We narrowed to 33,378 results for: IND

1. pHR-PD-1 (BTLA ITIM)-mGFP

   Plasmid

   #180817

   Purpose

   Expression of human PD-1 with immunoreceptor tyrosine-based inhibitory motif (ITIM) from BTLA, fused to mEGFP

   Depositor

   Enfu Hui

   Article

   Xu et al Elife. 2021 Nov 4;10. pii: 74276. doi: 1

   Insert

   PD-1 (BTLA ITIM) (PDCD1 Human)
   

   Use

   Lentiviral

   Tags

   mEGFP

   Mutation

   replaced PD-1-ITIM (VDYGEL) with BTLA-ITIM (IVYAS…

   Promoter

   SFFV

   Available Since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV.U6gT28.4.hSyn.flex.H2B.RFP

   Plasmid

   #170386

   Purpose

   Plasmid used for Crispr/cas9 based disruption of mouse Trim28, guide targeting exon 4. Cre-inducible expression of nuclear RFP.

   Depositor

   Johan Jakobsson

   Article

   Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

   Insert

   Cre inducible H2B RFP
   

   Use

   AAV

   Promoter

   human Synapsin

   Available Since

   Jan. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV.U6gT28.3.hSyn.flex.H2B.RFP

   Plasmid

   #170374

   Purpose

   Plasmid used for Crispr/cas9 based disruption of mouse Trim28, guide targeting exon 3. Cre-inducible expression of nuclear RFP.

   Depositor

   Johan Jakobsson

   Article

   Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

   Insert

   Cre inducible H2B RFP
   

   Use

   AAV

   Promoter

   human Synapsin

   Available Since

   Jan. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV.U6gT28.13.hSyn.flex.H2B.RFP

   Plasmid

   #170376

   Purpose

   Plasmid used for Crispr/cas9 based disruption of mouse Trim28, guide targeting exon 13. Cre-inducible expression of nuclear RFP.

   Depositor

   Johan Jakobsson

   Article

   Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

   Insert

   Cre inducible H2B RFP
   

   Use

   AAV

   Promoter

   human Synapsin

   Available Since

   Jan. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. NmMetQ C20A

   Plasmid

   #172112

   Purpose

   Expression of Methionine Binding Protein (MetQ) with C20A mutation in signal sequence

   Depositor

   Douglas Rees

   Article

   Sharaf et al Elife. 2021 Aug 19;10. pii: 69742. doi:

   Insert

   Methionine Binding Protein
   

   Tags

   10x Histidine Tag and N.meningitidis signal seque…

   Expression

   Bacterial

   Mutation

   Changed Proline 22 to Alanine on MetQ and Cystein…

   Promoter

   T7 Promoter

   Available Since

   Oct. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCu-His6-Atg13[571–700]-Ss(424)

   Plasmid

   #166852

   Purpose

   Expresses 6xHis tagged ATG13 [571–700] fragment with 14 scramble amino acid sequence DIKLIDTVDLESCN under a Cu promoter in yeast.

   Depositor

   Daniel Klionsky

   Article

   Gatica et al Autophagy. 2020 Jun;16(6):1007-1020. doi

   Insert

   Atg13[571–700] (ATG13 Budding Yeast)
   

   Tags

   6xHis

   Expression

   Yeast

   Promoter

   pCu

   Available Since

   April 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRS405 SEC66-VAC8-GFP

   Plasmid

   #166840

   Purpose

   Expresses ER localized Vac8-GFP in yeasts. Made replacing the first 21 base pairs of the VAC8 ORF with the first 162 base pairs from the SEC66 ORF, which encode the Sec66 transmembrane domain.

   Depositor

   Daniel Klionsky

   Article

   Gatica et al Autophagy. 2020 Jun 8. doi: 10.1080/1554

   Insert

   VAC8 (VAC8, SEC66 Budding Yeast)
   

   Tags

   GFP and SEC66 transmembrane domain

   Expression

   Yeast

   Mutation

   SEC66 transmembrane domain

   Promoter

   VAC8 promoter

   Available Since

   April 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. CMV-10xMS2 no wild type

   Plasmid

   #158204

   Purpose

   Cassette of 10 different binding sites with high affinity to MS2 phage coat protein, as extracted form the oligo pool experiment. It does not contain the MS2-wt sequence, therefore QCP cannot bind it.

   Depositor

   Roee Amit

   Article

   Katz et al Nat Commun. 2021 Mar 11;12(1):1576. doi:

   Insert

   10 binding sites of MS2 without the WT sequence from oligo library experiment
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. CMV-10xQb no wild type

   Plasmid

   #158205

   Purpose

   Cassette of 10 different binding sites with high affinity to Qb phage coat protein, as extracted form the oligo pool experiment. It does not contain the Qb-wt sequence, therefore MCP cannot bind it.

