We narrowed to 7,670 results for: 11
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Plasmid#134362PurposeNanoluc complementation assay. Expression of Gαs protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 113 and 114 of Gαs. Addition of the HA epitope at N terminus of Gαs.DepositorAvailable SinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only
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pEGFPN1-gamma-tubulin
Plasmid#87956PurposeExpresses human gamma-tubulin with a GFP-tag on the C-terminal of the proteinDepositorInsertgamma-tubulin 1 (TUBG1 Human)
TagsGFPExpressionMammalianMutationThe protein is a sh-gamma-tubulin resistant gene …Available SinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1.Gαi3-LgB91
Plasmid#134359PurposeNanoluc complementation assay. Expression of Gαi3 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 91 and 92 of Gαi3. Addition of the HA epitope at N terminus of Gαi3.DepositorAvailable SinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
STARR-seq luciferase INF enhancer vector_ORI_OAS3
Plasmid#99324PurposeLuciferase validation vector with OAS3 enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activityDepositorInsertenhancer; chr12: 94575894 -94578286
UseLuciferase; Starr-seq luciferase validation vectorExpressionMammalianAvailable SinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-SMS2-TEV-Twin-Strep
Plasmid#202529PurposeExpress C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged human Sphingomyelin synthase 2 (SMS2) in mammalian cellDepositorInsertSGMS2 (SGMS2 Human)
TagsTEV cleavable site and Twin-Strep-tagExpressionMammalianMutationdeleted stop codon (TGA)PromoterCAG and chicken β-actin promoterAvailable SinceJuly 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
STARR-seq luciferase validation vector_ORI_IGF1R
Plasmid#99306PurposeLuciferase validation vector with IGF1R enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activityDepositorInsertenhancer; chr15: 99439352 -99440791
UseLuciferase; Starr-seq luciferase validation vectorExpressionMammalianAvailable SinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-EphB1
Plasmid#200981PurposeMammalian expression plasmid for myc-tagged EphB1DepositorInsertEphB1 (EPHB1 Human)
TagsExtracellular c-myc epitope tag and Signal peptid…ExpressionMammalianPromoterCMVAvailable SinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1.Gαi2-LgB91
Plasmid#134358PurposeNanoluc complementation assay. Expression of Gαi2 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 91 and 92 of Gαi2. Addition of the HA epitope at N terminus of Gαi2.DepositorAvailable SinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pWPI_SPbFGF
Plasmid#25812DepositorInsertpWPI_SPbFGF (FGF2 Human)
UseLentiviralMutationIg signal peptide in N-terminal of human FGF2Available SinceJan. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCMV-dCas9-mD3A
Plasmid#78257PurposeExpresses dead Cas9 (dCas9) fused to inactive DNMT3A catalytic domain (CD) under the control of the CMV promoterDepositorInsertDNMT3A CD aa 598-912 (DNMT3A Human)
UseCRISPRTagsFlag epitope tagExpressionMammalianMutationE756APromoterpCMVAvailable SinceJune 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNCST-AausGFP
Plasmid#129503PurposeExpresses AausGFP constitutively in E. coli (most strains)DepositorInsertAausGFP
ExpressionBacterialPromotersynthetic constitutive (stationary phase) promote…Available SinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
OTR untagged
Plasmid#208913Purposeencodes the human oxytocin receptor, without any tagsDepositorAvailable SinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-dCas9-D3A
Plasmid#78256PurposeExpresses dead Cas9 (dCas9) fused to DNMT3A catalytic domain (CD) under the control of the CMV promoterDepositorInsertDNMT3A CD aa 598-912 (DNMT3A Human)
UseCRISPRTagsFlag epitope tagExpressionMammalianPromoterpCMVAvailable SinceJune 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB_eCBmut
Plasmid#164605PurposeExpress the endocannabinoid non-responsive sensor GRAB_eCBmut in neuronsDepositorInsertEndocannabinoid non-responsive sensor GRAB_eCBmut
UseAAVMutationS383T, F177APromoterhSynAvailable SinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMX-SHP1C/S-ires-mCD8
Plasmid#183719PurposeExpresses the myc-tagged SHP-1 C453S mutant (SHP-1C/S) (substrate-trapping mutant) and mouse CD8.DepositorInsertSHP-1
UseRetroviralTagsmycMutationC453SAvailable SinceJune 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-SMS1-TEV-HAT
Plasmid#202557PurposeExpress C-terminal Tobacco Etch Virus (TEV) protease cleavable HAT-tagged human Sphingomyelin synthase 1 (SMS1) in mammalian cellDepositorInsertSGMS1 (SGMS1 Human)
TagsHistidine-Affinity-tag (HAT) and TEV cleavable si…ExpressionMammalianMutationdeleted stop codon (TAA)PromoterCAG and chicken β-actin promoterAvailable SinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
FH1-MGT#1-mVenus
Plasmid#164094PurposeFluorescent reporter for genetic tracing of mesenchymal Glioblastoma cell-state.DepositorInsertMGT#1-mVenus
UseLentiviral and Synthetic BiologyAvailable SinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-MCHpr-GCaMP6f
Plasmid#221039PurposeExpresses GCaMP6f under the rat pro-melanin-concentrating hormone promoterDepositorInsertGCaMP6f
UseAAV; Rat targetingExpressionMammalianMutationA317EPromoterRat MCHAvailable SinceAug. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET41b+_Npl4
Plasmid#117108PurposeProduction of recombinant Npl4 in E.coliDepositorInsertNpl4 (NPLOC4 Human)
ExpressionBacterialAvailable SinceOct. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
MT-AvrXa10-FT
Plasmid#234373PurposeT-DNA vector for expression of the two protein components of the MoonTag system (dCas9-24XGP41 and NbGP41P-GFP-AvrXa10-GB1) with optimized activation domain (AvrXa10), targeting Arabidopsis FT locusDepositorInsertsNbGP41-GFP-AvrXa10-GB1
dCas9-24XGP41
gRNAs targeting FT locus
RFP
UseSynthetic BiologyTagsGB1, GFP, and GP41ExpressionPlantPromoterArabidopsis U6, Arabidopsis Ubi10, and Arabidopsi…Available SinceApril 17, 2025AvailabilityAcademic Institutions and Nonprofits only