629
    Insert
    Rhamnogalacturonan lyase B
    Use
    Gateway donor vector / entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 20, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    170721-plasmid-map-sequence-id-345736

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  27. pDONR207-MUM2sp-RglA

    Plasmid
    #170720
    Purpose
    Gateway (Invitrogen) entry clone (pDONR207) containing the MUM2 (At5g63800) signal peptide (84bp) fused in-frame to the coding sequence of the RG-I degrading enzyme RglA (AN7135.2)
    Depositor
    George Haughn
    Article
    McGee et al Plant Cell Physiol. 2021 Jun 1. pii: 629
    Insert
    Rhamnogalacturonan lyase A
    Use
    Gateway donor vector / entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 20, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    170720-plasmid-map-sequence-id-345734

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  29. pLyGo-Bs-4

    Plasmid
    #163142
    Purpose
    pLyGo cloning vector for a sequence of interest (LPMO) in B. subtilis. Vector encoding the BatLPMO10 signal peptide and the LyGo cassette (SapI-ccdB-SapI)
    Depositor
    Morten Norholm
    Article
    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PamyL-PamyQ-PcryIIIA-cryIIIAstab49
    Available since
    July 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    163142-plasmid-map-sequence-id-338663

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  30. pLyGo-Ec-4

    Plasmid
    #163130
    Purpose
    pLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding PhoA signal peptide and the LyGo cassette (SapI-ccdB-SapI)
    Depositor
    Morten Norholm
    Article
    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    July 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    163130-plasmid-map-sequence-id-338661

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Showing: 6751 - 6780 of 8073 results