We narrowed to 22,191 results for: his

1. pYES2.1-cerMET17

   Plasmid

   #107443

   Purpose

   Overexpression of MET fusion protein in S. cerevisiae

   Depositor

   Clare O'Connor

   Article

   Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

   Insert

   MET17 (MET17 Budding Yeast)
   

   Use

   Tags

   V5-His

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available Since

   April 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. C-Ag15

   Plasmid

   #11868

   Depositor

   Martin Smith

   Article

   Hoover et al J Cell Biol. 2003 Jun 9. 161(5):923-32.

   Insert

   Agrn (Agrn Mouse)
   

   Use

   Tags

   His and myc

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 19, 2006

   Availability

   Academic Institutions and Nonprofits only

3. pFAST NPC1(NTD)-3Q

   Plasmid

   #25772

   Depositor

   Johann Deisenhofer

   Article

   Kwon et al Cell. 2009 Jun 26. 137(7):1213-24.

   Insert

   npc1 (NPC1 Human)
   

   Use

   Tags

   His

   Expression

   Insect

   Mutation

   aa23-252 only; N70Q N122Q N185Q

   Promoter

   Available Since

   Dec. 3, 2010

   Availability

   Academic Institutions and Nonprofits only

4. pCellFree_G03 S100A1

   Plasmid

   #67048

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   S100A1 (S100A1 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Expression

   Mutation

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pcDNA3-FRB-LplA(W37I)

   Plasmid

   #34840

   Depositor

   Alice Ting

   Article

   Slavoff et al J Am Chem Soc. 2011 Dec 14;133(49):19769

   Insert

   FRB-LplA(W37I)
   

   Use

   Tags

   FLAG (internal fusion), HA, and His Tag

   Expression

   Mammalian

   Mutation

   LplA has Trp37 to Val mutation

   Promoter

   CMV

   Available Since

   Feb. 22, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. C-Ag20(0)

   Plasmid

   #11866

   Depositor

   Martin Smith

   Article

   Hoover et al J Cell Biol. 2003 Jun 9. 161(5):923-32.

   Insert

   Agrn (Agrn Mouse)
   

   Use

   Tags

   His and myc

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 19, 2006

   Availability

   Academic Institutions and Nonprofits only

7. pET15b-pknG

   Plasmid

   #102814

   Purpose

   Expression of PknG in E.coli

   Depositor

   Jean Pieters

   Article

   Walburger et al Science. 2004 Jun 18;304(5678):1800-4. E

   Insert

   pknG
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Dec. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. f1H ∂HD (267)

   Plasmid

   #24426

   Depositor

   Steven Rosen

   Article

   Morimoto-Tomita et al Neoplasia. 2005 Nov . 7(11):1001-10.

   Insert

   SULF1 (SULF1 Human)
   

   Use

   Tags

   FLAG, His, and Myc

   Expression

   Mammalian

   Mutation

   AA 416-726 deleted, 22AA N-terminal truncation

   Promoter

   Available Since

   Feb. 10, 2012

   Availability

   Academic Institutions and Nonprofits only

9. pYES2.1-cerMET5

   Plasmid

   #107437

   Purpose

   Overexpression of MET fusion protein in S. cerevisiae

   Depositor

   Clare O'Connor

   Article

   Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

   Insert

   MET5 (MET5 Budding Yeast)
   

   Use

   Tags

   V5-His

   Expression

   Yeast

   Mutation

   V81I, K341E, P597L, Y605C, E741G, D932G, N1217S, …

   Promoter

   GAL1

   Available Since

   Aug. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCellFree_G03 YY1

    Plasmid

    #67082

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    YY1 (YY1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pCellFree_G03 PRKCA

    Plasmid

    #67115

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    PRKCA Protein kinase C alpha type (PRKCA Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

12. C-Ag20(8)

    Plasmid

    #11867

    Depositor

    Martin Smith

    Article

    Hoover et al J Cell Biol. 2003 Jun 9. 161(5):923-32.

    Insert

    Agrn (Agrn Mouse)
    

    Use

    Tags

    His and myc

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 19, 2006

    Availability

    Academic Institutions and Nonprofits only

13. pKM744

    Plasmid

    #134225

    Purpose

    Baculovirus expression of CENP-T and CENP-W

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

    Insert

    his-CENP T + CENP W
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    Feb. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pKM499

    Plasmid

    #134216

    Purpose

    Baculovirus expression of CENP-U CENP-Q CENP-OP

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

    Insert

    his-CENPU + CENPQ+ CENPOP
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJCSUP35(254-684)

    Plasmid

    #1092

    Depositor

    Susan Lindquist

    Article

    Glover et al Cell 1997 May 30;89(5):811-9.

    Insert

    SUP35 (SUP35 Budding Yeast)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    amino acids 254 - 684 of SUP35

    Promoter

    Available Since

    Jan. 21, 2005

    Availability

    Academic Institutions and Nonprofits only

16. pDEST51-LRRK2-R1441H

    Plasmid

    #25083

    Depositor

    Mark Cookson

    Article

    J Biol Chem. 2008 Jun 13;283(24):16906-1

    Insert

    LRRK2 (LRRK2 Human)
    

    Use

    Destination vector

    Tags

    6XHis and V5

    Expression

    Mammalian

    Mutation

    Arg 1441 mutated to His

    Promoter

    Available Since

    June 17, 2010

    Availability

    Academic Institutions and Nonprofits only

17. pET28b-AF0623

    Plasmid

    #30164

    Depositor

    SeWon Suh

    Article

    Kim do et al Acta Crystallogr Sect F Struct Biol Crys

    Insert

    AF0623 (lig Archaeoglobus fulgidus)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 19, 2012

    Availability

    Academic Institutions and Nonprofits only

18. pfast NPC1(NTD)

    Plasmid

    #25773

    Depositor

    Johann Deisenhofer

    Article

    Kwon et al Cell. 2009 Jun 26. 137(7):1213-24.

    Insert

    npc1 (NPC1 Human)
    

    Use

    Tags

    His

    Expression

    Insect

    Mutation

    aa 23-252 only

    Promoter

    Available Since

    Dec. 3, 2010

    Availability

    Academic Institutions and Nonprofits only

19. pCIneoFH human RASSF6

    Plasmid

    #40344

    Depositor

    Yutaka Hata

    Article

    Ikeda et al Sci Signal. 2009 Sep 29;2(90):ra59.

    Insert

    RASSF6 (RASSF6 Human)
    

    Use

    Tags

    FLAG and His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

20. pQStrep2-PIR

    Plasmid

    #31570

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    pirin (PIR Human)
    

    Use

    Tags

    His and StrepII-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pJK220

    Plasmid

    #71123

    Purpose

    Expression of E. coli pantotheinate kinase (H6PanK, R175H mutant) in E. coli

    Depositor

    T. J. Kappock

    Article

    Francois et al Biochemistry. 2006 Nov 14;45(45):13487-9

    Insert

    pantothenate kinase
    

    Use

    Tags

    H6

    Expression

    Bacterial

    Mutation

    Arg175 is mutated to a His

    Promoter

    T7

    Available Since

    Jan. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pClneoFH human RASSF3

    Plasmid

    #37019

    Depositor

    Yutaka Hata

    Article

    Ikeda et al Sci Signal. 2009 Sep 29;2(90):ra59.

    Insert

    RASSF3 (RASSF3 Human)
    

    Use

    Tags

    FLAG and His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 23, 2012

    Availability

    Academic Institutions and Nonprofits only

23. pCellFree_G03 PPARG

    Plasmid

    #67099

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    PPARG (PPARG Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

24. DPAGT1 (DPAGT1A-c005)

    Plasmid

    #98228

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    DPAGT1A (DPAGT1 Human)
    

    Use

    Baculovirus

    Tags

    His-6-TEV

    Expression

    Mutation

    pfam00953:Glycos_transf_4 98-275

    Promoter

    polyhedrin

    Available Since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCellFree_G03 PSMC1

    Plasmid

    #67087

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    PSMC1 (PSMC1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    E275G, I287V and R294G

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

26. pJCSUP35(123-254)

    Plasmid

    #1090

    Depositor

    Susan Lindquist

    Article

    Glover et al Cell 1997 May 30;89(5):811-9.

