We narrowed to 7,031 results for: SIM
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Plasmid#240626PurposeExpresses a fusion construct containing a palmitoylation motif, mScarlet3 and 10 repeats of the SunTag (GCN4) peptide, to luminally tag extracellular vesicles for use in EV-FUSIM assay.DepositorInsertpalm-mScarlet3-10xSunTag
UseLentiviralExpressionMammalianPromoterEF1aAvailable SinceMarch 5, 2026AvailabilityAcademic Institutions and Nonprofits only -
pMDS1
Plasmid#239460PurposeFor simultaneous monitoring of both transcription and translation of genes in plant tissues: C-terminus fusionDepositorTypeEmpty backboneTagsVENUS:3xHA:2A:mTurqN7ExpressionBacterial and PlantAvailable SinceOct. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMDS2
Plasmid#239459PurposeSimultaneous monitoring of transcription and translation of genes in plant tissues: N- terminus fusionDepositorTypeEmpty backboneTags3xHA:VENUS:2A:mTurqN7ExpressionBacterial and PlantAvailable SinceAug. 29, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVM2-GoPGF-HD1
Plasmid#234909PurposeThis all-in-one vector is used to specifically silence the GoPGF and HD1 gene via virus-induced gene silencing (VIGS), simultaneously.DepositorInsertVIGS fragements of GoPGF and GhHD1
ExpressionPlantAvailable SinceJune 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRExT2A-NT-BmNG
Plasmid#232204PurposeEndogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for N-terminal tagging: BSD-3xTy-T2A-3xTy-mNeonGreenDepositorInsertmNeongreen
ExpressionBacterialAvailable SinceMarch 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRExT2A-NT-BmSc
Plasmid#232205PurposeEndogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for N-terminal tagging: BSD-3xTy-T2A-3xTy-mScarletDepositorInsertmScarlet
ExpressionBacterialAvailable SinceMarch 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRExT2A-CT-BmSc
Plasmid#232208PurposeEndogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for C-terminal tagging: mScarlet-3xTy-T2A-BSD-3xTyDepositorInsertmScarlet
ExpressionBacterialAvailable SinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRExT2A-NT-PmSc
Plasmid#232548PurposeEndogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for N-terminal tagging: PAC-3xTy-T2A-3xTy-mScarletDepositorInsertmScarlet
ExpressionBacterialMutationNoneAvailable SinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-hsAgo2
Plasmid#221718PurposeExpression of human Ago2 in zebrafish embryos.DepositorAvailable SinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV_Seq17_VS33_4.26_ChimeraIntron_3NLS_RFP_WPRE_polyA
Plasmid#220225PurposeAAV-STARR-seq validation vector containing VS33 enhancerDepositorInsertEnhancer (VS33)
UseAAVExpressionMammalianPromoterCMVAvailable SinceJuly 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xMmaPylT(UUA)_EF1_sfGFP150TAA
Plasmid#174897Purposeochre suppression reporter sfGFP150TAA expression, with MmaPylT(UUA) ochre suppressor tRNA cassetteDepositorInsertsf GFP
ExpressionMammalianMutation150TAA in sfGFPPromoterEF1Available SinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xMmaPylT(UCA)_EF1_sfGFP150TGA
Plasmid#174898Purposeopal suppression reporter sfGFP150TGA expression, with MmaPylT(UCA) opal suppressor tRNA cassetteDepositorInsertsfGFP
ExpressionMammalianMutation150TGA in sfGFPPromoterEF1Available SinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENTR4 Ta-SRO1 JN177
Plasmid#192546PurposepENTR plasmid Triticum aestivum SRO1, full CDS, cultivar JN177, no stop codonDepositorInsertSIMILAR TO RCD ONE 1 (SRO1), cultivar Jinan 177 (JN177)
UseGateway entry vectorAvailable SinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENTR4 Ta-sro1 SR3
Plasmid#192547PurposepENTR plasmid Triticum aestivum sro1, full CDS, cultivar SR3, no stop codonDepositorInsertSIMILAR TO RCD ONE 1 (SRO1), cultivar Shanrong No. 3 (SR3)
UseGateway entry vectorAvailable SinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
NK10 SFFV-mCherry-SG(s70587-3xMS2)SG-EGFP (FLP-IN)
Plasmid#191165PurposeExpression of a sensor for synthetic sequence 1 (EK0208) with three MS2 sequences in the coding sequence in mammalian cells. Similar to EK0438, but with MS2 sequences directly after the sensor sequence, rather than 3' UTRDepositorInsertmCherry:s70587-3xMS2:EGFP
ExpressionMammalianMutationWTPromoterSFFVAvailable SinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC19_4xT790_28bp
Plasmid#114720Purpose4 x 28 bp repeats of the EGFR WT allele used as SiMREPS analyte standardDepositorInsertEGFR (EGFR Human)
