We narrowed to 12,684 results for: sequence

1. pT7-EGFP-C1-HsR3HCC1_39-480-EBMmut_K

   Plasmid

   #146765

   Purpose

   Mammalian Expression of HsR3HCC1_39-480-EBMmut

   Depositor

   Elisa Izaurralde

   Article

   Kashima et al Genes Dev. 2010 Nov 1. 24(21):2440-50.

   Insert

   HsR3HCC1_39-480-EBMmut (R3HCC1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   sequence extracted from BC050572.1; 39-480 trunca…

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pT7-EGFP-C1-HsGIGYF1_1-177_AE

   Plasmid

   #148570

   Purpose

   Mammalian Expression of HsGIGYF1_1-177

   Depositor

   Elisa Izaurralde

   Article

   Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

   Insert

   HsGIGYF1_1-177 (GIGYF1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pT7-EGFP-C1-HsGIGYF1-Y39AY41AM46AL47A_AE

   Plasmid

   #148576

   Purpose

   Mammalian Expression of HsGIGYF1-Y39AY41AM46AL47A

   Depositor

   Elisa Izaurralde

   Article

   Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

   Insert

   HsGIGYF1-Y39AY41AM46AL47A (GIGYF1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pT7-EGFP-HsGIGYF1_1-177-LFVENtoDDDAF_AE

   Plasmid

   #148581

   Purpose

   Mammalian Expression of HsGIGYF1_1-177-LFVENtoDDDAF

   Depositor

   Elisa Izaurralde

   Article

   Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

   Insert

   HsGIGYF1_1-177-LFVENtoDDDAF (GIGYF1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pENT-L1-Kozak-Ark-L2

   Plasmid

   #186362

   Purpose

   Entry clone with ORF encoding Ciona robusta Aurora kinase A flanked by Gateway recombination sequences. No Stop sequence: to be recombined into a destination vector with a C-terminal protein tag.

   Depositor

   Patrick Lemaire

   Article

   Roure et al PLoS One. 2007. 2(9):e916.

   Insert

   Ciona robusta Aurora kinase A
   

   Use

   Expression of a fluorescent centrosome marker

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. KTK_139

   Plasmid

   #180565

   Purpose

   Spacer sequence in D1.1 format. Allows for Level 2 cloning with D1.2

   Depositor

   Tom Ellis

   Article

   Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

   Insert

   Spacer sequence in D1.1 format. Allows for Level 2 cloning with D1.2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. KTK_113

   Plasmid

   #180540

   Purpose

   Entry vector containing Signal peptide region of CsgA including SecB-N22 sequenced

   Depositor

   Tom Ellis

   Article

   Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

   Insert

   Signal peptide region of CsgA including SecB-N22 sequenced
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJCC_162 SpCas9 KES(1107-1109)GG

   Plasmid

   #179526

   Purpose

   For bacterial expression of SpCas9 KES(1107-1109)GG (phosphate lock loop mutant) with an N-terminal His-MBP tag

   Depositor

   Jennifer Doudna

   Article

   Cofsky et al Nat Struct Mol Biol. 2022 Apr;29(4):395-

   Insert

   SpCas9 KES(1107-1109)GG
   

   Use

   Tags

   10X His, TEV protease cleavage site, and maltose-…

   Expression

   Bacterial

   Mutation

   replaced KES (residues 1107-1109) with GG

   Promoter

   Available since

   April 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJCC_163 SpCas9 K233A/K234A/K253A/K263A

   Plasmid

   #179527

   Purpose

   For bacterial expression of SpCas9 K233A/K234A/K253A/K263A (helix-rolling basic patch mutant) with an N-terminal His-MBP tag

   Depositor

   Jennifer Doudna

   Article

   Cofsky et al Nat Struct Mol Biol. 2022 Apr;29(4):395-

   Insert

   SpCas9 K233A/K234A/K253A/K263A
   

   Use

   Tags

   10X His, TEV protease cleavage site, and maltose-…

   Expression

   Bacterial

   Mutation

   changed lysines 233, 234, 253, and 263 to alanine

   Promoter

   Available since

   April 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. KTK_324

    Plasmid

    #180566

    Purpose

    Spacer sequence in 2.2b format to allow for level 3 cloning with D2.2a

    Depositor

    Tom Ellis

    Article

    Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

    Insert

    Spacer sequence in 2.2b format to allow for level 3 cloning with D2.2a
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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