    Insert

    SUP35 (SUP35 Budding Yeast)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    amino acids 123 - 254

    Promoter

    Available Since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

27. pETDuet RagA-RagC(L91P)

    Plasmid

    #99647

    Purpose

    Bacterial expression of RagA-RagC(L91P)

    Depositor

    Kuang Shen, David Sabatini

    Article

    Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

    Inserts

    RagA (RRAGA Human)
    RagC (RRAGC Human)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    L91P

    Promoter

    Available Since

    Nov. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET28b-pchA WT

    Plasmid

    #87102

    Purpose

    expresses PchA WT, the isochorismate synthase from Pseudomonas aeruginosa

    Depositor

    Audrey Lamb

    Article

    Meneely et al Arch Biochem Biophys. 2013 Oct 1;538(1):

    Insert

    pchA WT
    

    Use

    Tags

    his-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pUCBB-lacZa

    Plasmid

    #32563

    Depositor

    Claudia Schmidt-dannert

    Article

    Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

    Insert

    LacZ-alpha
    

    Use

    Synthetic Biology

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Consitutive lac

    Available Since

    Nov. 8, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pfMDH_H303A

    Plasmid

    #67730

    Purpose

    Expresses H303A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli

    Depositor

    Justin Siegel

    Article

    Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:

    Insert

    Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
    

    Use

    Tags

    6x-His tag

    Expression

    Bacterial

    Mutation

    Changed histidine 303 to alanine

    Promoter

    T7

    Available Since

    Sept. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

31. pfMDH_D230A

    Plasmid

    #67725

    Purpose

    Expresses D230A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli

    Depositor

    Justin Siegel

    Article

    Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:

    Insert

    Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
    

    Use

    Tags

    6x-His tag

    Expression

    Bacterial

    Mutation

    Changed aspartate 230 to alanine

    Promoter

    T7

    Available Since

    Sept. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

32. pfMDH_N191A

    Plasmid

    #67724

    Purpose

    Expresses N191A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli

    Depositor

    Justin Siegel

    Article

    Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:

    Insert

    Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
    

    Use

    Tags

    6x-His tag

    Expression

    Bacterial

    Mutation

    Changed asparagine 191 to alanine

    Promoter

    T7

    Available Since

    Sept. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

33. pfMDH_K295A

    Plasmid

    #67726

    Purpose

    Expresses K295A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli

    Depositor

    Justin Siegel

    Article

    Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:

    Insert

    Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
    

    Use

    Tags

    6x-His tag

    Expression

    Bacterial

    Mutation

    Changed lysine 295 to alanine

    Promoter

    T7

    Available Since

    Sept. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

34. pFA3

    Plasmid

    #46790

    Purpose

    To express ATPase inactive Fun30 in budding yeast

    Depositor

    Patrick Varga-Weisz

    Article

    Neves-Costa et al PLoS One. 2009 Dec 1;4(12):e8111. doi: 1

    Insert

    FUN30 (FUN30 Budding Yeast)
    

    Use

    Tags

    V5

    Expression

    Yeast

    Mutation

    ATP-binding site mutated replaced lysin 603 by ar…

    Promoter

    PGAL1

    Available Since

    Aug. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

35. pNM3746

    Plasmid

    #154109

    Purpose

    loxP FLP FRT FRT3 landing site in a Golden Gate SapI GCG ACG assembly vector

    Depositor

    Michael Nonet

    Article

    Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

    Inserts

    LoxP-SEC-LoxP
    FRT::rpl-28p::GFP::His-58::FRT3
    mex-5p::FLP D5::sl2::mNG::glh2 3'
    

    Use

    Cre/Lox; Rmce

    Tags

    Expression

    Worm

    Mutation

    Promoter

    not applicable

    Available Since

    July 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCellFree_G03 GTF2H2D

    Plasmid

    #67081

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    GTF2H2D (GTF2H2C_2 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

37. pBbA5k-EPL3

    Plasmid

    #45401

    Depositor

    Aindrila Mukhopadhyay

    Article

    Dunlop et al Mol Syst Biol. 2011 May 10;7:487. doi: 1

    Insert

    mexF, Avin_33870 (mexF Synthetic)
    

    Use

    Synthetic Biology

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    LacUV5

    Available Since

    Dec. 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. L3MBTL3 (3UT1)

    Plasmid

    #36901

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    L3MBTL3 (L3MBTL3 Human)
    

    Use

    Tags

    His tag and Streptavidin-Binding Peptide (SBP)-Ta…

    Expression

    Bacterial

    Mutation

    contains amino acid residues 228-551 only

    Promoter

    Available Since

    June 21, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

39. pCellFree_G03 RAP2A

    Plasmid

    #67058

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    RAP2A (RAP2A Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

40. pET11a-TAX-L10-STEPtag

    Plasmid

    #159375

    Purpose

    Expresses STEPtag fused to the C-terminus of inactive T. aurantiacus xylanase 10A, as a mock protein of interest.

    Depositor

    Roberto Chica

    Article

    Eason et al ACS Synth Biol. 2020 Nov 20;9(11):2955-2

    Insert

    TAX-L10-STEPtag
    

    Use

    Tags

    6x His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Sept. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge