We narrowed to 40,634 results for: tro

1. pET-6xHis-GFP(+18)

   Plasmid

   #199162

   Purpose

   used to express a supercharged GFP variant in E. coli

   Depositor

   Allie Obermeyer

   Article

   Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

   Insert

   GFP(+18)
   

   Use

   Tags

   MGHHHHHHGGA

   Expression

   Mutation

   E16R, D29K, E42K, E44K, D86K, D112K, T115K, D127R…

   Promoter

   T7

   Available since

   May 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJBL7005

   Plasmid

   #136944

   Purpose

   Malachite green aptamer with stability hairpin, T7 promoter

   Depositor

   Julius Lucks

   Article

   Silverman et al ACS Synth Biol. 2019 Feb 15;8(2):403-414

   Insert

   Malachite green aptamer
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. +36GFP-LPETG

   Plasmid

   #71754

   Purpose

   +36GFP-LPETG for expression in E.Coli

   Depositor

   David Liu

   Article

   Li et al J Am Chem Soc. 2015 Nov 11;137(44):14084

   Insert

   (GGS)9_(pos36GFP)_6XHis_LPETG
   

   Use

   Tags

   6XHis and Sortase (LPETG)

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pQCXIP GFP-TREX1

   Plasmid

   #164245

   Purpose

   retroviral plasmid for GFP-TREX1

   Depositor

   John Maciejowski

   Article

   Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

   Insert

   TREX1 (TREX1 Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFS461

   Plasmid

   #89064

   Purpose

   leu1 integration plasmid with adh1pr:Z3EV

   Depositor

   Nick Rhind

   Article

   Ohira et al Yeast. 2017 Aug;34(8):323-334. doi: 10.1

   Insert

   Z3EV artificial transcription factor
   

   Use

   Leu1 integrating vector

   Tags

   Expression

   Mutation

   Promoter

   adh1

   Available since

   July 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pREDusk-AmpR-MCS

   Plasmid

   #188974

   Purpose

   For red light-repressed bacterial expression of gene of interest (AmpR)

   Depositor

   Heikki Takala

   Article

   Multamaki et al ACS Synth Biol. 2022 Aug 23. doi: 10.102

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET14b – lambda exonuclease

   Plasmid

   #104531

   Purpose

   bacterial expression of lambda exonuclease

   Depositor

   Charles Bell

   Article

   Zhang et al Proc Natl Acad Sci U S A. 2011 Jul 19;10

   Insert

   λ exonuclease
   

   Use

   Tags

   His6 followed by a thrombin site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. shNg pA_RC3J1_CAGW

   Plasmid

   #92155

   Purpose

   Bicistronic AAV construct encoding GFP control and shRNA targeting Ng

   Depositor

   Weifeng Xu

   Article

   Han et al Cell Rep. 2017 Jan 24;18(4):892-904. doi

   Insert

   shRNA targeting Ng (NRGN Human)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   H1

   Available since

   March 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDONR221-SLC25A22

   Plasmid

   #166210

   Purpose

   Gateway entry vector for N. furzeri SLC25A22

   Depositor

   Daniel Jarosz

   Article

   Chen et al Dev Cell. 2024 Jul 22;59(14):1892-1911.e

   Insert

   SLC25A22
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJM019

    Plasmid

    #134158

    Purpose

    Retroviral plasmid expressing GFP PSMA3

    Depositor

    Iain Cheeseman

    Article

    Monda et al Mol Biol Cell. 2018 Sep 15;29(19):2336-2

    Insert

    PSMA3 (PSMA3 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. mscv2.2-NAIP1

    Plasmid

    #60200

    Purpose

    Mouse NAIP1 expressed under the constitutive, moderate-level retroviral LTR promoter, expresses in mammalian cells; IRES-GFP reporter downstream of NAIP1

    Depositor

    Russell Vance

    Article

    Kofoed et al Nature. 2011 Aug 28;477(7366):592-5. doi

    Insert

    NAIP1
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    Feb. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLG014 (RADAR)

    Plasmid

    #157892

    Purpose

    Expresses the RADAR defense system

    Depositor

    Feng Zhang

    Article

    Gao et al Science. 2020 Aug 28;369(6507):1077-1084

    Insert

    RADAR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET51b(+)_ALFA-tag-SNAP

    Plasmid

    #136628

    Purpose

    Plasmid encoding the bacterial expression of ALFA-tag asa SNAP-tag fusion

    Depositor

    Kai Johnsson

    Article

    Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

    Insert

    EGFP-NbALFA
    

    Use

    Tags

    Hisx10 and Strep

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAV-CAG-LSL-mCherry-shPtbp1

    Plasmid

    #178591

    Purpose

    Cre-dependent expression of mCherry and a shRNA against Ptbp1 under the constitutive promoter

    Depositor

    Chun-Li Zhang

    Article

    Wang et al Cell. 2021 Oct 14;184(21):5465-5481.e16.

    Insert

    miRE-Ptbp1
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTlpA-Wasabi

    Plasmid

    #85455

    Purpose

    mWasabi reporter gene gated by wild type TlpA

    Depositor

    Mikhail Shapiro

    Article

    Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

    Inserts

    TlpA
    mWasabi
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    D341A

    Promoter

    TlpA and TlpA, LacI

    Available since

    Feb. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTlpA-Wasabi-NF

    Plasmid

    #86100

    Purpose

    Nonfluorescent variant of mWasabi reporter gene gated by wild type TlpA

    Depositor

    Mikhail Shapiro

    Article

    Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

    Inserts

    TlpA
    mWasabi
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    D341A and S71T, G73A

    Promoter

    TlpA and TlpA, LacI

    Available since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. mscv2.2-NAIP2

    Plasmid

    #60201

    Purpose

    Mouse NAIP2 expressed under the constitutive, moderate-level retroviral LTR promoter, expresses in mammalian cells; IRES-GFP reporter downstream of NAIP2

    Depositor

    Russell Vance

    Article

    Kofoed et al Nature. 2011 Aug 28;477(7366):592-5. doi

    Insert

    NAIP2 (Naip2 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBA615

    Plasmid

    #186239

    Purpose

    eLbuCas13a Negative Control (RFP targeting)

    Depositor

    Jennifer Doudna

    Article

    Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

    Insert

    pTet-LbuCas13a + crRNA negative control
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFlpStop-HDR-UAS-2.1-tdTom

    Plasmid

    #89148

    Purpose

    FlpStop plasmid for CRISPR/HDR. Has MCS for homology arms

    Depositor

    Tom Clandinin

    Article

    Fisher et al Elife. 2017 Feb 17;6. pii: e22279. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    5XUAS, 3XP3-dsRed

    Available since

    April 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti-CaMKIIa-ChETA-EYFP

    Plasmid

    #26967

    Purpose

    3rd gen lentiviral expression of humanized ChR2 with E123T/H134R mutations fused to EYFP driven by CaMKIIa promoter for optogenetic activation

    Depositor

    Karl Deisseroth

    Article

    Gunaydin et al Nat Neurosci. 2010 Mar;13(3):387-92. doi

    Insert

    hChR2(E123T/H134R)-EYFP
    

    Use

    Lentiviral

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    E123T

    Promoter

    CaMKIIa

    Available since

    Jan. 11, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pJKR-H-cdaR

    Plasmid

    #62557

    Purpose

    Glucarate biosensor. High copy plasmid that expresses GFP proportional to glucarate concentration.

    Depositor

    George Church

    Article

    Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

    Inserts

    beta lactamase
    sfGFP
    CdaR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pRAF-MutL

    Plasmid

    #86607

    Purpose

    E. coli MutL expression plasmid

    Depositor

    Richard Fishel

    Article

    Liu et al Nature. 2016 Nov 16;539(7630):583-587. d

    Insert

    E. coli MutL
    

    Use

    Tags

    his-tag and ald-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pENTR hH1-TERRA

    Plasmid

    #111381

    Purpose

    TERRA with approximately 130 telomere hexanucleotide repeats of TTAGGG and TTGGGG variant sequences under control of hH1 promoter and terminator

    Depositor

    Klaus Holzmann

    Article

    Kreilmeier et al Genes (Basel). 2016 Aug 16;7(8). pii: ge

    Insert

    hH1-TERRA
    

    Use

    Gateway entry cloning

    Tags

    Expression

    Mutation

    Promoter

    hH1

    Available since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pNR64

    Plasmid

    #79540

    Purpose

    Input plasmid: expresses two recombinases downstream of two inducible promoters. Same as Dual-Recombinase-Controller (plasmid #44456) from Endy Lab except with KanR instead of CamR on the backbone.

    Depositor

    Timothy Lu

    Article

    Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

    Inserts

    TP901
    BxbI
    

    Use

    Tags

    His tag and LAA degradation tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAG1244 p65-mCherry-FIREtag

    Plasmid

    #205175

    Purpose

    Expresses p65-mCherry-FIREtag in mammalian cells

    Depositor

    Arnaud Gautier

    Article

    Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

    Insert

    p65-mCherry-FIREtag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. hMSH2 Clone E3 (full-length)

    Plasmid

    #16453

    Depositor

    Bert Vogelstein

    Article

    Leach et al Cell. 1993 Dec 17. 75(6):1215-25.

    Insert

    MSH2 (MSH2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pQCXIP-GFP-RPA70

    Plasmid

    #164231

    Purpose

    retroviral plasmid for GFP-RPA70

    Depositor

    John Maciejowski

    Article

    Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

    Insert

    RPA70 (RPA1 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAG1238 PINK1-mCherry-FIREtag

    Plasmid

    #205174

    Purpose

    Expresses PINK1-mCherry-FIREtag in mammalian cells

    Depositor

    Arnaud Gautier

    Article

    Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

    Insert

    PINK1-mCherry-FIREtag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. FCK-QuasAr2-Citrine

    Plasmid

    #59172

    Purpose

    Fluorescent membrane voltage sensor

    Depositor

    Adam Cohen

    Article

    Zou et al Nat Commun. 2014 Aug 13;5:4625. doi: 10.

    Insert

    QuasAr2-Citrine eFRET
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCAGGS-N2c(G)

    Plasmid

    #73481

    Purpose

    N2c Glycoprotein expression vector

    Depositor

    Thomas Jessell

    Article

    Reardon et al Neuron. 2016 Jan 19. pii: S0896-6273(16)

    Insert

    N2c(G)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 21, 2016

    Availability

    Academic Institutions and Nonprofits only

31. pRAF-MutH

    Plasmid

    #86609

    Purpose

    E. coli MutH expression plasmid

    Depositor

    Richard Fishel

    Article

    Liu et al Nature. 2016 Nov 16;539(7630):583-587. d

    Insert

    E. coli MutH
    

    Use

    Tags

    his-tag and ald-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pRAF-MutS

    Plasmid

    #86605

    Purpose

    E. coli MutS expression plasmid

    Depositor

    Richard Fishel

    Article

    Liu et al Nature. 2016 Nov 16;539(7630):583-587. d

    Insert

    E. coli MutS (D835R,R840E)
    

    Use

    Tags

    his-tag and ald-tag

    Expression

    Bacterial

    Mutation

    D835R, R840E

    Promoter

    T7

    Available since

    Feb. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCAG-NL1AB

    Plasmid

    #15262

    Depositor

    Peter Scheiffele

    Article

    Chih et al Neuron. 2006 Jul 20. 51(2):171-8.

    Insert

    Neuroligin 1 with inserts A and B (Nlgn1 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. Trip-GFP-hOCLN

    Plasmid

    #32789

    Depositor

    Tianyi Wang

    Article

    Liu et al Virology. 2010 Nov 10;407(1):160-70. Epu

    Insert

    OCLN (OCLN Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 1, 2011

    Availability

    Academic Institutions and Nonprofits only

35. pcDNA3.1(-) FLIPQTV3.0 2m

    Plasmid

    #63731

    Purpose

    FLIPQTV3.0 glutamine sensor with R75MW220A mutations

    Depositor

    Sakiko Okumoto

    Article

    Gruenwald et al PLoS One. 2012;7(6):e38591. doi: 10.1371

    Insert

    FLIPQTV3.0 sensor, 2mM affinity
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pMK392 (mAID-mClover-BSD)

    Plasmid

    #121193

    Purpose

    C-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

    Insert

    mAID-mClover-BS
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. hSDH5

    Plasmid

    #113046

    Purpose

    Expression of WT hSDH5

    Depositor

    Edward A. Berry

    Article

    Zafreen et al Arch Biochem Biophys. 2016 Aug 15;604:47

    Insert

    hSDH5 (SDHAF2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Insert begins at amino acid 39

    Promoter

    Available since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. HA-BICD2(1-500)-FRB

    Plasmid

    #174648

    Purpose

    Rapalog-inducible coupling to dynein adaptor BICD2

    Depositor

    Lukas Kapitein

    Article

    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    HA-BICD2(1-500)-FRB
    

    Use

    Tags

    FRB and HA

    Expression

    Mammalian

    Mutation

    mmBICD2(aa1-500): Lys213Glu

    Promoter

    Chicken beta-actin

    Available since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pTJV1ScKm-rpoB

    Plasmid

    #166980

    Purpose

    Generates ssDNA in vivo targeting E. coli rpoB for recombineering w/ Beta recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; npt2 for kanamycin resistance

    Depositor

    Christopher Reisch

    Article

    Ellington et al Synth Biol (Oxf). 2022 May 3;7(1):ysac00

    Insert

    NeoR/KanR
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pTcI-Wasabi

    Plasmid

    #86101

    Purpose

    mWasabi reporter gene gated by temperature-sensitive Lambda repressor TcI

    Depositor

    Mikhail Shapiro

    Article

    Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

    Inserts

    TcI
    mWasabi
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    A67T

    Promoter

    pR/pL and pR/pL, LacI

    Available since

    Feb. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge