Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 18,346 results for: gateway

Showing: 11301 - 11350 of 18346 results

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pDest17_A1-LCD swap X7

Plasmid

#226549

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD X7

Depositor

Tanja Mittag

Article

Pesce et al Sci Adv. 2024 Aug 30;10(35):eadm9926. do

Insert

A1-LCD swap X7

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDest17_A1-LCD swap X1

Plasmid

#226543

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD X1

Depositor

Tanja Mittag

Article

Pesce et al Sci Adv. 2024 Aug 30;10(35):eadm9926. do

Insert

A1-LCD swap X1

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDest17_A1-LCD swap V5

Plasmid

#226542

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD V5

Depositor

Tanja Mittag

Article

Pesce et al Sci Adv. 2024 Aug 30;10(35):eadm9926. do

Insert

A1-LCD swap V5

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDest17_A1-LCD swap X4

Plasmid

#226546

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD X4

Depositor

Tanja Mittag

Article

Pesce et al Sci Adv. 2024 Aug 30;10(35):eadm9926. do

Insert

A1-LCD swap X4

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDest17_A1-LCD swapX3

Plasmid

#226545

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD X3

Depositor

Tanja Mittag

Article

Pesce et al Sci Adv. 2024 Aug 30;10(35):eadm9926. do

Insert

A1-LCD swap X3

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDest17_A1-LCD swap V4

Plasmid

#226541

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD V4

Depositor

Tanja Mittag

Article

Pesce et al Sci Adv. 2024 Aug 30;10(35):eadm9926. do

Insert

A1-LCD swap V4

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDest17_A1-LCD swap X10

Plasmid

#226552

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD X10

Depositor

Tanja Mittag

Article

Pesce et al Sci Adv. 2024 Aug 30;10(35):eadm9926. do

Insert

A1-LCD swap X10

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDest17_A1-LCD swap X9

Plasmid

#226551

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD X9

Depositor

Tanja Mittag

Article

Pesce et al Sci Adv. 2024 Aug 30;10(35):eadm9926. do

Insert

A1-LCD swap X9

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDest17_A1-LCD swap X8

Plasmid

#226550

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD X8

Depositor

Tanja Mittag

Article

Pesce et al Sci Adv. 2024 Aug 30;10(35):eadm9926. do

Insert

A1-LCD swap X8

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDest17_A1-LCD swap X6

Plasmid

#226548

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD X6

Depositor

Tanja Mittag

Article

Pesce et al Sci Adv. 2024 Aug 30;10(35):eadm9926. do

Insert

A1-LCD swap X6

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDest17_A1-LCD swap X2

Plasmid

#226544

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD X2

Depositor

Tanja Mittag

Article

Pesce et al Sci Adv. 2024 Aug 30;10(35):eadm9926. do

Insert

A1-LCD swap X2

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCW57.1-p37-SHPmut-FLAG

Plasmid

#225502

Purpose

Inducible expression of FLAG-tagged mutated p37 protein

Depositor

David Rubinsztein

Article

Wrobel et al Sci Adv. 2024 May 3;10(18):eadl6082. doi

Insert

p37 (UBXN2B Human)

Use

Lentiviral; Gateway destination vector, doxycycli…

Tags

FLAG

Expression

Mammalian

Mutation

F215A/E218A/QKL220-222AAA

Promoter

TRE promoter, Tet ON

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pCW57.1-p37-WT-FLAG

Plasmid

#225501

Purpose

Inducible expression of FLAG-tagged wildtype p37 protein

Depositor

David Rubinsztein

Article

Wrobel et al Sci Adv. 2024 May 3;10(18):eadl6082. doi

Insert

p37 (UBXN2B Human)

Use

Lentiviral; Gateway destination vector, doxycycli…

Tags

FLAG

Expression

Mammalian

Mutation

no

Promoter

TRE promoter, Tet ON

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBi2FC-N-MPK6-P2A:mCherry

Plasmid

#216137

Purpose

Controls for Bicistronic BiFC

Depositor

Imran Siddiqi

Article

Sivakumar et al Physiol Mol Biol Plants. 2024 Jul;30(7):

Insert

MPK6 (MPK6 Mustard Weed)

Expression

Plant

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
p8221 pLIX-PTPN14

Plasmid

#221643

Purpose

Doxycycline-inducible overexpression of human WT PTPN14

Depositor

Elizabeth White

Article

Blakely et al mBio. 2024 Sep 9:e0181124. doi: 10.1128/

Insert

PTPN14 (PTPN14 Human)

Use

Lentiviral

Tags

HA

Available Since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pSRK51

Plasmid

#223722

Purpose

Expresses His-tagged mutant (L56V/S135G/S219V) TEV protease in E. coli

Depositor

Sreejith Raran-Kurussi

Article

Parida et al Curr Res Struct Biol. 2023 Sep 30;6:1001

Insert

TEV protease

Tags

NT* (self cleaving)

Expression

Bacterial

Mutation

L56V/S135G/S219V

Promoter

T7

Available Since

Aug. 28, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pPB-cT3G-cERP2-UBB

Plasmid

#222569

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of UBB

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

UBB (UBB Human)

Expression

Mammalian

Available Since

Aug. 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pPB-cT3G-cERP2-HDAC6

Plasmid

#222566

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of HDAC6

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

HDAC6 (HDAC6 Human)

Expression

Mammalian

Available Since

Aug. 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pPB-cT3G-cERP2-DMRT1

Plasmid

#222571

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of DMRT1

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

DMRT1 (DMRT1 Human)

Expression

Mammalian

Available Since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pPB-cT3G-cERP2-HOXA10

Plasmid

#222539

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of HOXA10

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

HOXA10 (HOXA10 Human)

Expression

Mammalian

Available Since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pPB-cT3G-cERP2-HMGB3

Plasmid

#222540

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of HMGB3

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

HMGB3 (HMGB3 Human)

Expression

Mammalian

Available Since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pPB-cT3G-cERP2-ESX1

Plasmid

#222563

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of ESX1

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

ESX1 (ESX1 Human)

Expression

Mammalian

Available Since

Aug. 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pPB-cT3G-cERP2-3xFLAG-SMAD9-active

Plasmid

#222580

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of 3xFLAG-SMAD9

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

SMAD9 (SMAD9 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

S489E, S491E

Available Since

Aug. 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-MLG-EGFP-LST2

Plasmid

#220785

Purpose

Expresses N-terminal EGFP-LST2 in mammalian cells

Depositor

Timm Maier

Article

Battaglioni et al Proc Natl Acad Sci U S A. 2024 Aug 20;12

Insert

LST2 (ZFYVE28 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
MAC3_C_VPS35L(A830T)

Plasmid

#209693

Purpose

Expresses MAC3-tagged VPS35L(A830T) in mammalian cells

Depositor

Markku Varjosalo

Article

Laulumaa et al Nat Struct Mol Biol. 2024 Jun;31(6):925-

Insert

VPS35L (VPS35L Human)

Tags

MAC3-tag

Expression

Mammalian

Mutation

A830T, changed Alanine 830 to Threonine

Available Since

Aug. 7, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
MAC_C_VPS35L

Plasmid

#209692

Purpose

Expresses MAC-tagged VPS35L in mammalian cells

Depositor

Markku Varjosalo

Article

Laulumaa et al Nat Struct Mol Biol. 2024 Jun;31(6):925-

Insert

VPS35L (VPS35L Human)

Tags

MAC-tag

Expression

Mammalian

Mutation

NC_000016.9:g.19710824G>A

Available Since

Aug. 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLX-EF1a_NKX2-1_W208L

Plasmid

#221486

Purpose

NKX2-1 W208L DNA binding mutation lentiviral overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

W208L

Promoter

EF1a

Available Since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLX-EF1a_NKX2-1_DelNTerm_160-371AA

