We narrowed to 10,330 results for: plasmids 101

1. pET26b_Nb.AT110

   Plasmid

   #135480

   Purpose

   E. coli periplasmic expression of conformational nanobody Nb.AT110 recognizing angiotensin II type 1 receptor

   Depositor

   Andrew Kruse

   Article

   Wingler et al Cell. 2019 Jan 24;176(3):479-490.e12. do

   Insert

   Nanobody AT110
   

   Use

   Tags

   6xHis-tag and pelB leader

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Oct. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAV-CAG-LSL-mCherry-shLuci

   Plasmid

   #178590

   Purpose

   Cre-dependent expression of mCherry and a shRNA against luciferase under the constitutive promoter

   Depositor

   Chun-Li Zhang

   Article

   Wang et al Cell. 2021 Oct 14;184(21):5465-5481.e16.

   Insert

   miRE-Luciferase
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDB007ns2a

   Plasmid

   #99214

   Purpose

   Accessory plasmid for PACE of PylRS variants; negative selection

   Depositor

   David Liu

   Article

   Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

   Insert

   Ppsp [SD8] gIII; PproK tyrT(Opt,CUA); Ptet [SD4] T7RNAP(S12*,S203*)
   

   Use

   Tags

   Expression

   Mutation

   amber codons at positions 12 and 203 of T7 RNA po…

   Promoter

   Ppsp, PproK, and Ptet

   Available Since

   Oct. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGL2_168

   Plasmid

   #199100

   Purpose

   Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

   Depositor

   Charlie Gilbert, Cultivarium Tools, Nili Ostrov

   Article

   Gilbert et al bioRxiv 2023.05.19.541510

   Insert

   RSF1010 ORI plus NGS barcode 14, gentamicin resistance cassette, mScarlet-I cassette, oriT
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   June 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. APneg-SNAP25d

   Plasmid

   #168014

   Purpose

   negative selection PACE Accessory Plasmid

   Depositor

   David Liu

   Article

   Blum et al Science. 2021 Feb 19;371(6531):803-810.

   Insert

   SNAP25-T7PAP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   May 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pNR291

   Plasmid

   #79555

   Purpose

   Output plasmid: contains 5-state register that regulates expression of GFP, RFP, and BFP. Expresses a unique fluorescent signature in each state.

   Depositor

   Timothy Lu

   Article

   Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

   Inserts

   GFPmut3b
   mTagBFP
   mRFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. TC512

   Plasmid

   #122615

   Purpose

   Pmig-13::Zip(-)::QFAD::NLS::SL2::mCherry

   Depositor

   Tian Chi

   Article

   Mao et al iScience. 2019 Jan 25;11:224-237. doi: 1

   Insert

   Zip(-)::QFAD::NLS::SL2::mCherry
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available Since

   May 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-nEF-ConVERGD-eGFP-WPRE

   Plasmid

   #218747

   Purpose

   Provides AND intersectional (Cre+Flp) expression of eGFP

   Depositor

   Lindsay Schwarz

   Article

   Hughes et al bioRxiv. 2023 Feb 8:2023.02.07.527312. d

   Insert

   eGFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   nEF

   Available Since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-nEF-ConVERGD-eGFP-W3SL

   Plasmid

   #218746

   Purpose

   Provides AND intersectional (Cre+Flp) expression of eGFP

   Depositor

   Lindsay Schwarz

   Article

   Hughes et al bioRxiv. 2023 Feb 8:2023.02.07.527312. d

   Insert

   eGFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   nEF

   Available Since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. APT3-VAMP7

    Plasmid

    #168002

    Purpose

    positive selection PACE Accessory Plasmid

    Depositor

    David Liu

    Article

    Blum et al Science. 2021 Feb 19;371(6531):803-810.

    Insert

    VAMP7-T3'PAP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. APneg-SNAP25c

    Plasmid

    #168013

    Purpose

    negative selection PACE Accessory Plasmid

    Depositor

    David Liu

    Article

    Blum et al Science. 2021 Feb 19;371(6531):803-810.

    Insert

    SNAP25-T7PAP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. APneg-SNAP25b

    Plasmid

    #168012

    Purpose

    negative selection PACE Accessory Plasmid

    Depositor

    David Liu

    Article

    Blum et al Science. 2021 Feb 19;371(6531):803-810.

    Insert

    SNAP25-T7PAP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. AP-PTEN(med)

    Plasmid

    #168005

    Purpose

    positive selection PACE Accessory Plasmid

    Depositor

    David Liu

    Article

    Blum et al Science. 2021 Feb 19;371(6531):803-810.

    Insert

    PTEN-T7PAP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. mCh-LaGFP(N)-cpFRB2-T2A-FKBP-LaGFP(C)

    Plasmid

    #165030

    Purpose

    Express cpFRB2-based GFP specific Chessbody (chemically switchable split nanobody); visualized by mCherry

    Depositor

    Yubin Zhou

    Article

    Lee et al J Mol Biol. 2020 May 1;432(10):3127-3136

    Insert

    LaGFP(N)-cpFRB2-T2A-FKBP-LaGFP(C)
    

    Use

    Tags

    mCherry

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    T7

    Available Since

    May 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pARv-RFP

    Plasmid

    #60021

    Purpose

    pARv-RFP is a fluorescent reporter plasmid, that allows fast and easy evaluation of ZFN, TALEN or CRISPR/Cas9 activity in the transfected cells.

    Depositor

    Radislav Sedláček

    Article

    Kasparek et al FEBS Lett. 2014 Sep 18. pii: S0014-5793(

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGL2_165

    Plasmid

    #199097

    Purpose

    Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

    Depositor

    Charlie Gilbert, Cultivarium Tools, Nili Ostrov

    Article

    Gilbert et al bioRxiv 2023.05.19.541510

    Insert

    pSC101ts ORI plus NGS barcode 10, gentamicin resistance cassette, mScarlet-I cassette, oriT
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pZac2.1 hSynapsin1 NAPA-N2 SV40

    Plasmid

    #97212

    Purpose

    FRET probe; extracelllular synapse targeted mScarlet

    Depositor

    Baljit Khakh

    Article

    Octeau et al Neuron. 2018 Apr 4;98(1):49-66.e9. doi:

    Insert

    extracellular membrane tethered fluorescent protein
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSynapsin1

    Available Since

    Nov. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pZmU3-gRNA

    Plasmid

    #53061

    Purpose

    Transcription of guide RNA (gRNA) in maize cells

    Depositor

    Caixia Gao

    Article

    Liang et al J Genet Genomics. 2014 Feb 20;41(2):63-8

    Insert

    zmU3 promoter - gRNA
    

    Use

    CRISPR; Plant expression

    Tags

    Expression

    Mutation

    Promoter

    maize U3

    Available Since

    Aug. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. IRE-miR21-SunTag-TREAT

    Plasmid

    #196932

    Purpose

    SunTag cassette was added in the coding region in the miR21-IRE-TREAT reporter.

    Depositor

    Jeffrey Chao

    Article

    Dave et al Mol Cell. 2023 Feb 16;83(4):589-606.e6.

    Insert

    Renilla luciferase
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available Since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. AP-VAMP1

    Plasmid

    #167996

    Purpose

    positive selection PACE Accessory Plasmid

    Depositor

    David Liu

    Article

    Blum et al Science. 2021 Feb 19;371(6531):803-810.

    Insert

    VAMP1(T29-K89)-T7PAP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge