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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 5,331 results for: pcdna3.1

Showing: 4501 - 4520 of 5331 results

  1. Luc-PS9

    Plasmid
    #177942
    Purpose
    Transient mammalian expression of Luc2 (firefly luciferase) along with SARS-CoV-2 sequence 20080-21171 (PS9) within the 3'UTR. Resulting transcript is packaged into SARS-CoV-2 virus-like particles.
    Depositor
    Jennifer Doudna
    Article
    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11
    Insert
    Luc2 (ORF1ab )
    Expression
    Mammalian
    Available Since
    Nov. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    177942-plasmid-map-sequence-id-348206

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  2. CaMK2rep-S603A

    Plasmid
    #239623
    Purpose
    Inactive CaMKII activity reporter. The plasmid contains sGFP2 fluorescent protein, 3xmyc tags and double mutated phospho-site 3 from rat Synapsin-1a.
    Depositor
    F Javier Díez Guerra
    Article
    Martinez-Blanco et al Anal Chem. 2025 Sep 23;97(37):20275-2029
    Insert
    SpotNES-sGFP2-SynP3-3xmyc (Syn1 Rat, Synthetic)
    Tags
    Spot, myc, and sGFP2
    Expression
    Mammalian
    Mutation
    changed Serine 603 to Alanine
    Promoter
    CMV
    Available Since
    July 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    239623-plasmid-map-sequence-id-476237

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  4. CMV-CASANOVA

    Plasmid
    #113035
    Purpose
    CASANOVA (AcrIIA4-LOV2 hybrid) expression in mammalian cells; enables optogenetic control of SpyCas9
    Depositor
    Dominik Niopek
    Article
    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s
    Insert
    CASANOVA (for CRISPR/Cas9 activity switching via a novel optogenetic variant of AcrIIA4)
    Use
    CRISPR and Synthetic Biology
    Expression
    Mammalian
    Available Since
    Oct. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    113035-plasmid-map-sequence-id-224728

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  9. CoV2-Spike-D614G

    Plasmid
    #177960
    Purpose
    Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (original Wuhan Hu1 sequence + D614G mutation)
    Depositor
    Jennifer Doudna
    Article
    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11
    Insert
    SARS-CoV-2 Spike protein (original Wuhan Hu1 sequence + D614G mutation) (S )
    Expression
    Mammalian
    Mutation
    D614G
    Available Since
    Nov. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    177960-plasmid-map-sequence-id-348179

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  12. Luc-T20

    Plasmid
    #177941
    Purpose
    Transient mammalian expression of Luc2 (firefly luciferase) along with SARS-CoV-2 sequence 20080-22222 (T20) within the 3'UTR. Resulting transcript is packaged into SARS-CoV-2 virus-like particles.
    Depositor
    Jennifer Doudna
    Article
    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11
    Insert
    Luc2 (ORF1ab )
    Expression
    Mammalian
    Available Since
    Nov. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    177941-plasmid-map-sequence-id-348207

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  18. CMV-mito-GEM-GECO1

    Plasmid
    #32461
    Purpose
    Mammalian expression of mitochondria-targeting blue-green emission ratiometric genetically encoded Ca2+ indicator for optical imaging
    Depositor
    Robert Campbell
    Article
    Zhao et al Science. 2011 Sep 30;333(6051):1888-91.
    Insert
    GEM-GECO
    Tags
    a duplex of the mitochondrial targeting signal of…
    Expression
    Mammalian
    Mutation
    GCaMP3 L60P/K69E/N77Y/D86G/N98I/K119I/L173Q/T223S…
    Promoter
    CMV
    Available Since
    Dec. 5, 2011
    Availability
    Academic Institutions and Nonprofits only
    32461-plasmid-map-sequence-id-123705

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  19. NanoLuc-PM

    Plasmid
    #164784
    Purpose
    Nanoluciferase (NanoLuc) targeted to the extracellular surface of the cell membrane for bioluminescence resonance energy transfer (BRET)-based measurement of ligand binding to unmodified receptors
    Depositor
    Laszlo Hunyady
    Article
    Toth et al J Biol Chem. 2021 Feb 2:100366. doi: 10.
    Insert
    Nanoluciferase fused with the transmembrane domain of the platelet-derived growth factor receptor beta (PDGFRB Human, Oplophorus gracilirostris)
    Tags
    NanoLuc
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    May 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    164784-plasmid-map-sequence-id-326673

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Showing: 4501 - 4520 of 5331 results