Plasmid

#221487

Purpose

NKX2-1 N-terminal truncation mutation lentiviral overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Deletion of 1-159AA N-terminal domain

Promoter

EF1a

Available Since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLX-EF1a_NKX2-1_V205F

Plasmid

#221485

Purpose

NKX2-1 V205F DNA binding mutation lentiviral overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

V205F

Promoter

EF1a

Available Since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLX-EF1a_NKX2-1_DelCTerm_1-223AA

Plasmid

#221488

Purpose

NKX2-1 C-terminal truncation mutation lentiviral overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Deletion of 224-371AA C-terminal domain

Promoter

EF1a

Available Since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBi2FC-N-MPK5-P2A:mCherry

Plasmid

#216136

Purpose

Controls for Bicistronic BiFC

Depositor

Imran Siddiqi

Article

Sivakumar et al Physiol Mol Biol Plants. 2024 Jul;30(7):

Insert

MPK5 (MPK5 Mustard Weed)

Expression

Plant

Available Since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBi2FC-N-MPK12-P2A:mCherry

Plasmid

#216139

Purpose

Controls for Bicistronic BiFC

Depositor

Imran Siddiqi

Article

Sivakumar et al Physiol Mol Biol Plants. 2024 Jul;30(7):

Insert

MPK12 (MPK12 Mustard Weed)

Expression

Plant

Available Since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221-kcnk2b

Plasmid

#208984

Purpose

Riboprobe generation

Depositor

GuangJun Zhang

Article

Park et al Dev Dyn. 2024 Jan 25. doi: 10.1002/dvdy.

Insert

kcnk2b (kcnk2b Zebrafish)

Use

Cloning

Promoter

T7

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pHWBF09exp

Plasmid

#130833

Purpose

Expression of plant LINC fluorescent fusion protein for cell biology

Depositor

Hank Bass

Article

Gumber et al J Cell Sci. 2019 Feb 11;132(3). pii: jcs

Insert

MLKP1

Tags

eGFP-FLAG-HA

Expression

Plant

Promoter

CaMV 35S

Available Since

Sept. 28, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
MLKP1delTM

Plasmid

#130841

Purpose

Expression of plant LINC fluorescent fusion protein for cell biology

Depositor

Hank Bass

Article

Gumber et al J Cell Sci. 2019 Feb 11;132(3). pii: jcs

Insert

MLKP1

Tags

eGFP-FLAG-HA

Expression

Plant

Mutation

Deletion of transmembrane domain

Promoter

CaMV 35S

Available Since

Sept. 28, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
MLKG1delKASH

Plasmid

#130842

Purpose

Expression of plant LINC fluorescent fusion protein for cell biology

Depositor

Hank Bass

Article

Gumber et al J Cell Sci. 2019 Feb 11;132(3). pii: jcs

Insert

MLKG1

Tags

eGFP-FLAG-HA

Expression

Plant

Mutation

Deletion of KASH domain

Promoter

CaMV 35S

Available Since

Sept. 28, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
MLKG1delKASHec

Plasmid

#130846

Purpose

Expression of plant LINC fluorescent fusion protein for cell biology

Depositor

Hank Bass

Article

Gumber et al J Cell Sci. 2019 Feb 11;132(3). pii: jcs

Insert

MLKG1

Use

Gateway entry vector

Tags

eGFP-FLAG-HA

Mutation

Deletion of KASH domain

Available Since

Sept. 28, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pHWBF07exp

Plasmid

#130832

Purpose

Expression of plant LINC fluorescent fusion protein for cell biology

Depositor

Hank Bass

Article

Gumber et al J Cell Sci. 2019 Feb 11;132(3). pii: jcs

Insert

MLKP2

Tags

eGFP-FLAG-HA

Expression

Plant

Promoter

CaMV 35S

Available Since

Sept. 28, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pKG-GW2-MaUPPS-B

Plasmid

#203175

Purpose

Yeast expression vector for accessory subunit of Methanosarcina acetivorans cis-prenyltransferase

Depositor

William Sessa

Article

Grabinska et al J Biol Chem. 2017 Oct 20;292(42):17351-1

Insert

MA_4402

Expression

Yeast

Promoter

TDH3

Available Since

Aug. 29, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pKG-GW1-MaUPPS-A

Plasmid

#203174

Purpose

Yeast expression vector for catalytic subunit of Methanosarcina acetivorans cis-prenyltransferase

Depositor

William Sessa

Article

Grabinska et al J Biol Chem. 2017 Oct 20;292(42):17351-1

Insert

uppS

Expression

Yeast

Promoter

TDH3

Available Since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti6.3/TO/V5-DEST-mTurquoise2

Plasmid

#191010

Purpose

Lentiviral expression vector for mTurquoise2

Depositor

Johanna Ivaska

Article

Conway et al Sci Adv. 2023 Jul 14;9(28):eadg1840. doi

Insert

mTurquoise2

Use

Lentiviral

Promoter

CMV

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLipoP1-NLS-3xmCitrine

Plasmid

#192522

Purpose

Fluorescent reporter for plant responses to bacterial infection

Depositor

Gitta Coaker

Article

Zhu et al Cell Rep. 2023 Jun 20;42(7):112676. doi:

Insert

Putative membrane lipoprotein (AT3G18250 Mustard Weed)

Tags

3xmCitrine and NLS

Expression

Plant

Promoter

LipoP1

Available Since

June 23, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pSLIK 3xFLAG-XRCC6 zeo

Plasmid

#192327

Purpose

Lentiviral expression vector for an inducible 3xFLAG-XRCC6

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

XRCC6 (XRCC6 Human)

Use

Lentiviral

Tags

3xFLAG

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pSLIK 3xFLAG-TTI1 zeo

Plasmid

#192328

Purpose

Lentiviral expression vector for an inducible 3xFLAG-TTI1

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

TTI1 (TTI1 Human)

Use

Lentiviral

Tags

3xFLAG

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pSLIK BirA* 3xFLAG-NUP37 zeo

Plasmid

#192333

Purpose

Lentiviral expression vector for an inducible 3xFLAG-BirA 3xFLAG-NUP37

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

BirA*-3xFLAG-NUP37 (NUP37 Human)

Use

Lentiviral

Tags

3xFLAG

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pSLIK mCh-V5-NES-EGFP-mCBP80 zeo

Plasmid

#192335

Purpose

Lentiviral expression vector for an inducible mCh-V5-NES-EGFP-mCBP80

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

mCh-V5-NES-EGFP-mCBP80

Use

Lentiviral

Tags

3xFLAG

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pSLIK mCh-V5-NES-EGFP-IBB zeo

Plasmid

#192336

Purpose

Lentiviral expression vector for an inducible mCh-V5-NES-EGFP-IBB

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

mCh-V5-NES-EGFP-IBB

Use

Lentiviral

Tags

3xFLAG

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pSLIK mCh-V5-NES-EGFP-SV40 NLS zeo

Plasmid

#192340

Purpose

Lentiviral expression vector for an inducible mCh-V5-NES-EGFP-SV40 NLS

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

mCh-V5-NES-EGFP-SV40 NLS

Use

Lentiviral

Tags

3xFLAG

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pSLIK 3xFLAG-NUP37 zeo

Plasmid

#192324

Purpose

Lentiviral expression vector for an inducible 3xFLAG-NUP37

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

NUP37 (NUP37 Human)

Use

Lentiviral

Tags

3xFLAG

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pSLIK 3xFLAG-PFN2_variant2 zeo

Plasmid

#192325

Purpose

Lentiviral expression vector for an inducible 3xFLAG-PFN2_variant2

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

PFN2_variant2 (PFN2 Human)

Use

Lentiviral

Tags

3xFLAG

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge

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