We narrowed to 4,011 results for: plasmid lenti crispr

1. pLenti_PEmax-2A-MLH1dn-2A-BSD

   Plasmid

   #234072

   Purpose

   For lentiviral expression of PEmax with MLH1dn and blasticidin resistance

   Depositor

   Greg Findlay

   Article

   Herger et al Cell Genom. 2025 Mar 18:100814. doi: 10.

   Insert

   PEmax-2A-MLH1dn
   

   Use

   CRISPR and Lentiviral

   Tags

   SV40 bpNLS and c-Myc NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1α core promoter

   Available since

   March 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. LentiAsCpf1-3xMYC-blast

   Plasmid

   #127750

   Purpose

   Lentiviral plasmid for expressing human codon-optimized AsCpf1 containing 3x N-terminal MYC-NLS signal

   Depositor

   Giulio Draetta

   Article

   Liu et al Nat Commun. 2019 Jul 17;10(1):3144. doi:

   Insert

   AsCpf1
   

   Use

   CRISPR, Lentiviral, and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-hU6-cs1-PuroR-T2A-BFP

   Plasmid

   #230934

   Purpose

   gRNA cloning vector with direct capture sequence 1 in stem loop 2

   Depositor

   Charles Gersbach

   Article

   Liu et al Cell Genom. 2025 Feb 4:100766. doi: 10.1

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   TagBFP

   Expression

   Mutation

   Promoter

   Available since

   Feb. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. lenti SYN-SVI-DIO-dCas9-KRAB-MeCP2

   Plasmid

   #170378

   Purpose

   Expresses a SYN-driven, intron-containing dCas9-KRAB-MeCP2 fusion in which the part of the dCas9 cassette is double floxed and in inverted orientation (DIO). Requires Cre-dependent recombination.

   Depositor

   Jeremy Day

   Article

   Carullo et al eNeuro. 2021 Jul 26. pii: ENEURO.0188-21

   Insert

   A DIO FLAG-dCas9-KRAB-MeCP2 cassette containing an SV40 intron
   

   Use

   CRISPR, Cre/Lox, and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   human Synapsin promoter

   Available since

   May 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. Direct-seq_lentiGuide-Puro-tRNA-Tetra Loop-8A8G

   Plasmid

   #157985

   Purpose

   Expresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.

   Depositor

   Lijia Ma

   Article

   Song et al Genome Biol. 2020 Jun 8;21(1):136. doi:

   Insert

   S. pyogenes sgRNA cassette (tRNA-Tetra-8A8G)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   May 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti-hU6-enhanced_gRNA-BFP-P2A-PuroR

   Plasmid

   #230935

   Purpose

   gRNA cloning vector encoding BFP and puromycin resistance

   Depositor

   Charles Gersbach

   Article

   Liu et al Cell Genom. 2025 Feb 4:100766. doi: 10.1

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   TagBFP

   Expression

   Mutation

   Promoter

   hU6

   Available since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-PE2max-BSD

   Plasmid

   #191102

   Purpose

   Lentiviral expression plasmid of PEmax prime editor with P2A-BSD marker

   Depositor

   Hyongbum Kim

   Article

   Yu et al Cell. 2023 Apr 21:S0092-8674(23)00331-8.

   Insert

   PEmax-P2A-BSD
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Changed R221K, N394K, H840A from SpCas9

   Promoter

   EF-1a

   Available since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti-NRCH-PE2-BSD

   Plasmid

   #191101

   Purpose

   Lentiviral expression plasmid of NRCH-PE prime editor with P2A-BSD marker

   Depositor

   Hyongbum Kim

   Article

   Yu et al Cell. 2023 Apr 21:S0092-8674(23)00331-8.

   Insert

   NRCH-PE2-BSD
   

   Use

   CRISPR and Lentiviral

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. plenti-Lb-Cas12a-2xNLS

   Plasmid

   #155046

   Purpose

   Lentiviral vector expressing human codon-optimized _C-terminal Myc-tagged Lachnospiraceae bacterium (Lb)-Cas12a nuclease with N- and C-terminal NLS and Neomycin/G418/Geneticin resistance from EF-1alpha promoter

   Depositor

   Benjamin Blencowe, Jason Moffat

   Article

   Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

   Insert

   LbCas12a
   

   Use

   CRISPR and Lentiviral

   Tags

   Nucleoplasmin NLS, Myc-tag and SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   Aug. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. plenti-As-Cas12a-2xNLS

    Plasmid

    #155047

    Purpose

    Lentiviral vector expressing human codon-optimized _C-terminal Myc-tagged _Acidaminococcus sp. BV3L6 (As)-Cas12a nuclease with N- and C-terminal NLS and Neomycin/G418/Geneticin resistance from EF-1alpha promoter

    Depositor

    Benjamin Blencowe, Jason Moffat

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    AsCas12a
    

    Use

    CRISPR and Lentiviral

    Tags

    Nucleoplasmin NLS, Myc-tag and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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