   Depositor

   Roee Amit

   Article

   Katz et al Nat Commun. 2021 Mar 11;12(1):1576. doi:

   Insert

   10 binding sites of QB coat protein without the WT sequence based on the oligo library experiment
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-BRCA1-RNF169UBD

    Plasmid

    #119009

    Purpose

    Mammalian expression of a fusion protein of human BRCA1 with the human RNF169 Ubiquitin binding domain and BFP expression

    Depositor

    Ralf Kuehn

    Article

    Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

    Insert

    BRCA1 (BRCA1 Human)
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-BRCA1-Rad18UBD

    Plasmid

    #119008

    Purpose

    Mammalian expression of a fusion protein of human BRCA1 with human Rad18 Ubiquitin binding domain and BFP expression

    Depositor

    Ralf Kuehn

    Article

    Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

    Insert

    BRCA1 (BRCA1 Human)
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pQLink-GST-2xCTD

    Plasmid

    #138470

    Purpose

    Expresses GST-tagged 2xCTD heptapeptide repeat in bacteria

    Depositor

    Britt Glaunsinger

    Article

    Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

    Insert

    Rpb1 (POLR2A Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    2x CTD repeat

    Promoter

    T5

    Available Since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLB(N)CX-FLAG-CH3 Del33

    Plasmid

    #133724

    Purpose

    Retroviral vector with CMV promoter driving mutant form of CH3 domain fragment of human p300 (EP300), with N-terminal FLAG tag.

    Depositor

    James A. DeCaprio

    Article

    Borger et al J Virol. 2006 May . 80(9):4292-303.

    Insert

    EP300 (EP300 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Mutation

    partial (not complete) p300 fragment, AT and CH3 …

    Promoter

    CMV

    Available Since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. EF1a-3NLS-Sox2TALE-GCN4

    Plasmid

    #120548

    Purpose

    Express 3xNLS-Sox2 TALE-GCN4 engineered to bind a site in the human SOX2 gene

    Depositor

    Yu Wang

    Article

    Zhao et al ACS Chem Biol. 2018 Mar 16;13(3):609-617

    Insert

    NLS, Sox2 TALE, GCN4 (SOX2 Synthetic)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1α

    Available Since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. EF1a-3NLS-NGN2TALE-GCN4

    Plasmid

    #120547

    Purpose

    Express 3xNLS-NGN2 TALE-GCN4 engineered to bind a site in the human NGN2 ene

    Depositor

    Yu Wang

    Article

    Zhao et al ACS Chem Biol. 2018 Mar 16;13(3):609-617

    Insert

    NLS, NGN2 TALE, GCN4 (NEUROG2 Synthetic)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1α

    Available Since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pVL43 - pNST3>>

    Plasmid

    #116009

    Purpose

    destination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expression

    Depositor

    Thomas Greb

    Article

    Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

    Insert

    pNST3:GR-LhG4:tNST3:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
    

    Tags

    HDEL and SP(ER)

    Expression

    Plant

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pVL42 - pLTP1>>

    Plasmid

    #116008

    Purpose

    destination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expression

    Depositor

    Thomas Greb

    Article

    Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

    Insert

    pLTP1:GR-LhG4:tLT1:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
    

    Tags

    HDEL and SP(ER)

    Expression

    Plant

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA5-puro-eGFP-HEC1-M4

    Plasmid

    #114036

    Purpose

    Expression of HEC1 M4 mutant tagged with GFP

    Depositor

    Geert Kops

    Article

    Hiruma et al Science. 2015 Jun 12;348(6240):1264-7. d

    Insert

    GFP tagged - HEC1 -M4 (NDC80 Human)
    

    Expression

    Mammalian

    Mutation

    M4 (K146E, R153E, K156E)

    Promoter

    CMV TetO

    Available Since

    Sept. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA5-puro-eGFP-HEC1-M2

    Plasmid

    #114035

    Purpose

    Expression of HEC1 M2 mutant tagged with GFP

    Depositor

    Geert Kops

    Article

    Hiruma et al Science. 2015 Jun 12;348(6240):1264-7. d

    Insert

    GFP tagged - HEC1 -M2 (NDC80 Human)
    

    Expression

    Mammalian

    Mutation

    M2 (K81E, N87E, K89E)

    Promoter

    CMV TetO

    Available Since

    Sept. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET28a_6HT-TBP6.7(R47A)

    Plasmid

    #112722

    Purpose

    Arg47 within TBP6.7 is the most critical residue in terms of forming interactions with WT TAR. Mutating this amino acid to Ala results in ~600-fold reduction in Kd.

    Depositor

    Joseph Wedekind

    Article

    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

    Insert

    6His-TEV-TBP6.7(R47A)
    

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    R47A

    Promoter

    T7

    Available Since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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