ExpressionBacterialAvailable SinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19_T790_28bp
Plasmid#114722Purpose28 bp fragment of the WT EGFR allele used as SiMREPS analyte standardDepositorInsertEGFR (EGFR Human)
ExpressionBacterialAvailable SinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(Q48T)
Plasmid#112725PurposeThis mutant of TBP6.7 binds to TAR very similarly to TBP6.7(WT), with only 1.4-fold reduced Kd.DepositorInsert6His-TEV-TBP6.7(Q48T)
Tags6His-TEVExpressionBacterialMutationQ48TPromoterT7Available SinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(P51A)
Plasmid#112728PurposeSimilar to P46, P51 was selected and is present in all evolved TBPs. Mutating this position into Ala has a modest effect on binding.DepositorInsert6His-TEV-TBP6.7(P51A)
Tags6His-TEVExpressionBacterialMutationP51APromoterT7Available SinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 mCherry Gamma 3
Plasmid#65234PurposeTo study the role of the differential rates of Gβγ translocation in modulating signaling dynamics in a cellDepositorAvailable SinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Alpha-0 Dronpa
Plasmid#44193DepositorInsertG protein alpha 0 (Gnao1 Rat)
TagsDronpaExpressionMammalianMutationIntroduced KpnI and SpeI sites between 92nd and 9…PromoterCMVAvailable SinceApril 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
VEE-mScarlet3-IRES-E3-NSs-L*-EGFP-IRES-srIκBα
Plasmid#242411PurposeSelf-amplifying RNA (saRNA) construct expresses dsRNA pathway inhibitors (E3, NSs, L*) and inflammatory signaling inhibitor, super repressor inhibitor of κBα (srIκBα).DepositorInsertsmScarlet3
IRES-E3-NSs-L*-EGFP
IRES-srIκBα
UseMouse Targeting; Self-amplifying rnaExpressionMammalianMutationserines 32 and 36 to alaninesAvailable SinceSept. 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xMx PylT_FLAG-Mx PylRS
Plasmid#140011PurposePlasmid with 4xMx PylT cassette and MX1201 PylRS for amber suppression; for transient or stable piggyBac-mediated integrationDepositorInsertMx1201 PylRS (MMALV_RS05520 Methanomethylophilus alvus Mx1201)
TagsFLAGExpressionMammalianPromoterEF1Available SinceMay 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xBstTyrT(CUA)_EcoTyrRS-FLAG
Plasmid#140018PurposePlasmid with 4xBstTyrT(CUA) cassette and E. coli TyrRS for amber suppression; for transient or stable piggyBac-mediated integrationDepositorInsertEcoTyrRS
TagsFLAGExpressionMammalianMutationY37L, D182S, F183M, L186APromoterEF1Available SinceMay 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide Puro-P2A-EGFP
Plasmid#137729PurposeFor simple determination of the multiplicity of infection (MOI) of a lentiviral CRISPR library by checking EGFP expression (still allowing for puro selection).DepositorTypeEmpty backboneUseCRISPR, Lentiviral, and Synthetic BiologyTagsEGFPExpressionBacterial and MammalianAvailable SinceFeb. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
[#GS15-LV] DsRed GFP TEVp Blast Switch - Recipient
Plasmid#233506PurposeLentiviral construct for the MitoTRACER genetic reporter to be expressed in the recipient cellsDepositorInsertDsRed loxp eGFP
UseLentiviral and Synthetic BiologyExpressionMammalianAvailable SinceJune 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-FAM20C:V5
Plasmid#63584PurposeFAM20C can phosphorylate secreted phosphoproteins, and characterization of its activity identifies FAM20C as a Golgi casein kinase.DepositorAvailable SinceNov. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
[#GS7-LV] DsRed GFP TEVp Zeo Switch - Recipient
Plasmid#233507PurposeLentiviral construct for the MitoTRACER genetic reporter to be expressed in the recipient cellsDepositorInsertDsRed loxP eGFP
UseLentiviral and Synthetic BiologyExpressionMammalianAvailable SinceJune 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pORTMAGE-2
Plasmid#72677PurposeExpresses Lambda Red recombinases and a dominant negative MutL allele all controlled by temperature sensitve cI857 repressor for high precision and efficiency MAGE experiments. Ap resistance marker.DepositorInsertmutL E32K
TagsnoneExpressionBacterialMutationE32K mutation conferring dominant mutator phenoty…PromoterpL promoterAvailable SinceFeb. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xEcoLeuT(CUA)_EF1_AnapRS
Plasmid#174894PurposeAnapRS expression for amber suppression in mammalian cells; transient or piggy bac mediated integrationDepositorInsertAnapRS
ExpressionMammalianMutationL38F, M40G, L41P, Y499V, Y500L, Y527A, H537E, L53…PromoterEF1Available SinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only