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pHCMV_GP64

Plasmid

#207276

Purpose

Mammalian expression of baculovirus GP64 protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

GP64

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_HaloTag9_LamB1

Plasmid

#175538

Purpose

Expression of HaloTag9 at the nuclear lamina of the mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

HaloTag9-LamB1

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_b4g-HaloTag9

Plasmid

#175546

Purpose

Expression of HaloTag9 at the Golgi apparatus of mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

Beta4Gal-HaloTag9

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_NES_HaloTag9

Plasmid

#175537

Purpose

Strict cytosolic expression of HaloTag9 in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

NES-HaloTag9

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pMC-9-35S-11

Plasmid

#197759

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

35S terminator

Expression

Plant

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pMC-1-35S_Long-6

Plasmid

#197698

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

35S promoter (1.3kb)

Expression

Plant

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1_3XFlag

Plasmid

#182494

Purpose

Empty plasmid containing Flag tag for C-terminal tagging

Depositor

Dogus Altintas, Paolo Comoglio

Article

Cerqua et al Life Sci Alliance. 2022 May 30;5(10). pi

Insert

3xFlag with 5 Glycolic spacer

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pCDH-TMEM106b-mApple

Plasmid

#179385

Purpose

Lentiviral vector for expression of TMEM106b fused mApple

Depositor

Angus Johnston

Article

Rennick et al Nat Commun. 2022 Oct 12;13(1):6023. doi:

Insert

TMEM106b - mApple

Use

Lentiviral

Tags

mApple

Expression

Mammalian

Promoter

EF1

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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L4440_BioBrick-sgRNA

Plasmid

#177783

Purpose

Overexpression of sgRNAs in E. coli HT115, sgRNA overexpression scaffold; for ingestion by C. elegans

Depositor

Michael Ristow

Article

Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

Type

Empty backbone

Expression

Bacterial

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-GFP1 POD-nAb-WPRE

Plasmid

#244973

Purpose

Expresses a fusion protein of a nanobody against GFP and peroxidase (POD) in mammalian cells

Depositor

Hiroyuki Hioki

Article

Yamauchi et al Commun Biol. 2025 Jun 18;8(1):903. doi:

Insert

GFP1 POD-nAb

Available Since

Oct. 20, 2025

Availability

Academic Institutions and Nonprofits only

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RpHLuorin2

Plasmid

#180227

Purpose

for bacterial expression of recombinant ratiometric pHluorin2

Depositor

Geert van den Bogaart

Article

Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

Insert

Ratiometric pHLuorin2

Tags

His-tag followed by thrombin cleavage site

Expression

Bacterial

Promoter

T7

Available Since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

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pSFFV_mNG3A(1-10)

Plasmid

#157992

Purpose

Lentiviral vector of constitutive expression of mNeonGreen3A(1-10)

Depositor

Bo Huang

Article

Zhou et al PLoS One. 2020 Nov 23;15(11):e0242592. d

Insert

pSFFV_mNG3K(1-10)

Use

Lentiviral

Expression

Mammalian

Mutation

W148L, M150V, K153M, N194K, F204L

Promoter

SFFV

Available Since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_CalR_HaloTag7_KDEL

Plasmid

#175530

Purpose

Expression of HaloTag7 in the endoplasmic reticulum of mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

CalR-HaloTag7-KDEL

Expression

Mammalian

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_IgKchL_HA_HaloTag7_myc_PDGFRtmb

Plasmid

#175534

Purpose

Expression of HaloTag7 at the outer leaflet of the plasma membrane of mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

IgKchL_HA-HaloTag7-myc_PDGFRtmb

Expression

Mammalian

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_[Cox8a]x2_HaloTag7

Plasmid

#175529

Purpose

Expression of HaloTag7 at the inner membrane of mitochondria in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

Cox8a-HaloTag7

Expression

Mammalian

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-) Cystector (Cysteine biosensor)

Plasmid

#250653

Purpose

Cytosolic expression plasmid for Cystector (Cysteine biosensor) in mammalian cells.

Depositor

Peter Dedecker

Article

Pedre et al bioRxiv 2026.01.27.698081

Insert

Cystector (Cysteine biosensor)

Expression

Mammalian

Promoter

CMV

Available Since

March 24, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_Lyn11-HaloTag7

Plasmid

#175533

Purpose

Expression of HaloTag7 at the inner leaflet of the plasma membrane of mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

Lyn11-HaloTag7

Expression

Mammalian

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_Beta4Gal-HaloTag7

Plasmid

#175536

Purpose

Expression of HaloTag7 at the at the Golgi apparatus of mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

Beta4Gal-HaloTag7

Expression

Mammalian

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pRpHLuorin2-N1

Plasmid

#171716

Purpose

Ratiometric pHLuorin2 backbone (C terminal fusion)

Depositor

Geert van den Bogaart

Article

Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

Type

Empty backbone

Expression

Mammalian

Available Since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

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pET246-pUC57 12xMS2V6

Plasmid

#104390

Purpose

MS2V6 cloning

Depositor

Robert Singer, Evelina Tutucci

Article

Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

Insert

12xMS2V6

Expression

Bacterial

Mutation

All Loops are U-variants, interspaced by 50 nucle…

Available Since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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