We narrowed to 11,766 results for: nsf

1. pCAGGS-FuG-B

   Plasmid

   #67523

   Purpose

   This is an envelope gene encoding plasmid to make retrogradely lentiviral vector. You can make type B envelope coating virus particle with HiRet by using this plasmid.

   Depositor

   Kazuto Kobayashi

   Article

   Kato et al Hum Gene Ther. 2011 Feb;22(2):197-206. d

   Insert

   Fusion Glycoprotein type B
   

   Use

   Lentiviral

   Tags

   Fusion protein of Vesicular stomatitis Indiana vi…

   Promoter

   CAGGS

   Available Since

   July 31, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pDN-D2irTN6kwh

   Plasmid

   #44724

   Depositor

   Gabor Balazsi

   Article

   Nevozhay et al Nat Commun. 2013 Feb 5;4:1451. doi: 10.1

   Inserts

   pCMV-D2i promoter
   htetR::NLS
   

   Use

   Synthetic Biology; Expression regulator/reporter;…

   Tags

   Rabbit β-globin intron II and WPRE

   Expression

   Mammalian

   Mutation

   Initiator motif (Inr) displaced relative to pCMV-…

   Promoter

   pCMV-D2i

   Available Since

   April 23, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAAV-syn-FLEX-axon-jYCaMP1s

   Plasmid

   #135421

   Purpose

   Axon-targeted yellow protein calcium sensor expressed under human Synapsin1 promoter, Cre mediated flip-excision switch, for AAV production or direct transfection, slow variant

   Depositor

   Kaspar Podgorski

   Article

   Mohr et al Nat Methods. 2020 May 25. pii: 10.1038/s

   Has Service

   AAV1

   Insert

   jYCaMP1s
   

   Use

   AAV

   Tags

   GAP43 axon targeting sequence

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   Jan. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDam-Myc-HP1

   Plasmid

   #59221

   Purpose

   expresses Dam-myc-HP1

   Depositor

   Bas van Steensel

   Article

   van Steensel et al Nat Biotechnol. 2000 Apr;18(4):424-8.

   Insert

   HP1 (Su(var)205 Fly)
   

   Use

   Damid

   Tags

   Dam and myc

   Expression

   Insect

   Promoter

   Drosophila heat shock promoter

   Available Since

   May 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCCL-PGK-SPdCas9-BFP-DNMT3A(E752A)

   Plasmid

   #71213

   Purpose

   dCas9 fused to BFP and the human DNMT3A catalytically inactive domain

   Depositor

   Yonglun Luo

   Article

   Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

   Insert

   dSpCas9-BFP-DNMT3A(E752A), DNMT3A catalytic domain with inactivation mutation E752A
   

   Tags

   dSpCas9-BFP-DNMT3A(E752A)

   Expression

   Mammalian

   Available Since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pJHG003

   Plasmid

   #126454

   Purpose

   Expresses Cas9-XTEN-dTdT in mammalian cells.

   Depositor

   Chang Liu

   Article

   Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

   Insert

   Cas9-XTEN-dTdT (DNTT Streptococcus pyogenes, Human)
   

   Expression

   Mammalian

   Mutation

   Added D343E and D345E to inactivate TdT.

   Promoter

   CMV

   Available Since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSB65 - pL0_tGFP-I (CDS1)

   Plasmid

   #123182

   Purpose

   Golden Gate (MoClo; CDS1) compatible turbo GFP gene from P. plumata with an intron for transient and stable expression in plants (moved to a CDS1 position from pICSL50016; Engler et al., 2014)

   Depositor

   Erin Cram, Carolyn Lee-Parsons

   Article

   Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

   Insert

   turboGFP codon-optimised for plants (P. plumata) with intron, U5 small nuclear ribonucleoprotein component (A. thaliana)
   

   Use

   Part for plant expression

   Expression

   Plant

   Mutation

   No BpiI and BsaI sites; codon-optimised for plants

   Available Since

   May 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBiex1-SYNAC

   Plasmid

   #82710

   Purpose

   Synthetic soluble adenylyl cyclase FRET sensor and cAMP generator

   Depositor

   Sivaraj Sivaramakrishnan

   Article

   Ritt et al Mol Pharmacol. 2016 Apr;89(4):407-12. do

   Insert

   SYNAC (ADCY2, ADCY5 Human)
   

   Tags

   FLAG, mCerulean, and mCitrine

   Expression

   Bacterial and Insect

   Promoter

   T7

   Available Since

   Nov. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pNIA-CEN-mVenus-FLAG-LANS1-Set2

   Plasmid

   #122003

   Purpose

   mVenus-FLAG-LANS-Set2: mVenus- and FLAG-tagged LANS1-Set2 for light control of Set2 localization in yeast

   Depositor

   Brian Kuhlman

   Article

   Lerner et al Genome Res. 2020 Nov;30(11):1605-1617. d

   Insert

   mVenus-FLAG-LANS1-Set2 (SET2 Budding Yeast)
   

   Use

   Synthetic Biology

   Tags

   mVenus-FLAG

   Expression

   Yeast

   Mutation

   Set2 point mutations K538G, K539S, R549G, K550S, …

   Promoter

   ADH1

   Available Since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCMV CHMP2B-L4D F5D-3XHA

    Plasmid

    #232003

    Purpose

    Expression of a CHMP2B membrane binding mutant (L4D F5D) with a 3xHA tag.

    Depositor

    Franz-Ulrich Hartl

    Article

    Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

    Insert

    CHMP2B (CHMP2B Human)
    

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    L4D F5D

    Available Since

    Aug. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pEF1a FLAG-CHMP2B-d55-96

    Plasmid

    #232013

    Purpose

    Expression of FLAG-tagged CHMP2B with the second alpha helix deleted.

    Depositor

    Franz-Ulrich Hartl

    Article

    Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

    Insert

    CHMP2B (CHMP2B Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Δ55-96

    Available Since

    Aug. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSPcas9(BB)-2A-Puro V2.0 sgCHMP2B-2 aauucccaaaugaagauggc

    Plasmid

    #231998

    Purpose

    Expression of an sgRNA targeting CHMP2B, Cas9, and a PuroR marker

    Depositor

    Franz-Ulrich Hartl

    Article

    Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

    Insert

    CHMP2B-targeted sgRNA (CHMP2B Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Aug. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

13. pAAV-EF1α-DIO-cOpn5-T2A-EGFP

    Plasmid

    #237858

    Purpose

    The transfer plasmid for packaging AAV expressing Cre-dependent chicken OPN5 was constructed by inserting the OPN5 coding sequence into the multiple cloning site under the control of EF1α promoter

    Depositor

    Minmin Luo

    Article

    Dai et al Sci China Life Sci. 2022 Jul;65(7):1271-

    Insert

    Opn5 (OPN5 Chicken)
    

    Use

    AAV and Cre/Lox

    Promoter

    EF1α

    Available Since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV-EF1α-DIO-cOpn5-T2A-mcherry

    Plasmid

    #238009

    Purpose

    The transfer plasmid for packaging AAV expressing Cre-dependent chicken OPN5 was constructed by inserting the OPN5 coding sequence into the multiple cloning site under the control of EF1α promoter

    Depositor

    Minmin Luo

    Article

    Dai et al Sci China Life Sci. 2022 Jul;65(7):1271-

    Insert

    Opn5 (OPN5 Chicken)
    

    Use

    AAV and Cre/Lox

    Promoter

    EF1α

    Available Since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. AAV-CMV-scFVDnmt3A_bGHpA

    Plasmid

    #177349

    Purpose

    AAV expression of scFV-fused catalytic domain of Dnmt3a for targeted DNA methylation editing

    Depositor

    Naoki Yamamoto

    Article

    Yamamoto et al Research Square [Preprint] 2021. doi: 10

    Insert

    catalytic domain of DNA Methyltransferase 3 Alpha (Dnmt3a Mouse)
    

    Use

    AAV

    Tags

    myc and scFV

    Expression

    Mammalian

    Mutation

    598–908 aa of mouse Dnmt3a

    Promoter

    CMV

    Available Since

    Sept. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pKG-GW1-hCIT K42E

    Plasmid

    #203168

    Purpose

    Yeast expression vector for human DHDDS K42E

    Depositor

    William Sessa

    Article

    Park et al Cell Metab. 2014 Sep 2;20(3):448-57. doi

    Insert

    DHDDS (DHDDS Human)
    

    Expression

    Yeast

    Mutation

    K42E

    Promoter

    TDH3

    Available Since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pT22UbhaEng2aE4PCh(#271)

    Plasmid

    #184085

    Purpose

    cyclofen-inducible fish Eng2a (wt) activation in zebrafish permanent transgenic

    Depositor

    Sophie Vriz

    Article

    Rampon et al Development. 2015 May 15;142(10):1840-9.

    Insert

    3HA-ZfEng2a-ERT2-P2A-mCherry
    

    Use

    Zebrafish transgenesis (blue eyes)

    Tags

    3xHA

    Mutation

    ERT2: G400V, M543A, L544A, deleted 1-281;

    Promoter

    Zf-Ubi

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pT22UbhaEng2bE4PCh(#272)

    Plasmid

    #184086

    Purpose

    cyclofen-inducible fish Eng2b (wt) activation in zebrafish permanent transgenic

    Depositor

    Sophie Vriz

    Article

    Rampon et al Development. 2015 May 15;142(10):1840-9.

    Insert

    3HA-ZfEng2b-ERT2-P2A-mCherry
    

    Use

    Zebrafish transgenesis (blue eyes)

    Tags

    3xHA

    Mutation

    ERT2: G400V, M543A, L544A, deleted 1-281;

    Promoter

    Zf-Ubi

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pT22gCfpUbm5En2E4PCh(#251)

    Plasmid

    #184083

    Purpose

    cyclofen-inducible chicken EN2 (wt) activation in zebrafish permanent transgenic

    Depositor

    Sophie Vriz

    Article

    Rampon et al Development. 2015 May 15;142(10):1840-9.

    Insert

    5myc-chkEn2-ERT2-P2A-mCherry
    

    Use

    Zebrafish transgenesis (blue eyes)

    Tags

    5xMyc

    Mutation

    ERT2: G400V, M543A, L544A, deleted 1-281;

    Promoter

    Zf-Ubi

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pT22Ubm5Eng2bE4PCh(#284)

    Plasmid

    #184088

    Purpose

    cyclofen-inducible fish Eng2b (wt) activation in zebrafish permanent transgenic

    Depositor

    Sophie Vriz

    Article

    Rampon et al Development. 2015 May 15;142(10):1840-9.

    Insert

    5myc-ZfEng2b-ERT2-P2A-mCherry
    

    Use

    Zebrafish transgenesis (blue eyes)

    Tags

    5xMyc

    Mutation

    ERT2: G400V, M543A, L544A, deleted 1-281;

    Promoter

    Zf-Ubi

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge